Detection of iron-labeled single cells by MR imaging based on intermolecular double quantum coherences at 14 T

Cho, Jee Hyun; Hong, Kwan Soo; Cho, Janggeun; Chang, Sun Ju; Cheong, Chaejoon; Lee, Na Hee; Kim, Hyeonjin; Warren, Warren S.; Ahn, Sangdoo; Lee, Chul-Hyun

doi:10.1016/j.jmr.2012.02.014

Cited by 4 publications

(3 citation statements)

References 42 publications

(52 reference statements)

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“…This image modality possesses high spatial resolution, which facilitates long-term and single-cell detection, and is noninvasive and utilizes nonionizing radiation. Cells labeled with SPIO exhibit low-intensity signals during T2 and T2 ∗ MRI imaging [ 113 , 115 ]. MION labels stem cells without requiring the use of a transfection agent [ 116 ] and does not affect cell viability, phenotype, and in vitro differentiation capacity [ 112 ].…”

Section: In Vivo Imaging Systems and Tracker Agmentioning

confidence: 99%

Potential Therapeutic Mechanisms and Tracking of Transplanted Stem Cells: Implications for Stroke Treatment

Zhang

Yao

2017

Stem Cells International

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Stem cell therapy is a promising potential therapeutic strategy to treat cerebral ischemia in preclinical and clinical trials. Currently proposed treatments for stroke employing stem cells include the replacement of lost neurons and integration into the existing host circuitry, the release of growth factors to support and promote endogenous repair processes, and the secretion of extracellular vesicles containing proteins, noncoding RNA, or DNA to regulate gene expression in recipient cells and achieve immunomodulation. Progress has been made to elucidate the precise mechanisms underlying stem cell therapy and the homing, migration, distribution, and differentiation of transplanted stem cells in vivo using various imaging modalities. Noninvasive and safe tracer agents with high sensitivity and image resolution must be combined with long-term monitoring using imaging technology to determine the optimal therapy for stroke in terms of administration route, dosage, and timing. This review discusses potential therapeutic mechanisms of stem cell transplantation for the treatment of stroke and the limitations of current therapies. Methods to label transplanted cells and existing imaging systems for stem cell labeling and in vivo tracking will also be discussed.

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Section: In Vivo Imaging Systems and Tracker Agmentioning

confidence: 99%

Potential Therapeutic Mechanisms and Tracking of Transplanted Stem Cells: Implications for Stroke Treatment

Zhang

Yao

2017

Stem Cells International

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“…The greatest advantages of this image modality are the use of non-ionizing radiation, possible single-cell detection, and high spatial radiation (Cho et al 2012). Due to high sensitivity, non-radioactive CAs, and no cell damage, MRI assisted in drug delivery and cell tracking in the past several years (Fernandez-Fernandez et al 2011;Guo et al 2012a;Kraitchman and Bulte 2009;Ludtke-Buzug et al 2010).…”

Section: Mrimentioning

confidence: 99%

Lost signature: progress and failures in in vivo tracking of implanted stem cells

Haar

Lavrentieva

Stahl

et al. 2015

Appl Microbiol Biotechnol

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Stem cell therapy as a part of regenerative medicine provides promising approaches for the treatment of injuries and diseases. The increasing use of mesenchymal stem cells in various medical treatments created the demand for long-term in vivo cell tracking methods. Therefore, it is necessary to analyze post-transplantational survival, biodistribution, and engraftment of cells. Furthermore, stem cell treatment has been discussed controversially due to possible association with tumor formation in the recipient. For therapeutic purpose, stem cells must undergo substantial manipulation such as differentiation and in vitro expansion, and this can lead to the occurrence of genetic aberrations and altered expression of both tumor suppression and carcinogenic factors. To control therapy, it is necessary to find a reliable and general method to track and identify implanted cells in the recipient. This is especially challenging for autologous transplantations, as standard fingerprinting methods cannot be applied. An optimal technique for in vivo cell monitoring does not yet exist, and its development holds several challenges: small numbers of transplanted cells, possibility of cell number quantification, minimal transfer of the contrast agent to non-transplanted cells, and no genetic modification. This review discusses most of the proposed solutions, including magnetic resonance imaging, magnetic particle imaging, positron emission tomography, single-photon emission computed tomography, and optical imaging methods. Additionally, the recent research on cell labeling for stem cell monitoring after transplantation including in vitro, ex vivo, and in vivo imaging studies is described. Promising future imaging modalities for stem cell monitoring after transplantation are shown.

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“…Contrast agents result in the labeled cells presenting as hyperintense ( T 1 agents) or hypointense regions ( T 2 and T 2 * agents) on appropriately weighted MR images. Imaging using certain iron oxide agents can be very sensitive, even up to single cell imaging . Note that the use of MR contrast agents is also closely regulated, and they are not officially approved as cell labels.…”

Section: Introductionmentioning

confidence: 99%

Cell tracking using multimodal imaging

Srinivas

Melero

Kaempgen

et al. 2013

Contrast Media & Molecular

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In vivo imaging plays a key role in cell tracking, particularly for the optimization of cellular therapeutics. A recent trend is to use more than one imaging modality (multimodality imaging) for this purpose. There are several advantages to multimodal cell tracking, particularly the corroboration of data obtained using a new imaging agent or technique with an established one, and the ability to glean complementary information from a single experiment. In this review, we examine the different types of labels and imaging strategies used in the literature for multimodal cell tracking, and discuss the pros and cons of these approaches, with a focus on MRI. Despite many efforts and novel technologies, we still have to face situations where current imaging methods are simply not sensitive enough and new labeling strategies are hampered by the lack of approved reagents. Finally, we examine new in vitro and preclinical developments, which have the potential to tackle unresolved challenges in in vivo multimodal imaging.

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Detection of iron-labeled single cells by MR imaging based on intermolecular double quantum coherences at 14 T

Cited by 4 publications

References 42 publications

Potential Therapeutic Mechanisms and Tracking of Transplanted Stem Cells: Implications for Stroke Treatment

Potential Therapeutic Mechanisms and Tracking of Transplanted Stem Cells: Implications for Stroke Treatment

Lost signature: progress and failures in in vivo tracking of implanted stem cells

Cell tracking using multimodal imaging

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