Detection of<i>Coxiella burnetii</i>DNA in Peridomestic and Wild Animals and Ticks in an Endemic Region (Canary Islands, Spain)

Bolaños-Rivero, Margarita; Carranza-Rodríguez, Cristina; Rodríguez, N.F.; Gutiérrez, Carlos; Pérez-Arellano, José-Luis

doi:10.1089/vbz.2017.2120

Cited by 36 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the results of the current study revealed that 10 (5.3%) adult ticks of H. dromedarii were infected with C. burnetii using PCR. This low Q fever infection agrees with that recorded before in ticks collected from animals in Spain [ 70 , 71 ]. The band size of IS30A spacer of C. burnetii was 200 bp in the positive samples of camel blood and tick tissues.…”

Section: Discussionsupporting

confidence: 92%

“…The infection rate of Q fever detected in camels by PCR was 15.85 % and 10.76% in Saudi Arabia and Iran, respectively [ 64 , 69 ]. In Spain, C. burnetii DNA was detected in 3.4 and 6.1% of ticks collected from animals at two different locations [ 70 , 71 ]. In Egypt, the present study is considered the first for molecular characterization of Q fever on either camels or ticks by PCR targeting the IS30A spacer.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Abdullah¹,

Shanawany²,

Abdel-Shafy³

et al. 2018

Vet World

View full text Add to dashboard Cite

Background and Aim:Q fever Coxiella burnetii is a worldwide zoonotic disease, and C. burnetii was detected in mammals and ticks. Ticks play an important role in the spread of C. burnetii in the environment. Therefore, the aims of this study were to detect Q fever C. burnetii in camels and ixodid ticks by molecular tools and identification of Hyalomma dromedarii and Hyalomma excavatum using molecular and immunological assays.Materials and Methods:A total of 113 blood samples from camels and 190 adult ticks were investigated for the infection with C. burnetii by polymerase chain reaction (PCR) and sequencing the targeting IS30A spacer. The two tick species H. dromedarii and H. excavatum were characterized molecularly by PCR and sequencing of 16S ribosomal RNA (16S rRNA) and cytochrome oxidase subunit-1 (CO1) genes and immunologically by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.Results:A total of 52 camels (46%) were positive for Q fever infection. Only 10 adult ticks of H. dromedarii were infected with C. burnetii. The IS30A sequence was around 200 bp in length for C. burnetii in H. dromedarii ticks with a similarity of 99% when compared with reference data in GenBank records. The length of 16S rDNA and CO1 was 440 and 850 bp, respectively, for both H. dromedarii and H. excavatum. The phylogenetic status of H. dromedarii was distant from that of H. excavatum. SDS-PAGE revealed seven different bands in the adult antigens of either H. dromedarii or H. excavatum with molecular weights ranged from 132.9 to 17.7 KDa. In western blot analyses, the sera obtained from either infested camel by H. dromedarii or infested cattle by H. excavatum recognized four immunogenic bands (100.7, 49.7, 43.9, and 39.6 kDa) in H. dromedarii antigen. However, the infested camel sera identified two immunogenic bands (117 and 61.4 kDa) in H. excavatum antigen. Furthermore, the sera collected from cattle infested by H. excavatum recognized three immunogenic bands (61.4, 47.3, and 35 kDa) in H. excavatum antigen.Conclusion:Molecular analyses indicated that both camels and ticks could be sources for infection of animals and humans with Q fever. Furthermore, the molecular analyses are more accurate tools for discriminating H. dromedarii and H. excavatum than immunological tools.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Abdullah¹,

Shanawany²,

Abdel-Shafy³

et al. 2018

Vet World

View full text Add to dashboard Cite

show abstract

“…It is remarkable that the incidence in Spain remains stable compared to another French study where the incidence shows a continuous increase (Frankel et al, 2011). This phenomenon might have several explanations: i) use of new techniques that increase the diagnostic possibilities (Bolaños-Rivero et al, 2017a), ii) a better knowledge of the disease and, therefore, of its suspicion, iii) a more adequate record of the number of cases, iv) different patterns of disease transmission (Bolaños-Rivero et al, 2017b), and v) the presence of outbreaks that increase the incidence of the disease. This French study shows a maximum incidence between March and August, which is identical to our data (Frankel et al, 2011).…”

Section: Incidence Ratesmentioning

confidence: 91%

Epidemiological scenario of Q fever hospitalized patients in the Spanish Health System: What’s new

Rodríguez‐Alonso

Almeida²,

Alonso‐Sardón

et al. 2020

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

A B S T R A C TObjectives: The objective of this study was to assess the epidemiology and burden of Q fever (QF) in Spain. Methods: We designed a retrospective descriptive study using the minimum basic data set in patients admitted to hospitals of the National Health System between 1998 and 2015 with a diagnosis of Q fever (ICD-9: 083.0.). Results: We found 4214 hospitalized patients with a mean age (AESD) of 50.9 AE 19.3 years. The male/ female ratio was 3:1. The incidence rate was between 0.41 and 0.65 cases per 100,000 person-years over the 18-year period. The highest incidence of cases was from March to August (p = 0.024). 21.1% patients had pneumonia, 17.5% had liver disease, and only 3.2% had endocarditis. The average hospital stay was 13.8 days (AE12.8). A total of 117 (2.8%) patients died. The total mean cost of QF is approximately s154,232,779 (s36,600 AE 139,422 per patient). Conclusions: QF is an important zoonosis in Spain with a stable incidence rate and high cost for hospitalization. Older patients have a more severe clinical picture and higher mortality, which can be decreased with early clinical suspicion.

show abstract

“…The brown dog tick was the most common species of tick found on domestic animals, and one positive sample originated from a domestic dog. The authors suggest that in this area C. burnetii circulate in the cycle which involves domestic animals, small rodents, lagomorphs, and ticks [31]. Whereas in central Spain the presence of C. burnetii was detected in both questing ticks (7.7%) and in ticks collected from domestic and wild animals (3.4%).…”

Section: Discussionmentioning

confidence: 89%

Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)

et al. 2018

View full text Add to dashboard Cite

Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecifi c transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confi rmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identifi ed: Rhipicephalus s anguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228) . The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.

show abstract

Detection ofCoxiella burnetiiDNA in Peridomestic and Wild Animals and Ticks in an Endemic Region (Canary Islands, Spain)

Cited by 36 publications

References 21 publications

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Epidemiological scenario of Q fever hospitalized patients in the Spanish Health System: What’s new

Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)

Contact Info

Product

Resources

About