Detection of liver cancer cells by using ELISA and coupling of anti-glypican 3 antibody and magnetite nanoparticles

Trung, Thanh Bui; Hung, Van Pham; Hoang, Hai Tran; Tung, Le Minh; Lee, Jaeryeong

doi:10.1080/12269328.2015.1070698

Cited by 8 publications

(1 citation statement)

References 31 publications

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“…MNPs bound with ScFv (fragment antibody) and anti‐CD73 antibody magnetically separated 5′‐nucleotidase enzyme used as a bioindicator for diagnosing diseases . Anti–glypican 3 antibody‐conjugated MNPs can be used to detect glypican 3 liver cancer cells with enzyme‐linked immunosorbent assay . In addition, MNPs coated with poly‐(ethylene oxide)‐ block ‐poly(2‐vinyl‐4,4‐dimethylazlactone) diblock copolymers were used as a recyclable magnetic nanosupport for adsorption with anti–rabbit immunoglobulin G antibody …”

Section: Introductionmentioning

confidence: 99%

Reusable magnetic nanocluster coated with poly(acrylic acid) and its adsorption with an antibody and an antigen

Meerod

Deepuppha

Rutnakornpituk

et al. 2017

J of Applied Polymer Sci

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The synthesis of negatively charged magnetite nanoclusters grafted with poly(acrylic acid) (PAA) and their application as reusable nanosupports for adsorption with antibodies and antigens are presented in this article. They were facilely prepared via the free-radical polymerization of PAA in the presence of functionalized magnetite nanoparticles to obtain highly negative charged nanoclusters with a high magnetic responsiveness and good dispersibility and stability in water. According to transmission electron microscopy, the sizes of the nanoclusters ranged between 200 and 500 nm, without large aggregation visually observed in water. The hydrodynamic size of the nanocluster consistently increased with increasing pH of the dispersion; this indicated its pH-responsive properties, which was due to the repulsion of the anionic carboxylate groups in the structure. This nanocluster was successfully used as an efficient and reusable support for adsorption with anti-horseradish peroxidase antibody. It preserved higher than a 97% adsorption ability of the antibody after eight reuse cycles; this signified the potential of this novel nanocluster as a reusable support in the magnetic separation applications of other bioentities.

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Section: Introductionmentioning

confidence: 99%