Detection of lead in blood, seminal plasma, and spermatozoa of bulls. Effectin vitro of lead acetate on sperm motility

Alexaki, E.; Samara, Constantini; Alexopoulos, C.; Tsafaris, F; Smokovitis, A.

doi:10.1007/bf01701078

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…Lead exposure has been reported to reduce steroid metabolic activity in SCs and to be cytotoxic for Sertoli-germ cell cocultures (Adhikari et al, 2000;Wiebe et al, 1983). Furthermore, exposure of animals (Alexaki et al, 1990) and humans (Huang et al, 2001) to lead results in decreased sperm motility. In vivo exposure of mice to lead induces mitochondrial changes in SCs (Bizarro et al, 2003) and loss of normal testicular structure (Shafai et al, 2011).…”

Section: Evidence From Heavy Metals Toxicological Studiesmentioning

confidence: 99%

“…Heavy metals Cadmium Clough et al, 1990Janecki et al, 1992Clough et al, 1990Hew et al, 1993Wong et al, 2004El-Demerdash et al, 2004Foote 1999;Pant et al, 2003;Telisman et al, 2000;Tvrdá et al, 2013Mercury Monsees et al, 2000-Monsees et al, 2000Keck et al, 1993Keck et al, 1993Lead Batarseh et al, 1986Adhikari et al, 2000Batarseh et al, 1986Bizarro et al, 2003Alexaki et al, 1990Hsu et al, 1997;Huang et al, 2001;Kasperczyk et al, 2008;Shafai et al, 2011 Chemicals continuously exposed and/or subjected to high concentrations, which may cause health problems, including sub...…”

Section: Sperm Parametersmentioning

confidence: 99%

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Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Reis

Moreira

Sousa

et al. 2015

J of Applied Toxicology

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Pressure towards population aging in the demographic pyramid is not only due to sociological/personal choices but also due to subfertility or infertility. There are several chemicals and mixtures that impair male fertility. While experimental animal models are crucial to identify compounds that affect male fertility, it is essential to use reliable in vitro models to determine cellular targets and intracellular pathways that mediate chemical toxicity in the male reproductive system. In this review, we focused on the somatic Sertoli cell (SC) that, within the testis, is a major target for hormonal signaling and provides physical and nutritional support to developing germ cells. The different outcomes possible in each type of study: in vivo versus in vitro (either in primary or immortalized cell cultures) are analyzed. Herein, we intend to clarify the unique features that render SCs as excellent candidates for a robust in vitro model to study the deleterious effects of chemicals on male reproductive health. The sensitivity of SCs to toxicants/pharmaceuticals is discussed and, based on the literature reviewed we propose the in vitro study of SC physiology as a model to disclose deleterious effects of substances to male fertility.

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Section: Evidence From Heavy Metals Toxicological Studiesmentioning

confidence: 99%

Section: Sperm Parametersmentioning

confidence: 99%

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Reis

Moreira

Sousa

et al. 2015

J of Applied Toxicology

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“…Two studies have shown higher lead concentrations in semen from infertile men attending fertility clinics, as compared to fertile control subjects [Saaranen et al, 1987;Jockenhövel et al, 1990], but the reason for such difference is unclear. The concentrations found in seminal plasma may be compared to the high level of 0.25 µg/ml (1.21 µmol/l) which resulted in only slight decreases (not statistically significant) in bull sperm motility in vitro [Alexaki et al, 1990]. Thus, although occupational lead exposure seems to cause both an increased lead concentration in seminal plasma and a decrease in sperm quality, an overall association between the two parameters does not necessarily reflect a direct effect on sperm caused by lead present in seminal plasma.…”

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confidence: 96%

Improvement in semen quality associated with decreasing occupational lead exposure

