Detection of Mycobacterium tuberculosis Genome in Vitreous Fluid of Eyes with Multifocal Serpiginoid Choroiditis

Controversies in ocular tuberculosis

“…Recent advances in diagnostic tools for OTB such as molecular techniques for MTB DNA detection and immunological tests such as IGRA have improved the specificity of making this diagnosis 68 69. However, the clinical diagnosis of OTB remains a complex issue, as these investigations are mainly adjunctive and complementary, while the clinical manifestations of OTB are protean 53.…”

Epidemiology and clinical features of inflammatory retinal vascular occlusions: pooled data from two tertiary‐referral institutions

“…Among patients with TB, the tuberculin skin test (Mantoux test) was positive (≥10‐mm induration at 48–72 h) in 24 patients (24/33; 72.73%) (possible intraocular TB). Among the remaining nine patients, QuantiFERON TB Gold was positive in seven patients (probable intraocular TB), and two eyes tested positive for TB using the multi‐targeted polymerase chain reaction (PCR) assay (Gene Xpert MTB/RIF assay, Cepheid, Sunnyvale, CA, USA) (confirmed intraocular TB) . Patients with SLE were already diagnosed prior to ophthalmic complaints by the internists on the basis of clinical and laboratory data (American College of Rheumatology guidelines) .…”

MTBDRplus for the rapid diagnosis of ocular tuberculosis and screening of drug resistance

“…Further, unlike our study, none of these studies have confirmed their results by carrying out gene sequencing for the offending drug. Two other studies, 15,24 comparing results of MTBDRplus assay version 2.0 with sequencing have reported a sensitivity of 96.7 and 100%, respectively, for detecting rifampicin resistance and 100% each for detecting isoniazid resistance. The present study differs from these two by the fact that while the former was carried out on 115 culture-positive undefined (PTB or EPTB) samples, the latter was conducted on mere eleven VF samples from cases of IOTB.…”

“…MTBDRplus line probe assay is a commercially available diagnostic technique that is currently recommended for smear positive pulmonary specimen and from culture. 14 To the best of our knowledge, there are only two studies 15,16 addressing the role of MTBDRplus assay, on 11 and five cases, respectively, for the diagnosis of IOTB and detection of resistance simultaneously. We, therefore, evaluated the diagnostic potential of MTBDRplus assay on substantial number of vitreous fluid samples to add to the existing literature in this field.…”