Detection of <i>Renibacterium salmoninarum</i> and <i>Yersinia ruckeri</i> by the peroxidase‐antiperoxidase immunohistochemical technique in melanin‐containing cells of fish tissues

Jansson, Eva; Hongslo, T.; Lindberg, R.; Ljungberg, Otto; Svensson, B.-M.

doi:10.1111/j.1365-2761.1991.tb00628.x

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…The primary antibodies (diluted 1:200) used were rabbit anti‐ Y. ruckeri serotype O1 (Jansson et al . ); rabbit anti‐cod g‐lysozyme (Inami et al . ) and the previously described antibodies prepared at our laboratory (Magnadottir, Gudmundsdottir & Groman ); rabbit anti‐cod C3; mouse anti‐IgM; mouse anti‐CRP‐PI, mouse anti‐CRP‐PII and mouse anti‐ApoLP A1.…”

Section: Methodsmentioning

confidence: 99%

“…The primary antibodies (diluted 1:200) used were rabbit anti-Y. ruckeri serotype O1 (Jansson et al 1991); rabbit anti-cod g-lysozyme (Inami et al 2010) and the previously described antibodies prepared at our laboratory (Magnadottir, Gudmundsdottir & Groman 2013); rabbit anti-cod C3; mouse anti-IgM; mouse anti-CRP-PI, mouse anti-CRP-PII and mouse anti-ApoLP A1. A preimmune rabbit serum, normal mouse ascites and the conjugated antibody served as the relevant controls and also cod that were free of infection.…”

Section: Pathological Examinationmentioning

confidence: 99%

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Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions

Guðmundsdóttir

Magnadóttir

2013

Journal of Fish Diseases

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A disease outbreak in farmed Atlantic cod caused by Yersinia ruckeri is reported. Mortality started following vaccination of cod reared in two tanks (A and B). The accumulated mortality reached 1.9% in A and 4.8% in B in the following 30 days when treatment with oxytetracycline was applied. Biochemical and molecular analysis of Y. ruckeri isolates from the cod and other fish species from fresh and marine waters in Iceland revealed a high salinity-tolerant subgroup of Y. ruckeri serotype O1. Infected fish showed clinical signs comparable with those of Y. ruckeri -infected salmonids, with the exception of granuloma formations in infected cod tissues, which is a known response of cod to bacterial infections. Immunohistological examination showed Y. ruckeri antigens in the core of granulomas and the involvement of immune parameters that indicates a strong association between complement and lysozyme killing of bacteria. Experimental infection of cod with a cod isolate induced disease, and the calculated LD50 was 1.7 × 10(4) CFU per fish. The results suggest that yersiniosis can be spread between populations of freshwater and marine fish. Treatment of infected cod with antibiotic did not eliminate the infection, which can be explained by the immune response of cod producing prolonged granulomatous infection.

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Section: Methodsmentioning

confidence: 99%

Section: Pathological Examinationmentioning

confidence: 99%

Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions

Guðmundsdóttir

Magnadóttir

2013

Journal of Fish Diseases

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“…Com o vírus, a questão é mais interessante, pois já se podem realizar diagnósticos sobre tecidos fixados sem necessariamente realizar cultivos celulares. Todavia, a possibilidade de se contar com o tecido fixado e processado permite realizar estudos retrospectivos, por exemplo, sobre novas enfermidades de cuja existência anterior não se sabia por não serem descritas ou por não contarem com o anticorpo apropriado (Jansson, 1991;Hammer, 2007;Singh, 2008).…”

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Yersiniose em Trachinotus marginatus (pampo): diagnóstico histopatológico e imuno-histoquímico

Romano

Tesser

Sampaio

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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Descreveu-se um surto de yersiniose em uma criação de pampo Trachinotus marginatus estudado no Laboratório de Piscicultura Estuarina e Marinha da Universidade Federal do Rio Grande. A enfermidade manifestou-se por transtornos natatórios, exoftalmia com panoftalmite e pela clássica boca vermelha, nome pelo qual se denomina "enfermidade da boca vermelha" (EBV). Na necropsia, foram observados focos de hemorragia peritoneal, esplenomegalia e hepatomegalia. Microscopicamente, foi observada panoftalmite com infiltrados inflamatórios densos que afetavam quase todas as estruturas oculares. Esses infiltrados estavam constituídos por granulócitos, linfócitos, macrófagos e células granulares eosinofílicas. No exsudado, observaram-se estruturas pequenas, pouco coradas, de aspecto bacteriano Gram negativo. O estudo imuno-histoquímico, que se utilizou de um anticorpo monoclonal anti-Yersinia ruckeri, resultou positivo. Este é o primeiro surto conhecido de yersiniose em Trachinotus marginatus no Brasil.

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“…Albumin was not used for adherence of the specimen to the cucumber, as has been reported. 5 Utilization of the end paper during processing ensured adhesion of the specimen to the cucumber and serves as a small field to work in while embedding. This procedure can be employed for virtually any tissue specimen for which proper orientation or loss of a specimen during processing is a concern.…”

mentioning

confidence: 99%

“…Direct immunohistochemical procedures were performed as previously described, 3,5,10 Paraffin-embedded tissue sections were deparaffinized in an oven followed by 3 changes of toluene for 5 minutes each, 2 changes of 100% ethanol for 3 minutes each, and 2 changes of 95% ethanol for 3 minutes each. Slides were then rinsed in running water and incubated overnight in 5% hydrogen peroxide to remove melanin pigments.…”

mentioning

confidence: 99%

The Use of Kidney Biopsy of Broodstock Steelhead Trout (Oncorhyncus Mykiss) to Determine the Status of Bacterial Kidney Disease Infection

White

Albregts

et al. 1996

J VET Diagn Invest

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Detection of Renibacterium salmoninarum and Yersinia ruckeri by the peroxidase‐antiperoxidase immunohistochemical technique in melanin‐containing cells of fish tissues

Cited by 13 publications

References 6 publications

Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions

Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions

Yersiniose em Trachinotus marginatus (pampo): diagnóstico histopatológico e imuno-histoquímico

The Use of Kidney Biopsy of Broodstock Steelhead Trout (Oncorhyncus Mykiss) to Determine the Status of Bacterial Kidney Disease Infection

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