Detection of Klebsiella pneumoniae cfDNA in pleural fluid and its clinical value

Ren, Min; Li, Li; Chu, Mingliang; Liu, Lin

doi:10.21037/apm-20-1574

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…Factors such as infection, intestinal microecological imbalance, and immune barrier disruption can cause conditional pathogenesis of Klebsiella pneumoniae . Previous studies have shown that Klebsiella pneumoniae -related sepsis can significantly increase the risk of systemic multi-site infections ( Li et al., 2021 ), and thoracoabdominal membranes are the potentially affected tissues ( Ren et al., 2020 ; Ameer et al., 2022 ). Interestingly, Klebsiella pneumoniae was only detected in multiple samples at the same time.…”

Section: Discussionmentioning

confidence: 99%

The value of next-generation metagenomic sequencing in pathogen detection of pleural effusions and ascites from children with sepsis

Liu

Wang²,

Li³

et al. 2023

Front. Cell. Infect. Microbiol.

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ObjectiveTo investigate the diagnostic value of metagenomic next-generation sequencing (mNGS) using pleural effusion and ascites from children with sepsis.MethodsIn this study, children with sepsis or severe sepsis and appeared pleural or peritoneal effusions were enrolled, of whom the pleural effusions or ascites and blood samples were conducted pathogen detection using both conventional and mNGS methods. The samples were divided into pathogen-consistent and pathogen-inconsistent groups based on the consistency of mNGS results from different sample types, and into exudate and transudate groups based on their pleural effusion and ascites properties. The pathogen positive rates, pathogen spectrum, consistency between different sample types, and clinical diagnosis consistency were compared between mNGS and conventional pathogen tests.ResultsA total of 42 pleural effusions or ascites and 50 other type samples were collected from 32 children. The pathogen positive rate of the mNGS test was significantly higher than that of traditional methods (78.57% vs. 14.29%, P < 0.001) in pleural effusion and ascites samples, with a consistent rate of 66.67% between the two methods. Nearly 78.79% (26/33) of mNGS positive results of the pleural effusions and ascites samples were consistent with clinical evaluation, and 81.82% (27/33) of these positive samples reported 1-3 pathogens. The pathogen-consistent group outperformed the pathogen-inconsistent group in terms of consistency with respect to clinical evaluation (88.46% vs. 57.14%, P = 0.093), while there was no significant difference between the exudate and transudate groups (66.67% vs. 50.00%, P = 0.483).ConclusionCompared to conventional methods, mNGS has great advantages in pathogen detection of pleural effusion and ascites samples. Moreover, consistent results of mNGS tests with different sample types provide more reference values in clinical diagnosis.

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Section: Discussionmentioning

confidence: 99%

The value of next-generation metagenomic sequencing in pathogen detection of pleural effusions and ascites from children with sepsis

Liu

Wang²,

Li³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…A research set up K . pneumoniae cell-free DNA (cfDNA) detection system, but the clinical application value still needs further study ( 8 ). Despite extensive research over the past few decades, the pathophysiology of K .…”

Section: Introductionmentioning

confidence: 99%

K. pneumoniae and M. smegmatis infect epithelial cells via different strategies

Hu,

Wan,

Liu

et al. 2023

J Thorac Dis

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Background As the first line of defense, epithelial cells play a vital role in the initiation and control of both innate and adaptive immunity, which participate in the development of disease. Despite its therapeutic significance, little is understood about the specific interaction between pathogenic microorganisms and lung epithelial cells. Methods In this study, we performed a head-to-head comparison of the virulence and infection mechanisms of Klebsiella pneumoniae ( K. pneumoniae ) and Mycobacterium smegmatis ( M. smegmatis ), which represent Gram-negative/positive respiratory pathogens, respectively, in lung epithelial cell models for the first time. Results Through scanning electron microscopy combined with bacterial infection experiments, we confirmed the ability of K. pneumoniae and M. smegmatis strains to form biofilm and cord factor out of the cell wall. M. smegmatis has stronger adhesion and intracellular retention ability, while K. pneumoniae is more likely to induce acute infection. These pathogens could stay and proliferate in lung epithelial cells and stimulate the secretion of specific cytokines and chemokines through a gene transcription regulator. M. smegmatis infection can promote crosstalk among epithelial cells and other immune cells in the lung from a very early stage by prompting the secretion of pro-inflammatory cytokines. Meanwhile, there were significant correlations between K. pneumonia infection and higher levels of interleukin-15 (IL-15), interleukin-1Rα (IL-1Rα), fibroblast growth factor (FGF) basic, and granulocyte colony-stimulating factor (G-CSF). At the same time, K. pneumonia infection also led to changes in the expression of cytoskeletal proteins in epithelial cells. Conclusions Our results emphasized the immunoprotection and immunomodulation of lung epithelial cells against exogenous pathogenic microorganisms, indicating that different pathogens damaged the host through different strategies and induced varying innate immune responses. At the same time, they provided important clues and key immune factors for dealing with complicated pulmonary infections.

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Diagnostic value of soluble biomarkers for parapneumonic pleural effusion

Cao

Zheng

2023

Critical Reviews in Clinical Laboratory Sciences

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Detection of Klebsiella pneumoniae cfDNA in pleural fluid and its clinical value

Cited by 3 publications

References 28 publications

The value of next-generation metagenomic sequencing in pathogen detection of pleural effusions and ascites from children with sepsis

The value of next-generation metagenomic sequencing in pathogen detection of pleural effusions and ascites from children with sepsis

K. pneumoniae and M. smegmatis infect epithelial cells via different strategies

Diagnostic value of soluble biomarkers for parapneumonic pleural effusion

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