Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues

McGuire, Sean E.; Hilsenbeck, Susan G.; Figueroa, JoséA A.; Jackson, James G.; Yee, Douglas

doi:10.1016/0304-3835(94)90343-3

Cited by 34 publications

(25 citation statements)

References 21 publications

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“…Early studies identified increased IGFBP-5 expression in tumours and led to the proposal that IGFBP-5 was pro-metastatic (Hou, Zhang, 2009, Huynh, 1998, McGuire, Hilsenbeck, 1994, Mita, Zhang, 2007, Pekonen, Nyman, 1992. However, our data, along with other recent reports, suggest that IGFBP-5 is protective.…”

Section: Igfbp-5 and Cancermentioning

confidence: 99%

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IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion

Vijayan

Guha

Ameer

et al. 2013

The International Journal of Biochemistry & Cell Biology

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SummaryTGFβ1 is a major fibrotic factor and its actions involve induction of epithelial cell death, together with the stimulation and transdifferentiation of fibroblasts into collagen-and fibronectin-secreting myofibroblasts. These actions of TGFβ1 are also consistent with a pro-metastatic role, by aiding epithelial cell escape through mesenchymal tissues. Recently IGFBP-5 has been described as a pro-fibrotic (prometastatic?) agent and the aim of this study was to compare and contrast the actions of IGFBP-5 with TGFβ1. We used NMuMG cells and cloned stable epithelial and mesenchymal lines from the parent cells. TGFβ1 induced apoptosis and/or EMT in the epithelial cells, whereas it enhanced mesenchymal cell survival and migration.IGFBP-5, in contrast, enhanced both cell-cell and cell-ECM adhesion and also improved wound closure in epithelial cells whereas, in mesenchymal cells, IGFBP-5 decreased adhesion and migration. Furthermore, IGFBP-5 was able to antagonise the actions of TGFβ1. In a co-culture model simulating epithelial-mesenchymal boundaries, IGFBP-5 was able to antagonise the disruptive transgressions induced by TGFβ1. Overall, these findings suggest that IGFBP-5 is important in maintaining epithelial-mesenchymal boundaries and thus may limit metastasis and fibrosis by inducing an orderly repair mechanism, very distinct from the fibrotic disruption induced by TGFβ1. A role for IGFBP-5 in the inhibition of metastasis is supported by immunohistochemical studies of breast cancer microarrays, where we show that elevated IGFBP-5 expression is associated with increased disease-free survival.

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Section: Igfbp-5 and Cancermentioning

confidence: 99%

“…Paradoxically, increased IGFBP-5 expression has been associated with poor prognosis during metastasis (Hou et al , 2009, Huynh, 1998, McGuire et al , 1994, Mita et al , 2007, Pekonen et al , 1992.…”

Section: Introductionmentioning

confidence: 99%

IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion

Vijayan

Guha

Ameer

et al. 2013

The International Journal of Biochemistry & Cell Biology

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“…Studies on breast cancer specimens show a negative correlation between IGFBP3 expression and oestrogen receptor expression (McGuire et al, 1994;Yee, 1994;Yu et al, 1995). In addition, multiple studies show a positive correlation between IGFR and ER expression (Pekonen et al, 1988;Peyrat et al, 1988;Foekens et al, 1989;Papa et al, 1993;Railo et al, 1994).…”

Section: Interaction Between Igf and Oestrogen Receptorsmentioning

confidence: 99%

Breast cancer: further metabolic-endocrine risk markers?

1997

Br J Cancer

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Summary There is evidence that increased oestrogen receptor (ER) expression in normal mammary epithelium may be a risk marker for the development of breast cancer. Insulin-like growth factor 1 (IGF1) is a potent inducer of mitosis and has been shown to synergize with oestrogen in stimulating the growth of human breast cancer in vitro. In these cells oestradiol has been shown to upregulate IGF Khan et al, 1994). ER expression in normal breast tissue is correlated with its proliferative activity and it has been postulated that the mitogenic activity of insulin-like growth factor 1 (IGF1) might interact with that of oestradiol in promoting breast cancer development after the initiation of carcinogenesis (Clarke et al, 1997). It is useful to summarize recent studies attempting to correlate measurements of ER and IGF1 with those of IGFI receptor (IGFR) and binding proteins (IGFBPs) in human breast cancer and in normal mammary epithelium. IGF1, hyperinsulinaemia and breast cancerA considerable literature has shown that insulin-like growth factors IGF1 and IGF1 1 can stimulate the growth of human breast cancer cell lines in vitro (summarized in Westley and May, 1994).The mitogenic effects of both IGF1 and IGF1 1 are thought to be mediated mainly by their binding to the IGF1 receptor, which is located mainly in the epithelial component of breast cancer (Ellis et al, 1994). Both IGFs are expressed in the stromal component suggesting that they mainly exert a paracrine effect on the epithelium (Singer et al, 1995) but in addition, circulating IGF1 may exert an endocrine effect on breast tissue.It is relevant that hyperinsulinaemia is associated with raised levels of IGF1 and in addition, insulin levels determine the

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“…There are numerous studies on the role of IGFBP5 in survival and apoptosis of both normal and cancer cells. Recent studies have linked insulin-like growth factor (IGF)-binding protein 5 (IGFBP-5) to metastasis by revealing increased IGFBP-5 expression in metastatic patients (Pekonen et al, 1992;McGuire et al, 1994;Huynh, 1998;Mita et al 2007). One study showed IGFBP5 expression is inversely related with tumorogenesis of breast cancer (Polanco et al, 2010), where in another study, IGFBP-5 is shown to be associated with increased survival of breast cancer patients (Ahn et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of PIK3CA Gene Mutations with HRM Analysis and Association with IGFBP-5 Expression Levels in Breast Cancer

Dirican¹,

Kaya²,

Güllü³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues

Cited by 34 publications

References 21 publications

IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion

IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion

Breast cancer: further metabolic-endocrine risk markers?

Detection of PIK3CA Gene Mutations with HRM Analysis and Association with IGFBP-5 Expression Levels in Breast Cancer

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