Detection of Methicillin-Resistant Staphylococcus aureus in Clinical Specimens from Cystic Fibrosis Patients by Use of Chromogenic Selective Agar

Perez, Leandro Reus Rodrigues; Antunes, Ana Lúcia Souza; Bonfanti, Jéssica Weiss; Pinto, Jaqueline Becker; Roesch, Eliane Würdig; Rodrigues, Diógenes; Dias, Cícero

doi:10.1128/jcm.00549-12

Cited by 8 publications

(2 citation statements)

References 19 publications

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“…Patients with MRSA infection were described to have frequent exacerbations and poorer lung function; thus, infection control is important, and patients should be adequately monitored [ 10 ]. A chromogenic selective medium for MRSA detection may improve its surveillance in patients with CF [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The first report of Methicillin-resistant Staphylococcus aureus (MRSA) in cystic fibrosis (CF) patients in Saudi Arabia

Banjar

Al-Qahtani

et al. 2020

International Journal of Pediatrics and Adolescent Medicine

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Introduction Methicillin-resistant Staphylococcus aureus infections have been increasingly reported in patients with cystic fibrosis (CF) who have progressive deterioration in their pulmonary function. Objectives To determine the prevalence of MRSA infections in CF in a tertiary care center in Saudi Arabia. Methodology This is a retrospective chart review conducted as part of the CF registry data from 1 January 2002 to 1 June 2016. All patients with confirmed CF of all age groups who had a respiratory culture positive for MRSA were included in the study. Results Among 385 patients with CF who had respiratory samples, 43 (11%) were positive for MRSA at a mean age of 10.4 ± 7.2 years. Twenty-two patients out of the 43 (51%) were treated with different regimens: nasal Bactroban in 13/22 (59%); a combination of nasal Bactroban, oral vancomycin, and rifampicin for 2 weeks in 5 patients (23%); Bactroban and linezolid in one patient (5%); and oral vancomycin and rifampicin in 3 patients (14%). Eight out of the 22 treated patients (36%) achieved MRSA eradication. Six out of the 22 treated (27%) had experienced MRSA recurrence within 3–6 months, and another 5/22 (23%) continued to have MRSA colonization up to 2–4 years of follow-up despite using a proper eradication protocol. Twelve out of the 43 (28%) patients with MRSA infection died. Conclusion MRSA infection in our population with CF is common. Therefore, an eradication protocol should be instituted at an early stage to prevent chronic colonization. Children with persistent MRSA colonization have high morbidity and mortality rate.

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Section: Introductionmentioning

confidence: 99%

The first report of Methicillin-resistant Staphylococcus aureus (MRSA) in cystic fibrosis (CF) patients in Saudi Arabia

Banjar

Al-Qahtani

et al. 2020

International Journal of Pediatrics and Adolescent Medicine

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“…Genotypic identification can be done with the phenotypic approach together for a better comparative analysis. Furthermore, the introduction of molecular screening for MRSA detection as well as identification directly from clinical specimens has been developed to enhance and identify common Staphylococcal spp., as well as to speed up the detection methods especially in clinical research [4].…”

Section: Introductionmentioning

confidence: 99%

Genotyping Approaches for Identification and Characterization of Staphylococcus aureus

Al-Obaidi¹,

Suhaili²,

MohdDesa³

2018

Genotyping

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Genotyping methods are vital epidemiological tools for discriminating different bacterial isolates within same species, which in turn provide useful data in tracing source of infection and disease management. There have been a revolutionary efforts in ways to distinguish between bacterial types and subtypes at molecular level utilizing DNA in the genomes. Notably, the growth of various DNA typing methods has provided innovative apparatuses for improved surveillance and outbreak investigation. Thus, early identification and genotyping are indispensable as resources for managing therapeutic treatment and the control of rapid expansion of clinically important bacteria. Methicillin-resistant Staphylococcus aureus (MRSA) has been in a great attention due to its contagious nature and subjected to various typing analyses. Thus, in this chapter, we aimed to review the contribution of various genotyping methods of commonly used as well as those unique to staphylococci in understanding its epidemiology, infection and dissemination pattern, and to provide an impression of specific advantages and disadvantages of each tool.

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Special Phenotypic Methods for Detecting Antibacterial Resistance

Simner,

Humphries

2023

ClinMicroNow

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Special phenotypic tests may be performed as an adjunct or surrogate to standard antimicrobial susceptibility testing methods to help characterize an isolate's antimicrobial susceptibility to a drug or drug class or detect a particular resistance mechanism or phenotype. Special phenotypic susceptibility tests are categorized into supplemental tests, screening tests, surrogate agent tests, and equivalent agent tests. Phenotypic methods for the detection of resistance have been developed for use with both clinical specimens and cultured growth. Resistance to streptomycin is mediated by a different resistance mechanism, and consequently, streptomycin resistance must be determined separately from gentamicin resistance. Although erythromycin and clindamycin are in separate antimicrobial agent classes, i.e., macrolides and lincosamides, respectively, their mechanisms of action and mechanisms of resistance are similar. Antimicrobial resistance among Gram‐negative bacilli, and especially resistance to β‐lactam agents, is a serious public health concern worldwide.

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Detection of Methicillin-Resistant Staphylococcus aureus in Clinical Specimens from Cystic Fibrosis Patients by Use of Chromogenic Selective Agar

Cited by 8 publications

References 19 publications

The first report of Methicillin-resistant Staphylococcus aureus (MRSA) in cystic fibrosis (CF) patients in Saudi Arabia

The first report of Methicillin-resistant Staphylococcus aureus (MRSA) in cystic fibrosis (CF) patients in Saudi Arabia

Genotyping Approaches for Identification and Characterization of Staphylococcus aureus

Special Phenotypic Methods for Detecting Antibacterial Resistance

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