Detection of microRNA in clinical tumor samples by isothermal enzyme-free amplification and label-free graphene oxide-based SYBR Green I fluorescence platform

Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection

“…It is still ambiguous whether dsDNA containing several singlestranded toeholds cannot be effectively adsorbed by GO [45,46], or whether it can be anchored onto the GO surface by its single stranded toeholds (as shown in Fig. 3A) [47,48].…”

A Graphene-enhanced imaging of microRNA with enzyme-free signal amplification of catalyzed hairpin assembly in living cells

“…Owing to recent advances in molecular engineering and programming of DNA nanotechnology, nucleic acid hybridization-based nanocircuits have allowed the detection of nucleic acid targets with tens-or even hundredsfold catalytic amplification (Cheglakov et al, 2015;Wu et al, 2015). Recently, the catalyzed hairpin assembly (CHA) has been shown to be an idea choice for amplification and has been widely used for detection of DNA, proteins and miRNA in buffer solution (Huang et al, 2012;Liu et al, 2015;Zhu et al, 2014).…”

Target-catalyzed hairpin assembly and intramolecular/intermolecular co-reaction for signal amplified electrochemiluminescent detection of microRNA

“…where I was the ECL intensity and c was the concentration of miR-21. In addition, we made a comparative work of the proposed biosensors in miR-21 detection with previous reported Zhu et al, 2015;Yin et al, 2012;Zhang et al, 2015;Liu et al, 2014a;Hao et al, 2014;Liu et al, 2014b) (shown in Table S2). …”