Detection of hypoxia and inflammatory processes in tissues by fluorescence spectroscopy in vivo

Petritskaya, E. N.; Abaeva, L. F.; Lapitan, Denis G.; Kulikov, Dmitriy; Smirnova, O. D.; Guseva, I. A.; Рогаткин, Д. А.

doi:10.1109/lo.2014.6886461

Cited by 1 publication

(1 citation statement)

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“…Recently, some indirect data confirming the hypothesis of enhanced accumulation of exogenous fluorophores of an aluminum phthalocyanine series in inflammed tissues were published (Petritskaya et al, 2014). Enhanced fluorescence was observed at local inflammation due to invasion of external substances into tissues.…”

Section: Introductionmentioning

confidence: 92%

In Vivo Experimental Detection of Inflammatory Process in Tissues by Fluorescence Spectroscopy

Guseva

Рогаткин

Глазкова

et al. 2016

Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies

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Laser fluorescence spectroscopy (LFS) is widely used in medicine. Today, oncology and oncosurgery are considered as the most promising fields of its application. It is known that cancerous tissues are able to accumulate different porphyrins, both endogenous and exogenous, in enhanced amount due to increased metabolism in cancerous cells. So, LFS can be used in vivo for detection of malignant tumours as well as for real-time intraoperative imaging or diagnostics at a photodynamic therapy. One of the reason of the enhanced accumulation of porphyrins in tissues is a chronic hypoxia. Therefore, it was hypothesized, that LFS could also be used for diagnosis of local inflammation in tissues. Recently, some indirect data confirming the hypothesis was obtained when observed inflammation due to invasion of external substances into tissues. This study proves the hypothesis in a direct experiment with animals and laboratory tests. Enhanced fluorescence intensity of the exogenous photosensitizer was found in inflamed tissues. The direct association between intensity of the fluorescence, histological characteristics and blood test results was shown. It was found that the registered fluorescence signal correlates with neutrophil counts in blood of tested animals. It proves that LFS could be an effective tool for registration of local inflammation.

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Section: Introductionmentioning

confidence: 92%