Detection of main urinary metabolites of β2-agonists clenbuterol, salbutamol and terbutaline by liquid chromatography high resolution mass spectrometry

Identification of the major metabolites of (R)‐salbutamol in human urine, plasma and feces using ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

“…The MS2 spectrum of (R)‐salbutamol with the proposed mechanism of the composition is shown in Figure . The [M+H] + ion of (R)‐salbutamol was identified at m/z 240.1597, with three typical fragment ions at m/z 222.1493, 166.0871, and 148.0764, which was consistent with the previous report . The fragment ion at m/z 222.1493 was formed via the loss of a molecule of H 2 O.…”

“…The fragment ion at m/z 130.0651 was formed by the loss of four oxygen atoms, four methylene groups, and two molecules of H 2 . M11 was also in accordance with the previous study .…”

“…By further losing a methylene group from fragment m/z 119.0855, the fragment ion at m/z 105.0695 was formed. M12 was also in accordance with the previous study .…”

Determination of clenbuterol in pork and potable water samples by molecularly imprinted polymer through the use of covalent imprinting method

“…Many analytical methods including enzyme-linked immunosorbent assay (ELISA) (Prezelj, Obreza, & Pecar, 2003), high performance liquid chromatography (HPLC) (Botterblom, Feenstra, & Erdtsieck-Ernste, 1993;Chang, Chou, & Hwang, 2005;Morales-Trejo, León, Escobar-Medina, & Gutiérrez-Tolentino, 2013), gas chromatography coupled with mass spectrometry (GC-MS) (Amendola, Colamonici, Rossi, & Botrè, 2002;Ramos et al, 2003), and liquid chromatography coupled with mass spectrometry (LC-MS) (Blanca et al, 2005;Courant et al, 2009;Dominguez-Romero et al, 2013), for screening CLB residue had been reported. A sample pretreatment and concentration step was required during the analytical process, especially to detect CLB in complex samples at ultratrace concentrations.…”

Evaluation of the use of solvent impregnated resins in the analysis of salbutamol in human urine followed by capillary electrophoresis

“…In most cases, the quantification of SB in urine requires the pretreatment of the sample by liquid-liquid extraction (LLE) [12] or solid phase extraction (SPE) with different types of no-polar cartridges (silica gel with octadecyl or carboxylic acid, SCX Extract-Clean, ASPEC, DECs, and polymeric Bond Elut PLEXATM SPE cartridge) [13][14][15][16][17]. Although these techniques are very popular for the separation and concentration of inorganic and organic substances, they have some disadvantages like the large consumption of solvents and the occasional clogging of the cartridges during the handling of real samples [18].…”