Detection of intraoperative tumor cell dissemination in patients with breast cancer by use of reverse transcription and polymerase chain reaction

Brown, Douglas; Purushotham, Arnie; Birnie, G.D.; George, W.D.

doi:10.1016/s0039-6060(05)80235-1

Cited by 104 publications

(61 citation statements)

References 15 publications

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“…The first 5 ml was discarded to reduce contamination by epithelial cells at the site of needle entry. A further 10 ml was then collected in potassium ethylenediaminetetraacetic acid (EDTA) bottles and promptly transported to the laboratory at 4°C for immediate processing, as described by Brown et al (1995).…”

Section: Methodsmentioning

confidence: 99%

“…Several workers have attempted to identify circulating tumour cells in various malignancies (Smith et al, 1991;Burchill et al, 1994;Brown et al, 1995;Burchill et al, 1995;McCulloch et al, 1995;Choy and McCulloch, 1996). Various techniques, including morphology, flow cytometry and cytogenetics, have been used to detect disseminated tumour cells, but most of these have limited sensitivity and specificity (Frank et al, 1990;Diel et al, 1992;Johnson et al, 1995) and are time-consuming (Gross et al, 1995).…”

mentioning

confidence: 99%

“…Tumour-specific DNA sequence abnormalities have been identified mainly in haematological malignancies, such as acute lymphoblastic leukaemia, for which this technique has been used in the diagnosis and follow-up of patients (Yamada et al, 1990;Steward et al, 1994). For solid tumours, however, tumour-specific DNA sequence abnormalities are uncommon and, consequently, putative tissue-specific mRNAs have been used as molecular markers to detect circulating tumour cells by RT-PCR (Smith et al, 1991;Burchill et al, 1994;Brown et al, 1995;Eschwege et al, 1995). The K-ras gene mutation has been used to detect circulating colorectal cancer cells by an immunobead-PCR assay, but this approach is limited because of the low frequency of the gene mutations (30%) in these tumours .…”

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confidence: 99%

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Putative markers for the detection of breast carcinoma cells in blood

El-Tahir

Mallinson²,

Birnie³

et al. 1998

Br J Cancer

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Summary The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin 19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1 and CD44 (exons 5-11) were detected in normal peripheral blood, rendering these genes non-specific for epithelial cells and therefore unsuitable for use as markers to detect breast carcinoma cells. Although CK1 9 mRNA was apparently specific, it was deemed unsuitable for use as a marker of breast cancer cells in light of its limited sensitivity. Furthermore, an attempt at using nested primers to increase sensitivity resulted in CK19 mRNA being detected after two amplification rounds in blood from healthy volunteers.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Putative markers for the detection of breast carcinoma cells in blood

El-Tahir

Mallinson²,

Birnie³

et al. 1998

Br J Cancer

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“…The second problem is how to determine the appropriate extent of lymph node dissection in cases where SNs do contain metastases. Several investigators have reported using ultrasensitive molecular techniques for RT -PCR detection of micrometastasis in blood, bone marrow, and lymph nodes (Rosen et al, 1981;Ioguchi et al, 1994;Brown et al, 1995;Mori et al, 1995;Bostick et al, 1998;Takakura et al, 1998). This method has increased the detection rate for occult metastases as a result of amplification of mRNA tumour markers or epithelial markers and it has been suggested that patients exhibiting a positive band for these mRNAs in lymph nodes had a significantly worse prognosis than those who were RT -PCR-negative (Natsugoe et al, 1998;Cai et al, 1999;Noura et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Micrometastases in sentinel nodes of gastric cancer

Ajisaka

Miwa

2003

Br J Cancer

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The sentinel node (SN) is the first lymph node in the lymphatic basin to be affected by metastasis from the primary tumour and is used to predict the status of the remaining nodes in the basin. We succeeded in detecting SNs of clinically early gastric cancers by intraoperative injection of a blue dye around the tumour. In the study presented here, multiple-marker reverse transcriptionpolymerase chain reaction (RT -PCR) was used to detect micrometastases in SNs and results were compared with those obtained with conventional histology. Expressions of cytokeratin-18 (CK-18), carcinoembryonic antigen (CEA), human telomerase reverse transcriptase (hTRT) and MUC-1 in SNs were determined by RT -PCR and Southern blot assay. Of the 213 SNs obtained from 35 cases of gastric cancer, eight nodes (3.8%) from five patients contained metastases that could be identified by conventional histology. However, CK-18 mRNA was expressed in 15 (7.0%), CEA in 12 (5.6%), hTRT in 10 (4.7%), and MUC-1 in 12 (5.6%) nodes, with at least one mRNA marker expressed in 25 nodes (11.7%) obtained from six patients. In the five patients with nodal metastases identified by conventional histology, two had metastases in both SNs and non-SNs. And, in the 30 patients without nodal metastases identified by conventional histology, one patient with micrometastases in the SNs identified by RT -PCR and Southern blot assay also had metastases in non-SNs as identified by serial sectioning and immunostaining of CK-18. All additional metastases were detected in non-SNs located in the same lymphatic basin as the previously detected SNs. This suggests that lymph node dissection of early-stage gastric cancer in the lymphatic basin may be mandatory even for patients without histologically detectable metastases in SNs. British Journal of Cancer (2003) Lymph node metastasis is one of the most important determinants of whether minimally invasive surgery for early gastric cancer is appropriate, since lymph node dissection generally is considered unnecessary for early gastric cancer without nodal metastasis. Endoscopic mucosal resection or minimally invasive surgery without lymphadenectomy has recently been advocated for early gastric cancer without nodal metastasis. Predicting the absence of nodal metastasis is usually based on indirect evidence, by comparing the primary tumour in a particular case with those in other cases (Makuuchi et al, 1999). We succeeded in identifying sentinel nodes (SNs) in gastric cancer by intraoperative injection of blue dye around the tumour (Miwa et al, 2003). The SN is the first lymph node in the lymphatic basin to be affected by metastasis from the primary tumour and is used to predict the status of lymph nodes in the rest of the lymphatic basin (Morton et al, 1992). Metastases in the SNs are determined by intraoperative pathology, but if there is a discrepancy between the intraoperative diagnosis and postoperative diagnosis detailed examination results, a definitive operation cannot be performed only on the basis of a sentinel node biop...

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“…A meta-analysis of five trials using the suboptimal CMF regimen administered within 72 h after surgery has demonstrated an absolute benefit of 3.4% in terms of disease-free survival for patients treated within this delay (Clahsen et al, 1997). Moreover, another fundamental data that has been omitted by the authors are the release of circulating cells by tumour removal (Brown et al, 1995). In this line, we also might address the question of the possible release of circulating cells during biopsy, which could lead to a considerable increase in the delay to systemic therapy.…”

Section: Sirmentioning

confidence: 99%

Does timing of adjuvant chemotherapy influence the prognosis after early breast cancer?

Alliot¹

2006

Br J Cancer

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Detection of intraoperative tumor cell dissemination in patients with breast cancer by use of reverse transcription and polymerase chain reaction

Cited by 104 publications

References 15 publications

Putative markers for the detection of breast carcinoma cells in blood

Putative markers for the detection of breast carcinoma cells in blood

Micrometastases in sentinel nodes of gastric cancer

Does timing of adjuvant chemotherapy influence the prognosis after early breast cancer?

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