Detection of IgG Anti-Lewis (a) Antibodies in Cord Sera by
Kinetic Elisa

Spitalnik, S.; Cowles, J.; Cox, M.T.; Blumberg, N.

doi:10.1159/000465766

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…It is generally assumed that most Lewis antibodies are IgM. However, it has been determined that most sera containing Lewis antibodies have an IgG component and that, rarely, some are entirely IgG [135,138,139].…”

Section: Clinical Significance Of Lewis Antibodiesmentioning

confidence: 99%

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Lewis Histo-Blood Group System and Associated Secretory Phenotypes

Henry¹,

Oriol²,

Samuelson³

1995

Vox Sanguinis

127

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This review summarises present knowledge of the chemistry, immunology, genetics and clinical significance of antibodies in the Lewis and secretor histo- blood group systems. Although red cell serology has laid the foundations for these systems, more recent advances have been made by studying Lewis and related glycoconjugates with monoclonal antibodies, determining structures by mass spectrometry and NMR spectroscopy, identifying enzymes and their specificities, and identifying the genes by molecular biology. The expression of Lewis system antigens is dependent on Lewis and secretor loci. Fucosyltrans- ferases coded by genes at these loci compete and interact with each other and with other transferases to determine an individual’s Lewis and secretor phenotype. Exocrine epithelial cells, mostly of endodermal origin, synthesise the Lewis antigens which, as plasma glycolipids, are secondarily acquired by cells of the peripheral circulation. Phenotyping red cells is often regarded as a simple way of determining the Lewis and sometimes the secretor status of an individual; however, the red cell phenotype is influenced by many factors and may not necessarily reflect someone’s Lewis and secretor genotypes. Two main red cell Lewis groups are usually found, Lewis negative and Lewis positive. In Lewis-negative individuals, the secretor genotype does not affect the Lewis phenotype, but in Lewis-positive individuals, the non-secretor genotype generates the Le(a+b-) phenotype, the secretor genotype causes the Le(a-b+ ) phenotype, and the partial secretor genotype gives rise to the Le(a+b+) phenotype.

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Section: Clinical Significance Of Lewis Antibodiesmentioning

confidence: 99%

“…In contrast, subjects with the Le(a+b-) phenotype do not have Leb antigens and can make anti-Leb, albeit infrequently [135,[138][139][140]. Most examples of anti-Lea are formed by subjects of the Le(a-b-) ABH secretor phenotype, whereas most examples of anti-Leb are…”

Section: Clinical Significance Of Lewis Antibodiesmentioning

confidence: 99%

Lewis Histo-Blood Group System and Associated Secretory Phenotypes

Henry¹,

Oriol²,

Samuelson³

1995

Vox Sanguinis

127

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“…Nevertheless, Spitalnik et al [7] have demonstrated by means of a sensitive and specific enzymelinked immunosorbent assay that an IgG component occurred in all 13 maternal sera studied which were anti-Leacarriers, and that in 12 of the cases the antibody having crossed the placenta could be detected in cord serum samples. On the other hand, Longworth et al [8], using potent mouse monoclonal antiLewis grouping reagents, reported that 8% of cord blood samples reacted weakly with monoclonal, but not with polyclonal, antiLe".…”

mentioning

confidence: 99%

Mild Hemolytic Disease of the Newborn due to Anti-Lewis^a

Vescio¹,

Torres²,

Virgilio³

et al. 1993

Vox Sanguinis

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Lewis blood group system review

Combs

2009

Immunohematology

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The expression of Lewis blood group antigens depends on the alleles inherited at two independent loci, FUT2 (Secretor [SE ] gene) and FUT3 (Lewis [LE ] gene). The Se and Le alleles encode separate fucosyltransferases that interact to form Lewis antigens in secretions and fluids. The Lewis antigens on RBCs are not integral to the membrane but are passively adsorbed from the plasma. The antigens are widely distributed in human tissue and fluids and are receptors for some pathogenic bacteria. Lewis antibodies are rarely clinically significant, although there are rare reports of hemolytic transfusion reactions, hemolytic disease of the fetus and newborn, and renal transplant rejection. This review provides a general overview of the Lewis blood group system. An extensive overview by Daniels1 contains additional detailed information on the Lewis blood group system and related antigens.

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Detection of IgG Anti-Lewis (a) Antibodies in Cord Sera by Kinetic Elisa

Cited by 3 publications

References 7 publications

Lewis Histo-Blood Group System and Associated Secretory Phenotypes

Lewis Histo-Blood Group System and Associated Secretory Phenotypes

Mild Hemolytic Disease of the Newborn due to Anti-Lewis^a

Lewis blood group system review

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