Detection of <i>Corynebacterium salmoninus</i> by Direct Fluorescent Antibody Test

Bullock, G. L.; Griffin, Billy R.; Stuckey, H. M.

doi:10.1139/f80-092

Cited by 86 publications

(46 citation statements)

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“…Several methods have been compared for the detection of the causative agent in carrier broodstocks , Elliott et al 1989, Sakai et al 1989). Due to the ease of application, the most common method has been examination of kidney smears by the fluorescent antibody test (FAT) which replaced the less sensitive Gram stain (Bullock & Stuckey 1975, Bullock et al 1980). More recently, the enzyme-linked immunosorbent assay (ELISA) has been shown to be the most sensitive, accurate, quantitative, and easily automated test for large-scale detection of Rs soluble antigens in salmonids , Turaga et al 1987, Sakai et al 1989, Hsu et al 1991, Pascho et al 1991.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for measuring the prevalences and levels of Renibacterium salmoninarum in wild and hatchery stocks of salmonid fishes in Alaska. USA

Meyers¹,

Short²,

Farrington³

et al. 1993

Dis. Aquat. Org.

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ABSTRACT. The enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) were compared for their sensitivity in detection of Renibacterjum salmoninarum (Rs) in kidney tissues of Alaskan salmonids. The ELISA appeared to be more sensitive in detecting Rs infections. The FAT did not detect Rs in 80 % of the ELISA-positive samples but was positive for Rs in 28 'K, of the samples that were ELISA-negative. This contradiction may have been due to low-level washover of Rs cells from smears containing large numbers of Rs cells when slldes containing nlultiple samples were rinsed in d common vessel during the FAT procedures. The FAT routinely did not detect infections in Rs-positive fish the tissues of which produced a mean ELISA optical density value 50.173, and inconsistently detected infections in fish with ELISA values > 0.173 but < 0.978. The 0.978 optical density was the mean ELISA value at which the FAT routinely detected Rs-positive fish. Based on the ELlSA results, Rs occurred in only 9 % of the Alaskan Pacific salmon tested in both wild (85 "/U) and hatchery (81 %) stocks. The very high stock prevalences and levels of Rs antigen detected in wild trout Oncorhynch~~s spp., char Salvelinus spp., and grayling Thymallus arcticus having no clinical signs of bacterial kidney disease suggest these species may be somewhat resistant hosts and important freshwater reservoirs of Rs.

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Section: Introductionmentioning

confidence: 99%

Comparison of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for measuring the prevalences and levels of Renibacterium salmoninarum in wild and hatchery stocks of salmonid fishes in Alaska. USA

Meyers¹,

Short²,

Farrington³

et al. 1993

Dis. Aquat. Org.

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“…FRANTSI et al (1977) using adult return data and minimal disease diagnosis, reported on the possible negative effects of BKD on salmon returns. STUCKEY, 1975, CHEN et al, 1974;KIMURA et al, 1978;KIMURA, 1978;BULLOCK et al, 1980 BULLOCK and STUCKEY, 1975;KIMURA , 1978;LAIDLER, 1980;BULLOCK et al 1980). ( EARP et al 1953;WOOD and YASUTAKE, 1956;KAWAMURA et al, 1978).…”

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confidence: 99%

Studies on Bacterial Kidney Disease in Atlantic Salmon (Salmon salar) in Canada

Paterson¹,

Lall²,

Desautels³

1981

Fish Pathol.

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“…Bacterial cultures have been made more effective with the identification of the proper incubation time 4 as well as the development of KDM-2 medial 3,18,35 and has been used routinely for confirmation of this infection. Fluorescent antibody testing has been well established 2,8,9,11,19 and is used commonly as a screening tool to determine if fish are infected with BKD. Polyclona1 23,25,33 immunoenzyme assays, 26,28,37 and western blot analyses.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Diagnostic Tests for Bacterial Kidney Disease in Juvenile Steelhead Trout (Oncorhynchus Mykiss)

White

Albregts

1995

J VET Diagn Invest

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In order to accurately diagnose bacterial kidney disease caused by Renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (ELISA) test kit, fluorescent antibody (FA) testing, bacteriologic culture, and histopathology. Seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline ( n = 20), 1 × 1010 organisms/ml ( n = 18), 1 × 108 organisms/ml ( n = 18), or 1 × 106 organisms/ml ( n = 19) of R. salmoninarum. ELISA, FA, and bacteriologic culture were positive for R. salmoninarum from the kidney tissue of the 2 groups infected with the highest doses. Although the ELISA and FA tests were accurate when compared to the bacteriologic culture from the 2 groups infected with the higher doses of the organism, they were less sensitive at the lowest level of inoculum. Histopathology was not specific for this disease; however, all infected fish had a marked proliferative histiocytic interstitial nephritis, characterized by marked expansion of the renal hematopoietic tissue by histiocytes without tissue necrosis. Other microscopic findings included splenitis and myositis (at the injection site) of some fish.

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Detection of Corynebacterium salmoninus by Direct Fluorescent Antibody Test

Cited by 86 publications

References 0 publications

Comparison of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for measuring the prevalences and levels of Renibacterium salmoninarum in wild and hatchery stocks of salmonid fishes in Alaska. USA

Comparison of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for measuring the prevalences and levels of Renibacterium salmoninarum in wild and hatchery stocks of salmonid fishes in Alaska. USA

Studies on Bacterial Kidney Disease in Atlantic Salmon (Salmon salar) in Canada

Comparison of Diagnostic Tests for Bacterial Kidney Disease in Juvenile Steelhead Trout (Oncorhynchus Mykiss)

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