Detection of <i>Salmonella</i> enteritidis Using a Miniature Optical Surface Plasmon Resonance Biosensor

Son, Jiwoong; Kim, G; Kothapalli, Aparna; Morgan, Mark T.; Ess, Daniel R.

doi:10.1088/1742-6596/61/1/215

Cited by 29 publications

(20 citation statements)

References 11 publications

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“…While several researchers reported either an improvement of LOD [25], as similarly reported in this work, or improvement of the detection range with the transition from a direct into a sandwich format [26]. Other researchers, who used SPR for assaying bacterial pathogens, only report a detection in a direct format without reporting on a sandwich format, and all mentioned on the limited penetration depth of the evanescent wave as a problem for sensitive detection of bacterial pathogen in SPR-based assay methods [12,20]. Interestingly, in one report where the sandwich format gave a more sensitive LOD than a direct assay, the authors suggested that portion of the secondary antibody used can bind to the bacterial cells in areas that are within the penetration depth of the evanescent wavelengths especially near the area of the capture antibody.…”

Section: Sandwich Assaysupporting

confidence: 66%

“…LOD values of between 10 3 and 10 7 CFU·mL −1 are reported for SPR-based assays of pathogens, while LOD values of between 10 1 to 10 3 CFU·mL −1 are reported for QCMor piezoelectric-based assays, all in a direct format. For an instance, Son et al [20] reported a LOD value of 10 5 CFU mL −1 for detecting S. enteritidis in chicken samples, while Meeusen et al [21] reported a LOD value of 10 7 CFU mL -1 for both E. coli O157:H7 and S. Typhimurium [21]. A QCM-based direct detection method for Escherichia coli O157:H7 reported a LOD value of 10 cells [22], in a piezoelectric-excited millimeter-size cantilever (PEMC) while Salam et al [23], reported a direct detection of S. Typhimurium with a LOD value of 20 cells in a Sierra sensor QCM.…”

Section: Direct Assaymentioning

confidence: 99%

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Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Section: Sandwich Assaysupporting

confidence: 66%

Section: Direct Assaymentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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“…An optical surface plasmon resonance sensor using gold surface immobilized with anti-Salmonella antibody can detect Salmonella at 10 5 c.f.u./ml (Son et al 2007), while an enzyme-linked immunosorbent assay (ELISA) is capable of detecting 10 4 c.f.u./ml of Salmonella (Durant et al 1997), and. PCR-ELISA and LightCycler real-time PCR assays have a Salmonella detection limit of 10 3 c.f.u./ml (Perelle et al 2004).…”

Section: Combination Of Atp Assaysmentioning

confidence: 99%

Detection of living Salmonella cells using bioluminescence

et al. 2009

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ATP-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for Salmonella enteritidis detection. In this combination method, the Salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the ATP released from the cells was measured using mutant luciferase. This method resulted in approximately 1,000 times higher sensitivity in the detection of Salmonella (i.e. 10(3) c.f.u./ml) compared to immunochromatographic lateral flow assay.

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“…Biotinylated Salmonella antibodies were immobilized on a Neutravidin-coated gold sensor surface. Salmonella could be detected at concentrations as low as 10 6 cfu/mL in chicken extract [227]. [211] The BIACORE 3000 SPR sensor system [114] was established for L. monocytogenes in a subtractive inhibition assay [228].…”

Section: Amperometric Sensors Listeria Cells In Low-fat and Infantmentioning

confidence: 99%

Toxin immunosensors and sensor arrays for food quality control

Moises

Schäferling

2009

Bioanal Rev

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The global efforts to improve consumer protection and public health lead to an increasing number of analytical approaches applicable to food analysis and process control. Biosensor systems are efficient analytical tools to monitor production processes or storage of nutrition and to control contamination outbreaks as they are easy-touse, fast, and with minimal effort on sample preparation. Relevant targets of immunosensors implemented to food safety are prevalent bacterial toxins (staphylococcal enterotoxins and clostridial toxins), plant toxins (Ricin), mycotoxins (aflatoxins and ochratoxin A), marine toxins, and other pathogenic bacterial contaminations (Listeria, Salmonella, Staphylococcus aureus, or Escherichia coli). These cause acute intoxication and also chronic diseases in humans consuming contaminated food. Promising approaches for the determination of different types of toxins in food matrices will be outlined. The corresponding sensor systems use immunological receptor units such as antibodies or antigens and include optical (fluorescence and surface plasmon resonance), electrochemical, or acoustical readout methods. This review is focused on recent developments of sensor formats devoted to food safety control and is structured according to the type of toxin or contaminant that is recognized. It is intended to give an overview on emerging sensor technologies and their potential applications for the rapid analysis of the most important food poisoning agents.

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Detection of Salmonella enteritidis Using a Miniature Optical Surface Plasmon Resonance Biosensor

Cited by 29 publications

References 11 publications

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Detection of living Salmonella cells using bioluminescence

Toxin immunosensors and sensor arrays for food quality control

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