Detection of <i>Slackia exigua, Mogibacterium timidum</i> and <i>Eubacterium saphenum</i> from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method

Hashimura, T.; Sato, M.; Hoshino, Eiichi

doi:10.1046/j.1365-2591.2001.00419.x

Cited by 45 publications

(26 citation statements)

References 24 publications

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“…Surprisingly, S. exigua, which is not well studied, was found to be among the most abundant colonizers of all subjects at all time points, with a mean HOMIM score across time points of 1.9 (see Table S2 in the supplemental material). Typically associated with endodontic (49) and periodontal lesions (50), the species is fastidious and grows poorly (51) and may therefore have been easily overlooked in previous culture-based studies. The key colonizers identified here form the substratum for the developing biofilm architecture.…”

Section: Discussionmentioning

confidence: 99%

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Heller

Helmerhorst

Gower

et al. 2016

Appl Environ Microbiol

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f Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. The aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. Samples of early dental biofilm were collected from 11 healthy subjects at 0, 2, 4, and 6 h after removal of plaque and pellicle from tooth surfaces. With the semiquantitative Human Oral Microbiome Identification Microarray (HOMIM) technique, which is based on 16S rRNA sequence hybridizations, plaque samples were analyzed with the currently available 407 HOMIM microbial probes. This led to the identification of at least 92 species, with streptococci being the most abundant bacteria across all time points in all subjects. High-frequency detection was also made with Haemophilus parainfluenzae, Gemella haemolysans, Slackia exigua, and Rothia species. Abundance changes over time were noted for Streptococcus anginosus and Streptococcus intermedius (P ‫؍‬ 0.02), Streptococcus mitis bv. 2 (P ‫؍‬ 0.0002), Streptococcus oralis (P ‫؍‬ 0.0002), Streptococcus cluster I (P ‫؍‬ 0.003), G. haemolysans (P ‫؍‬ 0.0005), and Stenotrophomonas maltophilia (P ‫؍‬ 0.02). Among the currently uncultivable microbiota, eight phylotypes were detected in the early stages of biofilm formation, one belonging to the candidate bacterial division TM7, which has attracted attention due to its potential association with periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Heller

Helmerhorst

Gower

et al. 2016

Appl Environ Microbiol

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“…In recent years, molecular methods have also been used to investigate the microbiota associated with infected root canals and with acute periapical abscesses [2,[9][10][11][12][13][14][15][16][17][18][19][20]. These methods have provided significant additional knowledge regarding the composition of the endodontic microbiota.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, other fastidious bacterial species, such as Por. endodontalis [13,18], Slackia exigua [11], Mogibacterium timidum [11] and Eubacterium saphenum [11], have been reported in endodontic infections in higher prevalence values when molecular methods were used.…”

Section: Introductionmentioning

confidence: 99%

Comparison of 16S rDNA-based PCR and checkerboard DNA–DNA hybridisation for detection of selected endodontic pathogens

Siqueira

Rôças

Uzeda

et al. 2002

Journal of Medical Microbiology

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Molecular methods have been used recently to investigate the bacteria encountered in human endodontic infections. The aim of the present study was to compare the ability of a 16S rDNA-based PCR assay and checkerboard DNA-DNA hybridisation in detecting Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Peptostreptococcus micros, Porphyromonas endodontalis, Por. gingivalis and Treponema denticola directly from clinical samples. Specimens were obtained from 50 cases of endodontic infections and the presence of the target species was investigated by whole genomic DNA probes and checkerboard DNA-DNA hybridisation or taxon-specific oligonucleotides with PCR assay. Prevalence of the target species was based on data obtained by each method. The sensitivity and specificity of each molecular method was compared with the data generated by the other method as the reference -a value of 1.0 representing total agreement with the chosen standard. The methods were also compared with regard to the prevalence values for each target species. Regardless of the detection method used, T. denticola, Por. gingivalis, Por. endodontalis and B. forsythus were the most prevalent species. If the checkerboard data for these four species were used as the reference, PCR detection sensitivities ranged from 0.53 to 1.0, and specificities from 0.5 to 0.88, depending on the target bacterial species. When PCR data for the same species were used as the reference, the detection sensitivities for the checkerboard method ranged from 0.17 to 0.73, and specificities from 0.75 to 1.0. Accuracy values ranged from 0.6 to 0.74. On the whole, matching results between the two molecular methods ranged from 60% to 97.5%, depending on the target species. The major discrepancies between the methods comprised a number of PCR-positive but checkerboard-negative results. Significantly higher prevalence figures for Por. endodontalis and T. denticola were observed after PCR assessment. There was no further significant difference between the methods with regard to detection of the other target species.

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“…In recent years, there have been reports that Slackia exigua is a poorly growing pathogen in periodontitis and periapical infections (1,5,8,20,23,27). This bacterium was originally classified as Eubacterium exiguum in 1996 (20) and was reclassified as S. exigua in 1999 (27).…”

mentioning

confidence: 99%

“…exigua has frequently been isolated from periradicular lesions (8,13,22), in addition to other oral sites (9,18,28). In particular, S. exigua has been found in infected necrotic pulps and periradicular lesions (8,22).…”

mentioning

confidence: 99%

Characterization of Slackia exigua Isolated from Human Wound Infections, Including Abscesses of Intestinal Origin

Kim

Rowlinson²,

Bennion

et al. 2010

J Clin Microbiol

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Eleven clinical strains isolated from infected wound specimens were subjected to polyphasic taxonomic analysis. Sequence analysis of the 16S rRNA gene showed that all 11 strains were phylogenetically related to Slackia exigua. Additionally, conventional and biochemical tests of 6 of the 11 strains were performed as supplementary methods to obtain phenotypic identification by comparison with the phenotypes of the relevant type strains. S. exigua has been considered an oral bacterial species in the family Coriobacteriaceae. This organism is fastidious and grows poorly, so it may easily be overlooked. The 16S rRNA gene sequences and the biochemical characteristics of four of the S. exigua strains isolated for this study from various infections indicative of an intestinal source were almost identical to those of the validated S. exigua type strain from an oral source and two of the S. exigua strains from oral sources evaluated in this study. Thus, we show for the first time that S. exigua species can be isolated from extraoral infections as well as from oral infections. The profiles of susceptibility to selected antimicrobials of this species were also investigated for the first time.

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Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method

Abstract: S. exigua, M. timidum and E. saphenum were present in root canal systems, and may be associated with pulpal and periradicular pathosis.

Cited by 45 publications

References 24 publications

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Microbial Diversity in the Early In Vivo -Formed Dental Biofilm

Comparison of 16S rDNA-based PCR and checkerboard DNA–DNA hybridisation for detection of selected endodontic pathogens

Characterization of Slackia exigua Isolated from Human Wound Infections, Including Abscesses of Intestinal Origin

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