Detection of multiple viruses in oropharyngeal samples from Brazilian free-tailed bats (Tadarida brasiliensis) using viral metagenomics

Cibulski, Samuel Paulo; Lima, Francisco Esmaile de Sales; Teixeira, Thaís Fumaco; Varela, Ana Paula Muterle; Scheffer, Camila Mengue; Mayer, Fabiana Quoos; Witt, André Alberto; Roehe, Paulo Michel

doi:10.1007/s00705-020-04825-x

Cited by 20 publications

(21 citation statements)

References 30 publications

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“…The 18 novel genomoviruses identified in this study were phylogenetically placed into four different viral genera and founded 17 novel viral species. Since the identification of the first genomovirus identified by our group in a New World bat species -specifically, in oral/anal swabs of T. brasiliensis [11]-only one other genomovirus has been identified in any species of New World bats [65]. The novel smacovirus identified in this study is the first smacovirus found in association with bats, and it was classified as a novel species of the genus Porprismacovirus.…”

Section: Discussionmentioning

confidence: 65%

Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

et al. 2022

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Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.

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Section: Discussionmentioning

confidence: 65%

Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

et al. 2022

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“…Tadarida brasiliensis is one of the most widespread mammalian species in the Western Hemisphere, inhabiting a wide range of urban and wild environments. Researchers have isolated and identified a variety of viruses from the bats that are likely to transmit spontaneously the viruses to humans and other animals due to the specificity of their migration and habitat ( 23 , 24 ). In this study, we cloned Tadarida brasiliensis MDA5 for the first time, analyzed its function, explored the differences in bats’ innate immunity, and explained the coexistence of bats with viruses.…”

Section: Introductionmentioning

confidence: 99%

Bat Employs a Conserved MDA5 Gene to Trigger Antiviral Innate Immune Responses

et al. 2022

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Bats are important hosts for various zoonotic viral diseases. However, they rarely show signs of disease infection with such viruses. As the first line for virus control, the innate immune system of bats attracted our full attention. In this study, the Tadarida brasiliensis MDA5 gene (batMDA5), a major sensor for anti-RNA viral infection, was first cloned, and its biological functions in antiviral innate immunity were identified. Bioinformatics analysis shows that the amino acid sequence of batMDA5 is poorly conserved among species, and it is evolutionarily closer to humans. The mRNA of batMDA5 was significantly upregulated in Newcastle disease virus (NDV), avian influenza virus (AIV), and vesicular stomatitis virus (VSV)-infected bat TB 1 Lu cells. Overexpression of batMDA5 could activate IFNβ and inhibit vesicular stomatitis virus (VSV-GFP) replication in TB 1 Lu cells, while knockdown of batMDA5 yielded the opposite result. In addition, we found that the CARD domain was essential for MDA5 to activate IFNβ by constructing MDA5 domain mutant plasmids. These results indicated that bat employs a conserved MDA5 gene to trigger anti-RNA virus innate immune response. This study helps understand the biological role of MDA5 in innate immunity during evolution.

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“…Notably, bats also host major mammalian paramyxoviruses, including Nipah and Hendra viruses, members of the Henipavirus genus that are responsible for highly transmissible and lethal respiratory outbreaks. Recently, three new genera of orthoparamyxoviruses have been defined by the ICTV ( https://talk.ictvonline.org/taxonomy/ ), i.e., Jeilongvirus, Narmovirus, and Salemvirus , while hundreds of unclassified “bat paramyxoviruses” are being uploaded to the GenBank database and increasingly reported worldwide [ 57 – 59 ]. A cluster of bat paramyxoviruses has been proposed as a new genus named Shaanvirus [ 46 ], and together with the genus Jeilongvirus , they encompass a large number of bat- and rodent-associated viruses.…”

Section: Discussionmentioning

confidence: 99%

The virome of the white-winged vampire bat Diaemus youngi is rich in circular DNA viruses

et al. 2022

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In the Neotropical region, the white-winged vampire bat ( Diaemus youngi ) is the rarest of the three species of vampire bats. This bat species feeds preferentially on bird blood, and there is limited information on the viruses infecting D. youngi . Hence, this study aimed to expand the knowledge about the viral diversity associated with D. youngi by sampling and pooling the lungs, liver, kidneys, heart, and intestines of all animals using high-throughput sequencing (HTS) on the Illumina MiSeq platform. A total of three complete and 10 nearly complete circular virus genomes were closely related to gemykrogvirus ( Genomoviridae family), smacovirus ( Smacoviridae family), and torque teno viruses (TTVs) ( Anelloviridae family). In addition, three sequences of bat paramyxovirus were detected and found to be closely related to viruses reported in Pomona roundleaf bats and rodents. The present study provides a snapshot of the viral diversity associated with white-winged vampire bats and provides a baseline for comparison to viruses detected in future outbreaks. Supplementary Information The online version contains supplementary material available at 10.1007/s11262-022-01897-6.

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Detection of multiple viruses in oropharyngeal samples from Brazilian free-tailed bats (Tadarida brasiliensis) using viral metagenomics

Cited by 20 publications

References 30 publications

Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

Bat Employs a Conserved MDA5 Gene to Trigger Antiviral Innate Immune Responses

The virome of the white-winged vampire bat Diaemus youngi is rich in circular DNA viruses

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