Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Tourova, T. P.; Соколова, Д. Ш.; Семенова, Е. М.; Шумкова, Е. С.; Korshunova, A. V.; Бабич, Т. Л.; Полтараус, А. Б.; Назина, Т. Н.

doi:10.1134/s0026261716060199

Cited by 45 publications

(23 citation statements)

References 27 publications

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“…The 16S rRNA, gyrB and parE gene sequences revealed in the genome had 100%, 100% and 99.9% similarity, respectively, with those determined earlier for the strain 1017 [5]. The results of phenotypic studies characterizing the strain as an aerobic spore-forming bacterium utilizing a wide range of organic substrates were confirmed by detection of respective genes.…”

Section: Experimental Design Materials and Methodssupporting

confidence: 67%

“…The strain utilizes fatty acids, sugars, amino acids, aromatic compounds, and n -alkanes of crude oil. The 16S rRNA gene sequence of strain 1017 has 99.5% similarity with the respective gene of Geobacillus kaustophilus R-35646 (FN428694) [5]. Analysis of the gyrB and parE genes of the new strain 1017 revealed 96.0% and 97% similarity to the relevant genes of G .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 95%

“…The strain 1017 (= VKM B-3132) was isolated from a production water sample of the Dagang high-temperature heavy oil reservoir (Hebei Province, China) (38°40′4.7″ N, 117°22′38.0″ E) [4], [5]. Strain 1017 is an aerobic endospore-forming bacterium, motile during the early exponential growth phase, and able to grow within the temperature range from 38 to 68 °C with an optimum at 60 °C.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Genome sequencing and annotation of Geobacillus sp. 1017, a hydrocarbon-oxidizing thermophilic bacterium isolated from a heavy oil reservoir (China)

et al. 2017

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The draft genome sequence of Geobacillus sp. strain 1017, a thermophilic aerobic oil-oxidizing bacterium isolated from formation water of the Dagang high-temperature oilfield, China, is presented here. The genome comprised 3.6 Mbp, with the G + C content of 51.74%. The strain had a number of genes responsible for numerous metabolic and transport systems, exopolysaccharide biosynthesis, and decomposition of sugars and aromatic compounds, as well as the genes related to resistance to metals and metalloids. The genome sequence is available at DDBJ/EMBL/GenBank under the accession no MQMG00000000. This genome is annotated for elucidation of the genomic and phenotypic diversity of new thermophilic alkane-oxidizing bacteria of the genus Geobacillus.

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Section: Experimental Design Materials and Methodssupporting

confidence: 67%

Section: Experimental Design Materials and Methodsmentioning

confidence: 95%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Genome sequencing and annotation of Geobacillus sp. 1017, a hydrocarbon-oxidizing thermophilic bacterium isolated from a heavy oil reservoir (China)

et al. 2017

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“…Geobacillus and Aeribacillus [34,36] In addition to the AlkB system, two other oxygenases have been reported in mesophiles. These are cytochrome P450 monooxygenases, which belong to the cytochrome CYP153 family [75], and flavin-dependent dioxygenases, encoded by the almA gene [76].…”

Section: N-alkanesmentioning

confidence: 99%

“…[ST9]. Similar to AlmA, LadA is a flavin-dependent monooxygenase of the family of bacterial luciferases, and is responsible for the degradation of long-chain n-alkanes C15-C36.Recently, the simultaneous presence of alkB and ladA genes has been reported in two strains of Geobacillus and one of Aeribacillus[36] [ST12].In summary, both mesophiles and thermophiles express AlkB monooxygenase enzymes; however, in addition, mesophiles have CYP153 and AlmA enzymes, while thermophiles express LadA monooxygenases.3.3.2. MAHsPhenol has been the most studied MAH in thermophiles.…”

mentioning

confidence: 99%

Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives

Nzila

2018

Preprint

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Contamination of the environment by petroleum products is a growing concern worldwide, and strategies to remove these contaminants have been evaluated. One of these strategies is biodegradation, which consists of the use of microorganisms. Biodegradation is significantly improved by increasing the temperature of the milieu, thus, the use of thermophiles, microbes that thrive in high-temperature environments, will render this process more efficient. For instance, various thermophilic enzymes have been used in industrial biotechnology because of their unique catalytic properties. Biodegradation has been extensively studied in the context of mesophilic microbes, and the mechanisms of biodegradation of aliphatic and aromatic petroleum hydrocarbons have been elucidated. However, in comparison, little work has been carried out on the biodegradation of petroleum hydrocarbons by thermophiles. In this paper, a detailed review of the degradation of petroleum hydrocarbons (both aliphatic and aromatic) by thermophiles has been carried out. This work has identified the characteristics of thermophiles, and unravelled specific catabolic pathways of petroleum products that are only found in thermophiles. Gaps that limit our understanding of the activity of these microbes have also been highlighted, and finally, different strategies that can be used to improve the efficiency of degradation of petroleum hydrocarbons by thermophiles have been proposed.

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Prokaryotic Hydrocarbon Degraders

Prince¹,

Amande

McGenity

2019

Taxonomy, Genomics and Ecophysiology of Hydrocarbon-Degrading Microbes

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Detection of n-alkane biodegradation genes alkB and ladA in thermophilic hydrocarbon-oxidizing bacteria of the genera Aeribacillus and Geobacillus

Cited by 45 publications

References 27 publications

Genome sequencing and annotation of Geobacillus sp. 1017, a hydrocarbon-oxidizing thermophilic bacterium isolated from a heavy oil reservoir (China)

Genome sequencing and annotation of Geobacillus sp. 1017, a hydrocarbon-oxidizing thermophilic bacterium isolated from a heavy oil reservoir (China)

Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives

Prokaryotic Hydrocarbon Degraders

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