Viskum

Rabjerg

Jørgensen

et al. 1999

American J Industrial Med

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Background Cross‐sectional studies have suggested that occupational lead exposure may adversely affect sperm quality. >Methods Sperm quality changes were prospectively assessed in 19 men employed at a car battery plant where efforts were made to decrease the exposure level. The participants delivered monthly samples of semen and venous blood during their employment at the factory. The factory then closed, and additional samples were obtained from 16 of the men. Results Average blood‐lead concentrations decreased from 2.03 >mol/l to 0.96 >mol/l during the observation period. Concomitantly, significant improvements were seen in the proportion of motile cells both at sample delivery and after 24 hr, and in penetration. However, the sperm cell concentration and the proportion of morphological abnormalities did not change. Conclusions These results support the notion that occupational lead exposure at currently acceptable levels has a small adverse effect on sperm quality, especially sperm motility, and that this effect is at least partially reversible. Am. J. Ind. Med. 35:257–263, 1999. © 1999 Wiley‐Liss, Inc.

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“…Hg2+, Cu2+, and Fez+, but not by Mn2+ and Co2+ (White, 1955;Alabi et al, 1985;Kanwar et al, 1988;Alexaki et al, 1990;Ernst and Lauritsen, 1991;Riff0 et al, 1992). Sperm are most sensitive to mercury.…”

Section: Introductionmentioning

confidence: 92%

“…Motility of sperm from various species is inhibited by Pb2+, Zn2+, Cd2+, 0 1995 WILEY-LISS, INC. *This article is a US Government work and, as such, is in the public domain in the United States o f America. Hg2+, Cu2+, and Fez+, but not by Mn2+ and Co2+ (White, 1955;Alabi et al, 1985;Kanwar et al, 1988;Alexaki et al, 1990;Ernst and Lauritsen, 1991;Riff0 et al, 1992). Sperm are most sensitive to mercury.…”

Section: Introductionmentioning

confidence: 99%

Action of metallic ions on the precocious development by rabbit sperm of motion patterns that are characteristic of hyperactivated motility

Young¹,

Bodt

Heitkamp³

1995

Molecular Reproduction Devel

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An earlier study demonstrated that rabbit sperm incubated for 16 hr under capacitation conditions acquire motility patterns identical to those seen in rabbit sperm capacitated in vivo. We now show that similar motion patterns develop after 0.5 hr incubation in a Tris-buffered medium, medium M. Development and decline of the motion patterns occurred in three phases each recognized by the character of the biphasic motion patterns. Hyperactivated sperm were objectively identified and quantified by a previously developed computer-directed model. The percentage of motile sperm that acquired hyperactivated motility and the period they remained in this state varied among sperm from different rabbits. The decline in hyperactivated motility was paralleled by a decrease in the average sperm curvilinear velocity (VCL) and average amplitude of lateral head displacement (AALH), but was not accompanied by a concomitant decrease in percentage of motile sperm. Pb2+ and Cd2+, at concentrations that did not inhibit motility, prevented development of hyperactivated motility. Inhibition of hyperactivated motility by Pb2+ was time- and concentration-dependent; the average percentage of hyperactivated sperm decreased from approximately 30% to < 5% (n = 5) in 1 hr at a Pb2+ concentration of 25 microM. Cd2+ inhibition of hyperactivation was dependent only on concentration of the cation. At a concentration of 100 microM, the decrease in the percent of hyperactivated sperm was approximately 50% (n = 3). Hg2+, Zn2+, and Cr6+ at sublethal concentrations had no effect on hyperactivated motility development. These results suggest that Pb2+ and Cd2+, by virtue of their ability to prevent the wide curvature flagella beating that is characteristic of hyperactivation, can compromise fertilization at concentrations that do not inhibit sperm motility and act as a reproductive toxicant at a level other than spermatogenesis.

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Detection of lead in blood, seminal plasma, and spermatozoa of bulls. Effectin vitro of lead acetate on sperm motility

Cited by 11 publications

References 17 publications

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Improvement in semen quality associated with decreasing occupational lead exposure

Action of metallic ions on the precocious development by rabbit sperm of motion patterns that are characteristic of hyperactivated motility

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