Detection of lymphocytes productively infected with Epstein–Barr virus in non-neoplastic tonsils

Ikeda, Tetsuya; Kobayashi, Ryo; Horiuchi, Manabu; Nagata, Y.; Hasegawa, Makoto; Mizuno, Fumio; Hirai, Kanji

doi:10.1099/0022-1317-81-5-1211

Cited by 31 publications

(31 citation statements)

References 35 publications

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“…14 Additionally, viral proteins found in latent and replicative infection may be identified by immunohistochemical techniques. [2][3][4]13 The tonsils are considered the site of initial infection, and of viral persistence and replication. The EBV may infect the tonsils of children and become involved in recurring tonsillitis.…”

Section: Discussionmentioning

confidence: 99%

“…Many researchers have suggested that the tonsils are a possible replication site for this virus. 2,3,8,13 Molecular techniques have been used often for diagnosing and monitoring patients with virus diseases. The EBV-DNA can be identified by in situ hybridization (ISH) and the polymerase chain reaction (PCR); some authors consider both equally sensitive for detecting the EBV.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have suggested that virus infections may be involved in recurring infections, including the Epstein-Barr virus (EBV) and herpes simplex viruses. [2][3][4] The EBV was discovered in 1964 in a culture of Burkitt's lymphoma cells; in 1968 studies revealed that it caused infectious mononucleosis. 5 It is a herpesviridae of the subfamily gamaherpesvirinae, which infects most individuals before adult life.…”

Section: Introductionmentioning

confidence: 99%

“…Infectious mononucleosis and oral hairy leukoplakia have been used as models for studying the replication mechanism of the virus. 3 The EBV replication mode in healthy subjects is still unknown. The tonsils appear to be candidate sites for EBV replication.7,8 Babcock (1998) detected linear episomal forms of EBV DNA in tonsillary lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

“…Until recently it was thought that the virus was capable of infecting only B lymphocytes and epithelial cells; there have, however, been reports of infected normal T lymphocytes. 3 The EBV is associated not only with infectious mononucleosis but also with other benign diseases, such as oral hairy leukoplakia, and malignancies, such as Hodgkin's lymphoma, non-Hodgkin's B and T cell lymphomas, and nasopharyngeal, gastric and breast carcinomas. It is currently being associated with autoimmune diseases, such as lupus erythematosus and multiple sclerosis.…”

Section: Introductionmentioning

confidence: 99%

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Detecção do vírus Epstein-Barr em tonsilites recorrentes

Dias

Rocha

Carvalho³

et al. 2009

Rev. Bras. Otorrinolaringol.

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Recurrent tonsillitis has been the subject of frequent investigation. Misuse of antibiotic therapy in acute tonsillitis, changes to the tonsillar microflora, structural changes to the tonsillar crypts, and viral infections have been listed as predisposing or causal factors for recurrent tonsillitis. Epstein-Barr virus (EBV) infection usually occurs in early childhood and may persist in tonsillar lymphocytes, thus leading to the onset of recurrent tonsillitis. Little is known about the persistence and reactivation of EBV strains in immunocompetent patients. Methods such as in situ hybridization, polymerase chain reaction (PCR), and immunochemistry have been used to study the pathogenesis of the EBV. Aim: this study aims to characterize the association between EBV and recurrent tonsillitis by investigating the presence of EBV through PCR and immunohistochemistry, using viral protein LMP-1 as a target. Study design: this is a cross-sectional study with analysis of sample prevalence. Materials and method: twenty-four paraffin-embedded tonsil specimens from the Pathology Service were selected. The specimens were removed from children aged between 2 and 12 years diagnosed with recurrent tonsillitis. Results: EBV genome was detected in 13 (54.1%) specimens, whereas viral protein LMP-1 was found in 9 (37.5%) specimens. Conclusion: children's tonsils can be colonized by EBV and such colonies may be associated with the pathogenesis of recurrent tonsillitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detecção do vírus Epstein-Barr em tonsilites recorrentes

Dias

Rocha

Carvalho³

et al. 2009

Rev. Bras. Otorrinolaringol.

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Distribution patterns of β‐ and γ‐herpesviruses within Waldeyer's ring organs

Berger

Hug

Gysin

et al. 2007

Journal of Medical Virology

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The Waldeyer's ring designates a functional unit of lymphoid tissue within the pharynx including the adenoids and tonsils. To gain insight into distribution patterns of beta- and gamma-human herpesviruses (HHVs) and their potential mutual influences at their natural portal of entry, quantitative polymerase chain reaction (qPCR) assays were applied to adenoids and tonsils obtained from 30 children. DNA of Epstein-Barr virus (EBV), cytomegalovirus (CMV), HHV-6, HHV-7, and HHV-8 was detected in adenoids, tonsils, or both of 24 (80%), 19 (63%), 23 (77%), 23 (77%), and 0 (0%) children, respectively. EBV, CMV, HHV-6, and -7 localized in both adenoids and tonsils from 92%, 37%, 52%, and 70% of children, respectively, with the virus detectable by qPCR. The amount of EBV was 2-10-fold higher than of other HHVs and correlated in autologous organs (P = 0.01) as did the amount of HHV-7 (P = 0.002). The amount of CMV correlated with the HHV-6 amount in adenoids (P = 0.028) and tonsils (P = 0.007), and with the amount of HHV-7 in adenoids (P < 0.01). Levels of HHV-6 DNA were lower in adenoids with detectable CMV DNA than in adenoids without detectable CMV DNA (P = 0.0062). Inversely, CMV and HHV-7 levels were higher in adenoids with than in adenoids without detectable EBV DNA (P = 0.019 and P = 0.039, respectively).Thus, beta- and gamma-HHV exhibit distinct distribution behaviors in Waldeyer's ring organs and seem to interact. This may be of medical importance in immunocompromised hosts who are likely to reactivate HHVs causing severe morbidity and death.

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Epstein‐Barr virus (EBV) DNA levels in palatine tonsils and autologous serum from EBV carriers

Nadal

Blasius

Niggli

et al. 2002

Journal of Medical Virology

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A real-time polymerase chain reaction was employed to detect and quantitate Epstein-Barr virus (EBV) DNA in tonsils and autologous sera from EBV-seropositive children. EBV DNA was found in 95% of tonsils from 21 children and in 50% from 18 children with serum IgG titers to the virus capsid antigen (VCA) of > or =1:160 and 1:10 to 1:80, respectively (P = 0.002). Tonsils from children with titers > or =1:160 harbored more EBV DNA copies per mg tissue (mean, 1,237; range, < 2-13,998) than from children with titers 1:10 to 1:80 (mean, 23; range, < 2-226; P < 0.0001). By contrast, EBV DNA was detected only in serum from 25% of 20 children with titers > or = 1:160. Thus, ample differences in tonsillar EBV replication are mirrored inconstantly by detectable EBV in autologous serum suggesting that EBV DNA quantitation in tonsils may serve for refined monitoring of individuals at risk of EBV-associated lymphoproliferation.

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Detection of lymphocytes productively infected with Epstein–Barr virus in non-neoplastic tonsils

Cited by 31 publications

References 35 publications

Detecção do vírus Epstein-Barr em tonsilites recorrentes

Detecção do vírus Epstein-Barr em tonsilites recorrentes

Distribution patterns of β‐ and γ‐herpesviruses within Waldeyer's ring organs

Epstein‐Barr virus (EBV) DNA levels in palatine tonsils and autologous serum from EBV carriers

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