Detection of Mycobacterium tuberculosis Complex in Lung Specimen of Slaughtered Cattle and Goats by a DNA Based multiplex Polymerase Chain Reaction and Ziehl-Neelsen Methods In Jos, Nigeria

Chukwu, I. D.; Chukwu, C. O. O.; Kandakai-Olukemi, YT; Owolodun, Olajide Adewale; Nwosuh, Chika; Agada, G. O. A.; Audu, B. J.; Chollom, S. C.

doi:10.9734/bmrj/2013/3708

Cited by 5 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, tubercle bacilli were isolated from cattle with no visible lesions suggesting that M. bovis can be detected from cattle with or without lesions (Ku et al, 2018). The proportion of samples positive by acid-fast staining (16.4%) was low as compared to the about 30% reported from a different part of Nigeria (Chukwu et al, 2013), and also lower than the 58% reported elsewhere in Africa (Koro et al, 2013). This may be related to the adjusted concentration of acid-alcohol solution (5% instead of the regular 3%) and extended decolorization time of 10 min employed during the staining procedure in the present study.…”

Section: Discussionmentioning

confidence: 79%

Survey of bovine tuberculosis in Nigerian beef cattle

Ahmad¹,

Kudi²,

Abdulkadir³

et al. 2019

Open Vet J.

View full text Add to dashboard Cite

Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) that primarily affects cattle, but also other domestic and wild mammals. In Nigeria, abattoir monitoring of gross bTB lesions is the only control method being applied in all animals. This study aims to investigate tubercle bacilli infection in slaughtered cattle found with visible tuberculosis-like lesions. Lesions suggesting bTB were detected in 226 cattle during abattoir monitoring in Zamfara State, Nigeria. Tissue samples collected from the affected carcasses were subjected to Ziehl-Neelsen stain (ZN). Of the 226 carcasses with lesions, 37 (16.4%) were positive by the Ziehl-Neelsen stain (ZN), and MTBC was detected from 34 (91.9%) of the 37 ZN-positive samples. Molecular typing by region of difference (RD) deletion analysis revealed the genotype of Mycobacterium bovis, Mycobacterium caprae and Mycobacterium tuberculosis. Infection was most significantly associated with age of the animals (OR = 3.49; CI: 1.29-9.47 [p = 0.002]). The findings indicate a serious threat for health as well as for TB control in Nigeria.

show abstract

Section: Discussionmentioning

confidence: 79%

Survey of bovine tuberculosis in Nigerian beef cattle

Ahmad¹,

Kudi²,

Abdulkadir³

et al. 2019

Open Vet J.

View full text Add to dashboard Cite

show abstract

“…Despite, the large number of livestock population in the region, there is no information on BTB. Despite the fact that bovine tuberculosis is a public health threat and also leads to economic losses, in Ethiopia research on and control of animal tuberculosis has not received much attention like human tuberculosis (Chukwu et al, 2013).Thus the present study was designed to determine the prevalence of bovine tuberculosis in Gambella town municipal abattoir and identifying risk factors associated with bovine tuberculosis, to isolate and molecular characterization of mycobacterial isolates from slaughtered cattle and from human pulmonary TB patients and to investigate the potential risk factors for zoonotic transmission of mycobacterial infections.…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Bovine Tuberculosis in Cattle and its Public Health Implications in Gambella Region, Ethiopia

