Detection of immunoglobulin antibodies in the sera of patients using purified latex allergens

Vp, Kurup; Hy, Yeang; Gl, Sussman; Nk, Bansal; Beezhold, Donald H.; Kelly, Kevin J.; Dr, Hoffman; Williams, B. D.; Jn, Fink

doi:10.1046/j.1365-2222.2000.00748.x

Cited by 58 publications

(54 citation statements)

References 34 publications

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“…In this study, 81% of the SB patients and 50% of the HCWs showed IgE reactivity to Hev b 1 [18]. More recent studies performed with natural and recombinant Hev b 1 clearly demonstrated that Hev b 1 was one of the major allergens of Hevea latex for SB patients with prevalences ranging from 54 to 100%, but that it was less important for HCWs and other latex-allergic individuals with prevalences ranging from 13 to 32% [19,20,21]. …”

Section: Hev B 1 (Rubber Elongation Factor)mentioning

confidence: 74%

“…Natural and recombinant Hev b 3 proteins were used in several studies revealing similar prevalences. For SB patients, prevalences ranged from 77 to 100% and from 7 to 32% for HCWs and latex-allergic individuals [19,20,21, 25]. Interestingly, prevalence data of Hev b 3 highly correlate with the data obtained for Hev b 1, indicating that these proteins contain very similar IgE epitopes.…”

Section: Hev Bmentioning

confidence: 88%

“…The cDNA of Hev b 2 codes for a protein of 374 amino acid residues with N-terminal and C-terminal extensions that are cleaved off, resulting in a mature protein of 35.1 kDa with one putative N-glycosylation site [23]. Hev b 2 seems to be a major allergen of Hevea latex with prevalences ranging from 38 to 65% [19, 21]. Yagami et al [24] described that the carbohydrate structures of Hev b 2 were the major IgE-binding epitopes.…”

Section: Hev B 2 (β-13-glucanase)mentioning

confidence: 99%

“…Studies with natural Hev b 4 have demonstrated that this protein was an important allergen for HCWs as well as SB patients. Kurup et al [19] performed ELISA and RAST studies with nHev b 4. The prevalences obtained with RAST assays were lower than those obtained with ELISAs.…”

Section: Hev B 4 (Cyanogenic Glucosidase)mentioning

confidence: 99%

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<i>Hevea brasiliensis</i> Latex Allergens: Current Panel and Clinical Relevance

Wagner

Breiteneder²

2005

Int Arch Allergy Immunol

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Latex allergy has been studied in detail in Europe and the US over the past two decades, resulting in specific guidelines that succeeded in reducing its incidence in high-risk populations within the medical field. How these developments have affected high-risk populations outside the health care scenario is an important unanswered question. In addition, a second wave of latex allergy may occur in nations that are striving to attain higher economic and technologic standards, including population-dense countries such as China. Therefore, the application of Hevea allergens in novel diagnostic assays and the development of specific latex immunotherapy will provide new opportunities for latex allergy research. In this review, we summarize current knowledge on the immunological properties of the 13 officially accepted Hevea brasiliensis latex allergens.

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Section: Hev B 1 (Rubber Elongation Factor)mentioning

confidence: 74%

Section: Hev Bmentioning

confidence: 88%

Section: Hev B 2 (β-13-glucanase)mentioning

confidence: 99%

Section: Hev B 4 (Cyanogenic Glucosidase)mentioning

confidence: 99%

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<i>Hevea brasiliensis</i> Latex Allergens: Current Panel and Clinical Relevance

Wagner

Breiteneder²

2005

Int Arch Allergy Immunol

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“…Section 1734 solely to indicate this fact. (10,16,(17)(18)(19)(20), depending on the analytical sensitivity of the serologic tests employed. On the other hand, the occurrence of IgE reactivity to the 42-to 46-kDa protein detected in unpurified whole latex was commonly above 60% (median 71%) (6,9,19).…”

mentioning

confidence: 99%

Isolation and Characterization of the Early Nodule-specific Protein Homologue (Hev b 13), an Allergenic Lipolytic Esterase from Hevea brasiliensis Latex

Arif

Hamilton

Yusof

et al. 2004

Journal of Biological Chemistry

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Recurring reports of a highly allergenic 42-46-kDa protein in Hevea brasiliensis latex appeared to have been resolved with the discovery of a 43-kDa allergenic latex protein that was a homologue to patatin. However, the low to moderate prevalence of sensitization to the protein, designated Hev b 7, among latex-allergic patients could not adequately explain the frequent observations of the 42-46-kDa allergen. This led to the hypothesis that another, more allergenic protein of a similar molecular mass existed in Hevea latex. We report the isolation and purification of a 42.98-kDa latex glycoprotein showing homology to the early nodule-specific protein (ENSP) of the legumes Medicago sativa, Medicago truncatula, and Glycine max. The protein is allergenic, being recognized by immunoglobulin E (IgE) in sera from latex-allergic patients. The IgE epitope resides on the carbohydrate moiety of the protein, and the presence of a similar carbohydrate component on potato tuber patatin enables the latter to inhibit IgE binding to the ENSP homologue. The cDNA encoding the ENSP homologue was isolated by reverse transcription-PCR and cloned. The protein predicted from the cDNA sequence has 391 amino acids, the first 26 of which constitute a putative signal peptide. The deduced molecular mass of the mature protein is 40.40 kDa, while its isoelectric point is estimated at 5.0. The discrepancy between the predicted and observed molecular mass might be due to glycosylation, for which three N-sites on the protein are predicted. The purified protein showed lipase and esterase activities and may be involved in plant defense.Latex exudes from the bark of the commercial rubber tree, Hevea brasiliensis, when the tree is tapped. A large proportion of the harvested latex is coagulated for manufacture into "dry rubber" industrial products such as motor vehicle tires. Latex can be stabilized in an uncoagulated state by ammoniation for subsequent processing into various "dipped" latex products such as examination and surgical gloves. About one-third by weight of Hevea latex is natural rubber, the polymeric hydrocarbon cis-polyisoprene that is synthesized in specialized organelles, the rubber particles. Another specialized organelle found in latex is the lutoid, which is vacuole-like in structure and function. In fresh latex, rubber particles, lutoids, and other minor cellular organelles are suspended in an aqueous serum called the C-serum. Hevea latex contains hundreds of proteins, making up 1-2% of its weight (1, 2). Among these, a small number of latex proteins can cause an allergenic reaction in sensitized persons, particularly health care workers who routinely wear latex gloves in the course of their work. Many latex allergens originate from the B-serum (3), which is the fluid derived from the heavy "bottom fraction" of centrifuged latex. The bottom fraction comprises mainly lutoids, but other minor organelles are also deposited in the bottom fraction when latex is centrifuged at high speed (4, 5).Much effort has focused on the isolation a...

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