Detection of living Salmonella cells using bioluminescence

Urata, Masaaki; Iwata, Rei; Noda, Kenichi; Murakami, Yuji; Kuroda, Akio

doi:10.1007/s10529-009-9924-5

Cited by 18 publications

(3 citation statements)

References 10 publications

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“…We previously developed the higher catalytic mutant luciferase FM (Fujii et al 2007) and applied this enzyme for e cient assays for ATP as well as D-luciferin-generating enzymes using peptide-modi ed luciferin (Noda et al 2010;Noda et al 2008;Urata et al 2009). Here we found that luciferase CR showed higher luminescence than FM within various ATP concentrations in the presence of sodium chloride (Fig.…”

Section: Atp Assay Using Luciferase Crmentioning

confidence: 99%

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride

Yawata

Noda²,

Shimomura³

et al. 2021

Biotechnol Lett

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ObjectivesFire y luciferase, one of the most extensively studied enzymes, has numerous applications. However, luciferase activity is inhibited by sodium chloride. This study aims to expand the applications of re y luciferase in the presence of sodium chloride. ResultsWe rst obtained two mutant luciferase enzymes whose inhibition were alleviated and identi ed these mutations as Val288Ile and Glu488Val. Under dialysis condition (140 mM sodium chloride), the wild type was inhibited to 44% of its original activity level. In contrast, the single mutants, Val288Ile and Glu488Val, retained 67% and 79% of their original activity, respectively. Next, we introduced Val288Ile and Glu488Val mutations into the wild-type luciferase to create a double mutant using site-directed mutagenesis. Notably, the double mutant retained its activity more than 95% of that in the absence of sodium chloride. ConclusionsThe mutant luciferase, named luciferase CR, was found to retain its activity in various concentrations of sodium chloride. The inhibition of luciferase CR under dialysis condition was more alleviated than either Val288Ile or Glu488Val alone, suggesting that the effect of the double mutation was cumulative. We discussed the effect of mutations on the alleviation of the inhibition by sodium chloride.

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Section: Atp Assay Using Luciferase Crmentioning

confidence: 99%

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride

Yawata

Noda²,

Shimomura³

et al. 2021

Biotechnol Lett

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“…Lateral flow immunoassay (LFI) is a rapid, simple, and cost-effective method that has attracted growing interest from researchers and has been successfully used to detect pathogens (Urata et al, 2009;Ching et al, 2012;Xie et al, 2014;Chen et al, 2015a), toxins (Wang et al, 2011;Chen et al, 2013a;Ren et al, 2014), veterinary drugs (Song et al, 2013;Romero et al, 2014;Samsonova et al, 2015), heavy metals (Cho and Irudayaraj, 2013), and pesticides (Hossain et al, 2009;Shaker and Elsharkawy, 2015) in milk and milk powder. This review aims to describe recent developments in LFI for the detection of hazardous substances in milk and milk powder.…”

Section: Introductionmentioning

confidence: 99%

Invited review: Advancements in lateral flow immunoassays for screening hazardous substances in milk and milk powder

Chen

Huang

Song

et al. 2019

Journal of Dairy Science

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Dairy-related food safety outbreaks, such as foodborne pathogen contamination, mycotoxin contamination, and veterinary drug contamination, sometimes happen and have been reported all over the world, affecting human health and, in some cases, leading to death. Thus, rapid yet robust detection methods are needed to monitor milk and milk powder for the presence of hazardous substances. The lateral flow immunoassay (LFI) is widely used in onsite testing because of its rapidity, simplicity, and convenience. In this review, we describe some traditional LFI used to detect hazardous substances in milk and milk powder. Furthermore, we discuss recent advances in LFI that aim to improve sensitivity or detection efficiency. These advances include the use of novel label materials, development of signal amplification systems, design of multiplex detection systems, and the use of nucleic acid-based LFI.

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“…Many methods have been developed to detect S. enterica; these methods include polymerase chain reaction (PCR) (Hein, Flekna, Krassnig, & Wagner, 2006;Perelle et al, 2004;Riyaz-Ul-Hassan, Verma, & Qazi, 2013), enzyme-linked immunosorbent assay (ELISA) (Durant, Young, Nisbet, Stanker, & Ricke, 1997;Galikowska et al, 2011;Mansfield & Forsythe, 2000), gold lateral flow strip (GLFS) (Urata, Iwata, Noda, Murakami, & Kuroda, 2009), and surface plasmon resonance (SPR) (Son, Kim, Kothapalli, Morgan, & Ess, 2007). PCR is highly sensitive, but it requires precise and expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

Magnetic relaxation switch immunosensor for the rapid detection of the foodborne pathogen Salmonella enterica in milk samples

Wang

Zhang

et al. 2015

Food Control

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a b s t r a c tWe present a rapid and sensitive magnetic relaxation switch (MRS) immunosensor based on superparamagnetic nanoparticles (SMNs) for detecting the foodborne pathogen Salmonella enterica in milk samples. The MRS immunosensor employed target-induced aggregation (or disaggregation) of SMNs in immunoreaction, which could modulate the spinespin relaxation time of the neighboring water molecule. Results showed that the linear range of the MRS immunosensor for detecting S. enterica was 5 Â 10 3 e10 6 CFU/mL, and that the relative standard deviation was below 13.5%. The detection limit of the sensor was 10 3 CFU/mL, which was 20 times lower than that of enzyme-linked immunosorbent assay (ELISA, 2 Â 10 4 CFU/mL). The analysis time of the sensor (30 min) was also shorter than that of ELISA (2e4 h). This sensor was successfully applied to detect S. enterica in 20 real milk samples, and its accuracy was better than that of ELISA. Therefore, the MRS immunosensor provides a promising platform for the determination of foodborne pathogenic bacteria because of its high sensitivity, simplicity, rapidness, and low cost.

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Detection of living Salmonella cells using bioluminescence

Cited by 18 publications

References 10 publications

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride

Invited review: Advancements in lateral flow immunoassays for screening hazardous substances in milk and milk powder

Magnetic relaxation switch immunosensor for the rapid detection of the foodborne pathogen Salmonella enterica in milk samples

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