Detection of Intraspecific DNA Content Variation in<i>Zea mays</i>L. by Flow Cytometry

Rayburn, A. Lane; Auger, Julie; Benzinger, Elizabeth A.; Hepburn, Angus G.

doi:10.1093/jxb/40.11.1179

Cited by 122 publications

(59 citation statements)

References 14 publications

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“…Other researchers have suggested that DAPI is less affected by chromatin condensation than other DNA dyes (Shapiro, 1995). Rayburn et al (1989) observed that in maize nuclear DNA amounts obtained by flow cytometry using the dye DAPI gave similar results to nuclei stained with Schiff's reagent and analyzed by microdensitometry. Important to these findings is that the maize lines used in the study differed substantially in the amount of heterochromatin present.…”

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confidence: 80%

Metamorphosis inXenopus laevisis not associated with large-scale nuclear DNA content variation

Freeman

Rayburn

2004

Journal of Experimental Biology

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presence of nuclei of variable fluorescence intensity within the unfixed nuclei. Upon optimum fixation, which has been speculated to result in more homogeneous chromatin conformation and to reduce staining artifacts, the nuclei were observed to have less fluorescence intensity variation. The differential fluorescence observed in this study is consistent with the hypothesis that large-scale intraindividual DNA variation is not associated with amphibian metamorphosis.

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confidence: 80%

Metamorphosis inXenopus laevisis not associated with large-scale nuclear DNA content variation

Freeman

Rayburn

2004

Journal of Experimental Biology

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“…These reach a certain level and become stabilized during micro-evolution and gradual selection (Price et al 1980). Such interspecific variation in DNA amount is not exceptional in angiosperm species (Rayburn et al 1989).…”

Section: Discussionmentioning

confidence: 98%

Study of Karyotype Variability and Genome Size in 13 Species of <i>Cassia</i> L. in Interpreting Interspecific Genetic Diversity

Mohanty

Das

2006

CYTOLOGIA

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Summary Somatic chromosome number, karyotype and 4C DNA content was studied in 13 species of Cassia of the family Leguminosae obtained from Royal Botanic Gardens, Kew. Somatic chromosome number 2nϭ28 was observed in all the species except C. lindheimariana and C. versicolor with 2nϭ26 chromosomes. In addition, polyploid chromosomes were observed in C. atomaria and C. hirsuta (2nϭ56). Variations among the species were observed in their minute structural details of the karyotype. On the basis of centromeric index, 4 types (Type A, B, C and D) of chromosomes were found in different species. Significant variations in the chromosome length were observed among different species from 13.25 mm in C. wislizenii to 24.73 mm in C. polyantha. Total chromosome volume varied significantly from 6.93 mm 3 in C. hirsuta to 11.10 mm 3 in C. lindheimariana. Highest centromeric index percentage (43.69%) was noted in C. italica and that of lowest (34.12%) was found in C. polyantha. Significant variation in 4C nuclear DNA content was observed among the species that varied from 6.45 pg in C. italica to 15.77 pg in C. spectabilis. The correlation coefficients showed various chromosomal and nuclear parameters interdependent suggesting a interspecific relationship between structural and genetic changes of the genome architecture during evolution of speciation.

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“…The diversity of DNA amount has often been attributed to loss or addition of highly repetitive DNA sequences rather than the AT-or GC-reach sequences in a genome (Martel et al 1997) which after a certain level got stabilized during micro-evolution and gradual selection (Price et al 1980). Such interspecific variations in DNA amount were not exceptional in angiosperm species (Laurie and Bennett 1985, Rayburn et al 1989, Tito et al 1991. Further studies on the phylogeny of the Typhonium using different DNA markers might help in understanding the evolution of these taxa and the genomic in situ hybridization might provide further clues to the evolutionary paths and the ancestry for the adaptive characteristics of the genus Typhonium.…”

Section: Nuclear Dna Analysis Genome Size and Invmentioning

confidence: 99%

Variation in Chromosome Number, Karyotype and Nuclear DNA Content of Five Species of Indian Typhonium Schott. (Araceae)-A Medicinally Important Plant

et al. 2006

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Summary Detailed karyotype analysis of somatic chromosomes in 5 species of Typhonium Schott. of the family Araceae was carried out for the first time. The chromosome number varied from 2nϭ16 in T. flagelliforme to 2nϭ52 in T. diversifollium. Chromosome number 2nϭ18 and 2nϭ26 in T. trilobatum and T. roxburghii respectively suggest formation of anueploids during speciation. The asymmetric karyotype revealed structural alterations with 2 distinct types of chromosomes (long and very short types) as evident in different species of Typhonium. Significant variations in chromosome size were observed among the studied species. TF% revealed sub-metacentric long chromosomes in T. flagelliforme (TF value, 33%) and metacentric short chromosomes in T. diversifolium (TF value, 41%). Average chromosome length varied from 2.03 mm in T. diversifolium, a high altitude species (5000-7000 ft), to 8.68 mm in T. flagelliforme, a low land species (70-250 ft). Significant variations were observed in Interphase Nuclear Volume (INV) which varied from 142.05 mm 3 in T. trilobatum to 335.02 mm 3 in T. flagelliforme; nuclear DNA content varied significantly from 0.199 pg in T. diversifolium to 0.833 pg in T. flagelliforme.Key words Chromosome number, Karyotype, Nuclear DNA, Typhonium.The genus Typhonium Schott. of the family Araceae comprises about 79 species distributed mostly in the south-east Asia and north-east Australia (Hay 1993). In India and Indo-Malesian region, the genus is represented by 16 species (Santapau and Henry 1973). While Typhonium divaricatum (Linn.) Decne occurs mainly in the Penninsular India, T. trilobatum (Linn.) Schott. and T. flagelliforme Decne are the commonly found species in Bengal, Bihar, Orissa and Coromandel coast.In T. trilobatum, somatic chromosomes 2nϭ18 was reported (Sharma and Mukhopadhyay 1965) and 2nϭ26 (Simmonds 1954) were reported with distinctly different morphological characteristics among the ecotypes. Taxonomical grouping of some members of Araceae i.e. genus Sauromatum and Lazarum into Typhonium (Hay 1993) was of interest to us. However such groups based on chromosomal parameters, their karyotypes are not available. This paper deals with detailed investigations on chromosome number their karyotype, interphase nuclear volume (INV) and in situ nuclear DNA content in 5 Indian species of Typhonium may provide insight into chromosome behavior and genomic characteristics during the evolution and speciation in this genus. Materials and methods PlantsTubers of 5 species of Typhonium were collected from varied ecological conditions and main-

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Detection of Intraspecific DNA Content Variation inZea maysL. by Flow Cytometry

Cited by 122 publications

References 14 publications

Metamorphosis inXenopus laevisis not associated with large-scale nuclear DNA content variation

Metamorphosis inXenopus laevisis not associated with large-scale nuclear DNA content variation

Study of Karyotype Variability and Genome Size in 13 Species of <i>Cassia</i> L. in Interpreting Interspecific Genetic Diversity

Variation in Chromosome Number, Karyotype and Nuclear DNA Content of Five Species of Indian Typhonium Schott. (Araceae)-A Medicinally Important Plant

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