Detection of<i>Naegleria fowleri</i>cysts in environmental samples by using a DNA probe

Sparagano, Olivier

doi:10.1111/j.1574-6968.1993.tb06474.x

Cited by 12 publications

(1 citation statement)

References 9 publications

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“…Conversely, RFLP (restriction fragment length polymorphism) is a diagnostic technique specific to species within the Naegleria genus based on distinct restriction patterns 35 – 37 . A DNA probe-based detection method, introduced in the mid-1990s, provides an alternative to these traditional approaches 38 . Nevertheless, these methods possess limitations, including time-intensive culturing, expensive RFLP, delayed diagnosis through ELISA, or insufficient outcomes from the FT.…”

Section: Genomic Profiling Techniques For Early Detectionmentioning

confidence: 99%

Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization of Naegleria fowleri infections leading to primary amebic meningoencephalitis (PAM)

Shaukat,

Khaliq,

Riaz

et al. 2024

Annals of Medicine &Amp; Surgery

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This review delves into the strategies for early detection and characterization of Naegleria fowleri infections leading to primary amoebic meningoencephalitis (PAM). The study provides an in-depth analysis of current diagnostic approaches, including cerebrospinal fluid analysis, brain tissue examination, immunostaining techniques, and culture methods, elucidating their strengths and limitations. It explores the geographical distribution of N. fowleri, with a focus on regions near the equator, and environmental factors contributing to its prevalence. The review emphasizes the crucial role of early detection in PAM management, discussing the benefits of timely identification in treatment, personalized care, and prevention strategies. Genomic profiling techniques, such as conventional PCR, nested PCR, multiplex PCR, and real-time PCR, are thoroughly examined as essential tools for accurate and prompt diagnosis. Additionally, the study explores advanced microscopic imaging techniques to characterize N. fowleri’s morphology and behavior at different infection stages, enhancing our understanding of its life cycle and pathogenic mechanisms. In conclusion, this review underscores the potential of these strategies to improve our ability to detect, understand, and combat N. fowleri infections, ultimately leading to better patient outcomes and enhanced public health protection.

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Section: Genomic Profiling Techniques For Early Detectionmentioning

confidence: 99%

Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization of Naegleria fowleri infections leading to primary amebic meningoencephalitis (PAM)

Shaukat,

Khaliq,

Riaz

et al. 2024

Annals of Medicine &Amp; Surgery

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Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri

Réveiller

Cabanes

Marciano‐Cabral

2002

Parasitology Research

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Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a fatal disease of the central nervous system that is acquired while swimming or diving in freshwater. A cDNA clone designated Mp2C15 obtained from N. fowleri was used as a probe to distinguish N. fowleri from other free-living amoebae. The Mp2C15 probe hybridized to genomic DNA from pathogenic N. fowleri and antigenically related non-pathogenic N. lovaniensis. Mp2C15 was digested with the restriction enzyme XbaI, resulting in two fragments, Mp2C15.G and Mp2C15.P. Four species of Naegleria and four species of Acanthamoeba were examined for reactivity with Mp2C15.P. Mp2C15.P was specific for N. fowleri and was used in the development of a nested PCR assay which is capable of detecting as little as 5 pg of N. fowleri DNA or five intact N. fowleri amoebae. In summary, a rapid, sensitive, and specific assay for the detection of N. fowleri was developed.

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The identification of Naegleria fowleri from water and soil samples by nested PCR

et al. 2004

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Naegleria fowleri is the causative agent of primary amebic meningoencephalitis (PAM), a disease of the central nervous system. Healthy humans sporadically become infected with N. fowleri and develop fatal PAM after recreational or work exposure to freshwater; accordingly, there is a need for monitoring the presence of pathogenic ameboflagellates in public freshwater. The present study was conducted to determine whether a nested PCR assay could be used for the detection of N. fowleri in freshwater habitats. Water samples were collected in Virginia, since Naegleria has been isolated previously in this state. Additionally, the occurrence of N. fowleri in samples from Connecticut was investigated since neither N. fowleri nor PAM has been reported from this region. PCR analysis demonstrated that two of four samples from Virginia were positive for N. fowleri without intervening culture while 15 of 86 samples from Connecticut were positive that had been enriched by culture. This constitutes the first report of N. fowleri from Connecticut waters. These results indicate that the PCR assay can be utilized to detect N. fowleri in water and soil collected from the environment.

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Detection ofNaegleria fowlericysts in environmental samples by using a DNA probe

Cited by 12 publications

References 9 publications

Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization of Naegleria fowleri infections leading to primary amebic meningoencephalitis (PAM)

Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization of Naegleria fowleri infections leading to primary amebic meningoencephalitis (PAM)

Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri

The identification of Naegleria fowleri from water and soil samples by nested PCR

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