2021
DOI: 10.1016/j.mimet.2021.106277
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Detection of hydrocarbon-degrading bacteria on deepwater corals of the northeast Atlantic using CARD-FISH

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Cited by 4 publications
(1 citation statement)
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“…To date, HCR-FISH has been used to overcome high autofluorescence to visualize bacteria in tissue sections in one coral study (Wada et al 2022 ), but it has also been applied to anemones (Goffredi et al 2021 ) and the bobtail squid (Nikolakakis et al 2015 , Moriano-Gutierrez et al 2019 ). CARD-FISH has been used on histology sections to better resolve bacteria within autofluorescent shallow (Chiu et al 2012 , Neave et al 2016 ) and deep-water (Thompson and Gutierrez 2021 ) coral tissues, but this approach requires reagents that damage DNA (Keller and Pollard 1977 ). CARD-FISH would therefore not be suitable for post-labelling metagenomic applications.…”
Section: Resultsmentioning
confidence: 99%
“…To date, HCR-FISH has been used to overcome high autofluorescence to visualize bacteria in tissue sections in one coral study (Wada et al 2022 ), but it has also been applied to anemones (Goffredi et al 2021 ) and the bobtail squid (Nikolakakis et al 2015 , Moriano-Gutierrez et al 2019 ). CARD-FISH has been used on histology sections to better resolve bacteria within autofluorescent shallow (Chiu et al 2012 , Neave et al 2016 ) and deep-water (Thompson and Gutierrez 2021 ) coral tissues, but this approach requires reagents that damage DNA (Keller and Pollard 1977 ). CARD-FISH would therefore not be suitable for post-labelling metagenomic applications.…”
Section: Resultsmentioning
confidence: 99%