Detection ofFusariumsp. contamination in corn by enzyme-linked immunosorbent assay

Abstract

Fusarium verticillioides is a primary corn pathogen and one of the main producers of fumonisins, a group of mycotoxins that cause several diseases in animals and probably also affect humans. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on polyclonal antibodies was developed to detect the exoantigen of this fungus in corn and its correlation with traditional methods for mould detection was evaluated. Forty freshly harvested corn samples were analysed for F. verticillioides exoantigens, as well as for total mould count, ergosterol and fumonisin levels in order to evaluate the relationship between these parameters. In addition, F. verticillioides was grown in brain heart infusion (BHI) broth in order to evaluate the correlation between biomass and exoantigen concentration. There was no significant correlation between exoantigen concentration and total mould count, Fusarium sp. count and fumonisin levels. The correlation coefficient between exoantigens and ergosterol content was 0.52 and between biomass and F. verticillioides exoantigens in BHI broth was 0.84. These results suggest that this ic-ELISA has potential for Fusarium sp. detection in corn samples.

Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

“…in food [32,33,34], few reports on ELISA for Fusarium sp. [20,22,35,36] were found to discuss the current data. Biazon et al [35] also produced polyclonal antibodies against F. verticillioides exoantigen, which showed cross reactivity with other Fusarium species, e.g., F. graminearum (51%), F. sporotrichioides (66%), and F. subglutinans (76%).…”

“…Despite many efforts, the ELISAs developed to date to detect Fusarium species in food are genus-specific [20,21,22].…”

Métodos imunológicos para a detecção de fungos toxigênicos e micotoxinas em grãos, alimentos e rações

“…O ELISA é o principal método imunológico desenvolvido para detectar espécies de Fusarium e é utilizado no monitoramento da eficiência de fungicidas, na determinação da resistência de linhagens de plantas e no controle de qualidade de grãos [52][53][54][55][56][57][58] .…”

“…Meirelles et al 54 desenvolveram um ic-ELISA utilizando anticorpos de coelho contra exoantígenos de F. verticillioides com o objetivo de quantificar exoantígenos de F. verticillioides em amostras de milho recém-colhido. O limite de detecção desse ensaio foi de 1,6 µg/g, e as concentrações de exoantígenos determinadas por esse método variaram de 8,9 µg/g a 965,0 µg/g, com valor médio de 217,3 µg/g.…”

Use of peanut waste for oyster mushroom substrate supplementation—oyster mushroom and peanut waste

“…Peanut (Arachis hypogaea, Leguminosae) is the fourth most planted and consumed oilseed worldwide. It also provides a good option for crop rotation [3,4]. Currently, several sugar cane reform areas are cultivated with peanut with the aim of improving the physical, chemical, and biological conditions of the soil.…”