Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea

Gomez, Dennis K.; Joh, Seong Joon; Jang, Hwan; Shin, Sook; Choresca, Casiano H.; Han, Jee Eun; Kim, Ji Hyung; Jun, Jin Woo; Park, Taesung

doi:10.1016/j.aquaculture.2010.11.021

Cited by 25 publications

(29 citation statements)

References 26 publications

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“…Similarly, CyHV-3 spread rapidly in Indonesia with disease outbreaks reported on most of the major islands by 2006 (Lio-Po, 2011). CyHV-3 has also been detected in China (Dong et al, 2011), South Korea (Gomez et al, 2011), Singapore (Lio-Po, 2011), Malaysia (Musa, Leong, & Sunarto, 2005), and Thailand (Lio-Po, 2011;Pikulkaew, Meeyam, & Banlunara, 2009).…”

Section: Geographical Distribution and Prevalencementioning

confidence: 93%

Cyprinid Herpesvirus 3

Boutier

Ronsmans

Rakus

et al. 2015

Advances in Virus Research

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Section: Geographical Distribution and Prevalencementioning

confidence: 93%

Cyprinid Herpesvirus 3

Boutier

Ronsmans

Rakus

et al. 2015

Advances in Virus Research

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“…Using PCR tests (OIE 2013), KHV was detected in tissue samples from apparently healthy seed stock of cultured common carp and Siberian crucian carp, with a detection rate of 15.5% in 2010, similar to the detection rate of 15% (6/41 fish) in common carp and Israeli carp Cyprinus carpio nudus reported by Kim et al (2010) for fish farms that had previously undergone mass mortalities between 1999 and 2007. In addition, KHV was detected in heavily infected brood stock of koi carp farms in Korea (Gomez et al 2011). However, ours is the first report of KHV detected in apparently healthy carp in Korea and in any Siberian crucian carp in Korea.…”

Section: Discussionmentioning

confidence: 59%

Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

Cho¹,

Won²,

Kim³

et al. 2014

Dis. Aquat. Org.

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Koi herpesvirus (KHV) disease is a lethal disease in common carp, an important food fish in Asian countries, the seed of which is used in restocking programs for freshwater fishery management. We inspected apparently healthy seed stock of common carp Cyprinus carpio L. and Siberian crucian carp Carassius auratus for the presence of KHV using PCR-based diagnostic tests as a part of a stock enhancement program from 2009 to 2010 in Korea. Consequently, KHV was detected from 24 of 232 inspections with yearly detection percentages of 5.2% in 2009 and 15.5% in 2010 using PCR primer sets for TK or SphI-5 as recommended by the OIE Manual of Diagnostic Tests for Aquatic Animals. Results indicate that the SphI-5 primer set was slightly more sensitive than the TK primer set, as shown by a higher detection rate. To determine the genotype of the KHV strains detected in this study, ORF40-specific PCR amplification was conducted, and the PCR products from 6 samples showed 100% nucleotide sequence identity with a Japanese strain (GenBank accession number AP008984) but not with US (DG657948) and Israeli strains (DG177346). This report conclusively demonstrated the presence of KHV in externally healthy seed of common carp and Siberian crucian carp, indicating a possible risk that subclinically infected seed stock can be released with a potential threat to wild populations. KEY WORDS: Carp · Cyprinus carpio · Carassius auratus · Cyprinid herpesvirus 3 · PCR · ORF40Resale or republication not permitted without written consent of the publisher Dis Aquat Org 112: [29][30][31][32][33][34][35][36] 2014 Marine and inland stock enhancement by releasing aquatic animals raised in hatcheries has been considered a potential management tool for increasing production and profitability of fisheries and meeting the demand for food requirements for an ever-increasing human population (Bartley et al. 2006). In Korea, hatchery-reared carp seed, especially common carp and Siberian crucian carp, have been released into the environment for the purpose of restoring the indigenous fish species and replenishing severely depleted fisheries in inland ranching programs.Concerns about unfavorable consequences of hatchery-reared seed releases such as the spread of pathogens, competition for food, and cannibalism, as well as genetic deterioration of wild stocks, have been increasing within the framework of aquatic animal health management (Fushimi 2001). Subasinghe (2005) also pointed out that movements of live aquatic animals and animal products (broodstock, seed, and feed) have accelerated the accidental spread and incursion of diseases into new populations and geographic regions, for example, through movements of hatchery-produced stocks. Thus special efforts have been required to prevent disease spread due to the release of cultured seed into the ecosystem, in terms of aquatic animal health management (Blankenship & Leber 1995).To prevent transmission of infectious agents by releasing hatchery-raised seed into the wider environment as part of...

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“…A positive reaction is shown by the absence of a dot at the bottom of a well. So the magnitude of the value of adhesion molecules reaches 2 6 . The induction of crude protein in the organs Halimeda sp both eyes and brain test showed a positive reaction to the dilution 1/32.…”

Section: Resultsmentioning

confidence: 99%

“…The brood stocks showed poor appetite, lethargy, abnormal swimming behaviour with gasping movements in shallow water and increased mucus secretion. Histopathologically, the gill showed diffuse necrosis of the bronchial epithelial cells and diffuse lymphocyticmonocytic interstitial nephritis with necrosis of the tubular epithelial cells [6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Crude Protein Halimeda Sp. On Cyprinus Carpio Infected Koi Herpes Virus on Expression of Major Histocompatibility Complex Class-1

Yanuhar

Wahyuningtyas

2017

JFMR

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Halimeda sp. is a species of macroalgae that grow abundant in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the treatment of crude protein Halimeda sp on Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like in this C. carpio. The methods are isolation of crude protein Halimeda sp, purification of crude protein, Haemagglutination (HA) test and dot blot test. The result shows crude protein of Halimeda sp. can produce adaptive immune response like MHC class 1. MHC class-1 has the function of the immune system for maintenance of direct virus invasion. Responsible to treatment of crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The conclusion is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..

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Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea

Cited by 25 publications

References 26 publications

Cyprinid Herpesvirus 3

Cyprinid Herpesvirus 3

Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

The Effect of Crude Protein Halimeda Sp. On Cyprinus Carpio Infected Koi Herpes Virus on Expression of Major Histocompatibility Complex Class-1

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