Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina

Tamiozzo, Pablo; Estanguet, Abel; Maito, Julia; Tirante, Liliana; Pol, Martín; Giraudo, José

doi:10.1016/s0325-7541(14)70059-8

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…The group decided to perform the extraction by this method, followed by a protocol of DNA purification to obtain a better quality analytical material, taking into account the costs and practicality of the method. For conventional PCR, it was possible to use thermolysis method with satisfactory results in detecting M. canadense and M. californicum from isolates of milk samples (Tamiozzo et al 2014).…”

Section: Molecular Analysismentioning

confidence: 99%

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

et al. 2020

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The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil.

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Section: Molecular Analysismentioning

confidence: 99%

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

et al. 2020

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“…Amplified PCRs products based on ITS sequences have distinguished 55 bacterial species, including 18 Clostridium and 15 Mycoplasma [66]. More recently, rRNA ITS, specifically 16S-23S, has been used in Vibrio identification [67], Mycoplasma from cattle [68], Brucella [69,70], and Mycobacteria [71,72].…”

Section: Molecular Markersmentioning

confidence: 99%

Pathogenomics and Molecular Advances in Pathogen Identification

Quiroz-Castañeda¹

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

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Today exists a spread spectrum of tools to be used in pathogen identification. Traditional staining and microscopic methods as well as modern molecular methods are presented in this chapter. Pathogen identification is only the beginning to obtain information related to pathogenicity of the microorganism in the near future. Once the pathogen is identified, genome-sequencing methods will provide a significant amount of information that can be elucidated only through bioinformatics methods. In this point, pathogenomics is a powerful tool to identify potential virulence factors, pathogenicity islands, and many other genes that could be used as therapeutic targets or in vaccine development. In this chapter, we present an update of the molecular advances used to identify pathogens and to obtain information of their diversity. We also review the most recent studies on pathogenomics with a special attention on pathogens of veterinary importance.

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“…uberis sod A, cpn60 yqiL ., hasA , [27] , [85] E. coli 16S rRNA enterobacterial repetitive intergenic consensus (ERIC). [86] Mycoplasma 16 S –23 S rRNA p40 - MLST of fusA, gyrB, lepA and rpoB [25] , [87] , [88] …”

Section: Molecular Markers Of Infectious Mastitis Inducers: Identificmentioning

confidence: 99%

Molecular biological tools applied for identification of mastitis causing pathogens

El-Sayed

Awad

Abdou

et al. 2017

International Journal of Veterinary Science and Medicine

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The molecular diagnostic tools became the gold standard of mastitis diagnosis in the last few years. They enable rapid, qualitative, quantitative and large scale diagnosis. In addition to their role in diagnosis, they can identify pathogens at the subspecies level which is necessary for the epidemiological studies. They are increasingly used in mastitis control programs through identification of suitable candidates for vaccine production and through the selection of mastitis resistant cattle breeds. The present molecular techniques are continuously improved and new techniques are developed in order to provide higher sensitivity and specificity and to minimize the costs. The present work aims to provide an overview of the modern molecular tools, discuss why they replaced the traditional tools and became the new gold standard in mastitis diagnosis through comparing both traditional and molecular tools, explore the prospective of the molecular diagnostic techniques in mastitis diagnosis and control and to explore new horizons of using molecular assays in near future.

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Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina

Cited by 6 publications

References 9 publications

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis

Pathogenomics and Molecular Advances in Pathogen Identification

Molecular biological tools applied for identification of mastitis causing pathogens

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