Detection of MBL Production in Pseudomonas Aeruginosa in Katihar District, Bihar

Abstract

BACKGROUNDPseudomonas aeruginosa is a leading cause of nosocomial infection due to its numerous intrinsic and acquired mechanisms of drug resistance. Although antibiotic resistance in P. aeruginosa is caused by multiple mechanisms, one major factor leading to resistance is the production of carbapenemases. Metallo-β-lactamases (MBLs) are carbapenemases which require zinc at the active site and are predominantly produced by P. aeruginosa. The selection criteria for confirmation of MBL producers are reduced susceptibility or resistance to carbapenems and/or ceftazidime.The aim of the study was to determine the efficacy of three antibiotics viz., meropenem, imipenem and ceftazidime as a screening and confirmatory agent for detection of MBL and to assess the diagnostic potential of DDST, DPT and MIC reduction test for detection of MBL.