Detection of Mycobacterium bovis infection in skin test-negative cattle with an assay for bovine interferon-gamma

Neill, S.D.; Cassidy, Joseph P.; Hanna, J; Mackie, D.P.; Pollock, J.M.; Clements, Archie C. A.; Walton, Emma Louise; Bryson, D.G.

doi:10.1136/vr.135.6.134

Cited by 100 publications

(74 citation statements)

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“…value and to reduce the number of false-negative animals, which could introduce the bovine tuberculosis into a new area (Snider 1982, Neill et al 1994, Whipple et al 1995, Archetti et al 1996, Llamazares et al 1999, Cagiola et al 2004, Vordermeier et al 2004. The use of the INFγ assay as a conϐirmatory test is also advisable by the simulations done, which showed a very high speciϐicity rate when serial testing with the two assays were used (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil

Lopes

Alves²,

Stynen

et al. 2012

Pesq. Vet. Bras.

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This study aimed to evaluate the interference of tuberculin test on the gamma-interferon (INFg) assay, to estimate the sensitivity and specificity of the INFg assay in Brazilian conditions, and to simulate multiple testing using the comparative tuberculin test and the INFg assay. Three hundred-fifty cattle from two TB-free and two TB-infected herds were submitted to the comparative tuberculin test and the INFg assay. The comparative tuberculin test was performed using avian and bovine PPD. The INFg assay was performed by the BovigamTM kit (CSL Veterinary, Australia), according to the manufacturer's specifications. Sensitivity and specificity of the INFg assay were assessed by a Bayesian latent class model. These diagnostic parameters were also estimate for multiple testing. The results of INFg assay on D0 and D3 after the comparative tuberculin test were compared by the McNemar's test and kappa statistics. Results of mean optical density from INFg assay on both days were similar. Sensitivity and specificity of the INFg assay showed results varying (95% confidence intervals) from 72 to 100% and 74 to 100% respectively. Sensitivity of parallel testing was over 97.5%, while specificity of serial testing was over 99.7%. The INFg assay proved to be a very useful diagnostic method.

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Section: Discussionmentioning

confidence: 99%

Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil

Lopes

Alves²,

Stynen

et al. 2012

Pesq. Vet. Bras.

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“…In most of them, the sensitivity of the IFN-γ test was higher compared to the tuberculin tests [13,18,24,28,32,44,45]. The specificity of the IFN-γ assay was 90.6 to 98.6% [3,20,44].…”

Section: Introductionmentioning

confidence: 99%

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

et al. 2006

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-The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-γ) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-γ assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-γ test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-γ test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-γ tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds. tuberculosis / paratuberculosis / IDTB / IFN-γ / eradication

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“…As this is an in vitro test that has the advantage of not interfering with the immune status of the animal and may be repeated in the same animal is the need to respect the period of desensitization. This assay showed the increase in the sensitivity and the possibility of more rapid repeat testing, no need for a second visit to the farm and more objective test procedures and interpretation in comparison to the TST (Faye et al, 2011;Neill et al, 1994;Schiller et al, 2010;Wood and Jones, 2001).…”

Section: Gamma Interferon Assays (Bovigam)mentioning

confidence: 99%

Diagnosis of bovine tuberculosis: review of main techniques

2015

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Bovine tuberculosis (BTB) remains an important economic and zoonotic problem in Latin America. Traditionally, the fight against BTB is initiated by the implementation of routine diagnostic tests for certification of free properties. The diagnosis of BTB can be made by direct and indirect methods, in which we can mention clinical, post mortem, histopathological, immunological, bacteriological and molecular methods. The renewal of scientific interest in tuberculosis in recent year has led to develop and improve methods of diagnosis, prevention, control and eradication of BTB. The aim of this review is to present and discuss different diagnosis methods of BTB.Keywords: tuberculosis bovine, diagnosis, Mycobacterium bovis, new techniques. Diagnóstico de tuberculose bovina: revisão das principais técnicas ResumoA tuberculose bovina (BTB) continua sendo um importante problema econômico na América Latina, com potenciais consequências zoonóticas. Tradicionalmente, a luta contra a tuberculose bovina tem sido iniciada pela execução de testes de diagnóstico de rotina para a certificação de propriedades livres da doença. O diagnóstico de BTB pode ser feito através de métodos diretos e indiretos, nos quais podemos citar os métodos clínicos, post mortem, histopatológicos, imunológicos, bacteriológicos e moleculares. A renovação do interesse científico em tuberculose nos últimos anos tem levado à necessidade de desenvolver e melhorar os métodos de diagnóstico, prevenção, controle e erradicação da BTB. O objetivo deste artigo é analisar e discutir sobre os diferentes métodos de diagnóstico de BTB.Palavras-chave: tuberculose bovina, diagnóstico, Mycobacterium bovis, novas técnicas.

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Detection of Mycobacterium bovis infection in skin test-negative cattle with an assay for bovine interferon-gamma

Cited by 100 publications

References 0 publications

Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil

Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

Diagnosis of bovine tuberculosis: review of main techniques

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