Alemu¹,

Mamo²,

Ameni³

et al. 2016

mmr

View full text Add to dashboard Cite

A cross sectional study was conducted from December 2014 to May 2015 in Gambella town municipal abattoir and health centers to investigate the prevalence of bovine tuberculosis, isolation and molecular characterization of its causative agents, and to assess its public health implications in Gambella, Ethiopia. Postmortem examination, bacteriological culturing, RD deletion typing, and spoligotyping were used for investigation. The overall prevalence of bovine tuberculosis in cattle was 13.2% (66/500) on the basis of detailed postmortem examination. Statistical significant difference was observed in the prevalence of bovine tuberculosis among different body conditioned animals (χ2 = 39.105, P=0.000 and breeds (χ2 = 24.996, P=0.000). Molecular characterization of 11 mycobacterial isolates from human patients using RD9 deletion typing showed that all were M. tuberculosis, and further spoligotyping of the isolates revealed that SIT289, SIT134, SIT1634, SIT142 and one new strain (not found in the spoligotype databases). Of these M. tuberculosis strains identified, SIT 289 and SIT134 were found in cluster with 45.5% (5/11) cluster rate. Lineage of the human isolates indicated that 27.3% (3/11) were in Euro-American, and 9.1% (1/11) Indo-oceanic family in TB-insight database. Interestingly, one isolate from animal taken from mediastinal lymph node was confirmed to be M. tuberculosis using RD4 deletion typing and spoligotyping, in which the isolate was identified as SIT523 with Indo-oceanic lineage family. Awareness of cattle owners for bovine tuberculosis was found insufficient (22%), and the results also revealed the presence of potential risk factor for zoonotic transmission. In conclusion, isolation of M. tuberculosis in cattle, and occurrence of various strains of M. tuberculosis in the communities warrants further systematic investigation on the transmission of the disease in Gambella region of Ethiopia.

show abstract

“…Te prevalence in intensive dairy herds varied from 22 to 47% [12], with a prevalence of 1.1%-24.7% in abattoirs and 3.5-50% in crossbreed farms [13][14][15]. Unlike human TB, it has not received more focus on research, and several factors impair disease control and eradication, along with the limited sensitivity of diagnostic tests and slaughter control strategies not applied due to economic constraints, but their efectiveness is applicable in developed countries [16]. Due to inadequate comprehensive abattoir surveillance and a lack of diagnostic facilities, the BTB has limited information [10,17], particularly on the genotypic characteristics of M. bovis, a strain afecting the cattle population in Ethiopia [18].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Identification of Mycobacterium bovis from Slaughtered Cattle in Nekemte Municipality Abattoir, Ethiopia

Mezgebu Gemeda,

Tola,

Donde

et al. 2023

Veterinary Medicine International

View full text Add to dashboard Cite

Tuberculosis (TB) is a zoonotic disease that can spread from animals to humans as well as from human to human. Little research has been conducted on bovine tuberculosis prevalence and molecular characterization in the western part of Ethiopia. To investigate this, a cross-sectional study was conducted on slaughtered cattle at the Nekemte municipal abattoir between January 2020 and June 2021. A detailed postmortem examination, culture, acid-fast staining technique, molecular characterization using RD4 deletion, and spoligotyping were all carried out. Based on a detailed postmortem examination, the overall prevalence of bovine tuberculosis was 7.8% (80 of 1020). Mycobacterium isolation confirmed only 12.5% (10/80) of the suspected tuberculosis tissue lesions. With acid-fast bacilli staining, all Mycobacterium spp. isolates (n = 10) were positive. However, only 9/10 isolates were confirmed to be M. bovis with RD4 molecular deletion typing. Spoligotyping revealed that 55.6% (5/9) of the isolate patterns had previously been reported, but 44.4% (4/9) of the isolates were new. In the current investigation, it was discovered that 80% (4/5) of the M. bovis strains circulating in the cattle population of study regions were SB2233 (2/5) and SB0134 (2/5), whereas 20% (1/5) of the strains corresponded to SB1176, which is compatible with previously documented M. bovis spoligotypes. These findings suggested that M. bovis was the main cause of bovine tuberculosis in the study area and posed a risk of disease transmission from cattle to humans due to low levels of public health awareness. As such, improved awareness among citizens and the development of control policies are warranted.

show abstract

Detection of Mycobacterium tuberculosis Complex in Lung Specimen of Slaughtered Cattle and Goats by a DNA Based multiplex Polymerase Chain Reaction and Ziehl-Neelsen Methods In Jos, Nigeria

Cited by 5 publications

References 8 publications

Survey of bovine tuberculosis in Nigerian beef cattle

Survey of bovine tuberculosis in Nigerian beef cattle

Molecular Epidemiology of Bovine Tuberculosis in Cattle and its Public Health Implications in Gambella Region, Ethiopia

Isolation and Molecular Identification of Mycobacterium bovis from Slaughtered Cattle in Nekemte Municipality Abattoir, Ethiopia

Contact Info

Product

Resources

About