Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines

McLean, S M; Mathew, M.; Kelly, Jason; Murray, Sally; Bennett, Harry; Webb, Lennox A.; Esakowitz, L; McLean, John S.

doi:10.1136/bjo.2005.071886

Cited by 24 publications

(12 citation statements)

References 17 publications

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“…Lens epithelial cell adhesion to BL is dependent on integrin‐mediated cell‐ECM adhesion (McLean et al. ). Integrins are heterodimeric transmembrane glycoproteins, consisting of one α and one β subunit.…”

Section: Discussionmentioning

confidence: 99%

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Anterior lens epithelial cells attachment to the basal lamina

Andjelić

Drašlar

Hvala

et al. 2015

Acta Ophthalmologica

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ABSTRACT.Purpose: To study the structure of the anterior lens epithelial cells (aLECs) and the contacts of the aLECs with the basal lamina (BL) in order to understand their role in the lens epithelium's function. Methods: The aLCs (BL and associated aLECs) were obtained from routine uneventful cataract surgery, prepared for and studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal microscopy. Results: SEM shows that the basal surface of the aLECs (~10-15 lm) is with aLECs foldings (~1-3 lm) and extensions (~0.5-3 lm) attached to the BL. Confocal microscopy images of the basal sections of the aLECs after membrane staining also suggest that the basal part of aLECs has foldings (~1-3 lm). TEM shows in the aLECs basal parts, towards BL, the structures that look like entanglement (~1-4 lm). In cases where there is a swelling of the cytoplasm and offset of the aLECs from the BL, individual extensions (~0.5-2 lm) that extend to the BL are visible by TEM. Conclusions: We provide detail evidence about the structural organization of the aLECs, in particular about their basal side which is in contact with the BL. This is supported by the complementary use of three techniques, SEM, TEM and confocal microscopy, each of them showing the same morphological features, the extensions and the entanglements of the aLECs cytoplasmic membrane at the border with the BL. The basal surface of the aLECs is increased. It suggests the functional importance of the contact between aLECs and BL.

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Section: Discussionmentioning

confidence: 99%

“…Integrins of two different subfamilies ( α 2, α 3, α 5 and β 2) are present in aLEC (McLean et al. ). The α 2 and α 3 integrin subunits are known to bind collagen type IV and laminin, respectively, and both of these ECM molecules are components of the BL.…”

Section: Discussionmentioning

confidence: 99%

Anterior lens epithelial cells attachment to the basal lamina

Andjelić

Drašlar

Hvala

et al. 2015

Acta Ophthalmologica

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“…Integrin a2b1 is a receptor for laminin, collage, fibronectin and Ecadherins. Whereas, a3b1 integrin can bind to matrix proteins such as collagen type IV and laminin that are major component of the lens capsule (McLean et al, 2005). This gives confidence to the use of SRA01/04 cell line as a model to study PCO in vitro.…”

Section: Cell Linesmentioning

confidence: 98%

Experimental models for posterior capsule opacification research

Wormstone

Eldred

2016

Experimental Eye Research

104

102

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Millions of people worldwide are blinded due to cataract formation. At present the only means of treating a cataract is through surgical intervention. A modern cataract operation involves the creation of an opening in the anterior lens capsule to allow access to the fibre cells, which are then removed. This leaves in place a capsular bag that comprises the remaining anterior capsule and the entire posterior capsule. In most cases, an intraocular lens is implanted into the capsular bag during surgery. This procedure initially generates good visual restoration, but unfortunately, residual lens epithelial cells undergo a wound-healing response invoked by surgery, which in time commonly results in a secondary loss of vision. This condition is known as posterior capsule opacification (PCO) and exhibits classical features of fibrosis, including hyperproliferation, migration, matrix deposition, matrix contraction and transdifferentiation into myofibroblasts. These changes alone can cause visual deterioration, but in a significant number of cases, fibre differentiation is also observed, which gives rise to Soemmering's ring and Elschnig's pearl formation. Elucidating the regulatory factors that govern these events is fundamental in the drive to develop future strategies to prevent or delay visual deterioration resulting from PCO. A range of experimental platforms are available for the study of PCO that range from in vivo animal models to in vitro human cell and tissue culture models. In the current review, we will highlight some of the experimental models used in PCO research and provide examples of key findings that have resulted from these approaches.

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“…Whether the results obtained in the rabbit model can be directly transferred to humans is unknown. Previous studies 24,25 have found differences at the molecular level between human LECs and rabbit LECs in terms of their interaction with modified surfaces.…”

Section: Discussionmentioning

confidence: 98%