Detection of Live Salmonella sp. Cells in Produce by a TaqMan-Based Quantitative Reverse Transcriptase Real-Time PCR Targeting invA mRNA

Abstract

Salmonella enterica contamination in foods is

Influence of Temperature and Predation on Survival of Salmonella enterica Serovar Typhimurium and Expression of invA in Soil and Manure-Amended Soil

“…This gene is highly conserved in almost all Salmonella serotypes (7,17,44), and detection of Salmonella based on the presence of this gene has previously been reported (11,12,24). invA mRNA has been used as a biomarker for active cells (13,18,26); it has been discussed, however, whether transcription of the invA gene may differ with different physiological states of the cell, which may affect assay specificity (13). Jacobsen and Holben (26) showed a detection limit of 5 ϫ 10 4 seeded Salmonella serovar Typhimurium cells per g of soil.…”

“…The introduction of molecular techniques has become an especially important advance in reducing the time required for detection of Salmonella and in detecting active bacteria in environmental samples through their DNA and RNA (10,18,51).…”

Multiplex TaqMan Real-Time PCR (qPCR) Assay Targeting prot6E and invA Genes for Fast and Accurate Detection of Salmonella Enteritidis

“…The inclusion of this internal control did not affect either the amplification or the detection limit of the qPCR assay. The qPCR developed here as opposed to an invA single target qPCR method (Feder et al, 2001;Malorny et al, 2004;Bohaychuk et al, 2007;Gonzalez-Escalona et al, 2009) is able to detect specifically SE strains by the use of an SE specific marker (prot6E). Additionally it is capable to detect other SE that might lack the prot6E gene (Malorny et al, 2007a), such as the case for SE-10.…”

Nucleic Acid-Based Methods to Identify, Detect and Type Pathogenic Bacteria Occurring in Milk and Dairy Products

“…To address the need of detecting only living pathogens RNA may be detected rather than DNA [151][152][153][154][155].…”