Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Danh, Kenneth; Karp, Donna Grace; Singhal, Malvika; Tankasala, Akshaya; Gebhart, David; Cortez, Felipe de Jesus; Tandel, Devangkumar; Robinson, Peter V.; Seftel, David; Stone, Mars; Simmons, Graham; Bagri, Anil; Schreiber, Martin A.; Buser, Andreas; Holbro, Andreas; Battegay, Manuel; Hanson, Carl V.; Mills, John R.; Granger, Dane; Theel, Elitza S.; Stubbs, James R.; Corash, Laurence; Tsai, Cheng‐ting

doi:10.1038/s41467-022-31796-1

Cited by 10 publications

(14 citation statements)

References 34 publications

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“…Seven powerfully neutralizing antibodies are described by Cerutti et al in cryo-EM and crystal structures in complex with either the SARS-CoV-2 spike or NTD ( 38 ). Also, Danh et al shows that S1 neutralization signals are stronger than RBD in the ACE2 neutralization assay ( 39 ). This phenomenon might be explained by the possibility that NAbs could neutralize by attaching to a nearby epitope on the S1 protein outside of the RBD but still prevent binding to ACE2 due to the stearic hindrance.…”

Section: Discussionmentioning

confidence: 99%

Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Kuo¹,

Kuo²,

Du³

et al. 2023

Molecular & Cellular Proteomics

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Section: Discussionmentioning

confidence: 99%

Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Kuo¹,

Kuo²,

Du³

et al. 2023

Molecular & Cellular Proteomics

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“…Several complimentary assays were used to characterize antibody levels, neutralizing capacity, and the spectrum of antigen reactivity as previously reported 10 . The levels of antibodies to the S and N antigens were measured by agglutination‐dependent polymerase chain reaction (ADAP) 17 . The spectrum of antibody reactivity to coronavirus antigens and other respiratory virus antigens was assessed by coronavirus antigen microarrays (COVAM) 18 .…”

Section: Study Design and Methodsmentioning

confidence: 99%

“…10 The levels of antibodies to the S and N antigens were measured by agglutination-dependent polymerase chain reaction (ADAP). 17 The spectrum of antibody reactivity to coronavirus antigens and other respiratory virus antigens was assessed by coronavirus antigen microarrays (COVAM). 18 The detection of neutralizing activity to the soluble ACE-2 receptor was performed by an ADAP inhibition assay.…”

Section: Evaluation Of Antibodies To Sars-cov-2 In Donor Plasma and L...mentioning

confidence: 99%

“…18 The detection of neutralizing activity to the soluble ACE-2 receptor was performed by an ADAP inhibition assay. 17 The COVAM analysis including mean fluorescence intensity (MFI) normalization, unsupervised cluster analysis, and principal component analysis were performed as previously described. 10,18 The primary comparison was for activities after collection prior to pathogen reduction and after lyophilization of individual CCPs and for activities of the pooled lyophilized CCP.…”

Section: Evaluation Of Antibodies To Sars-cov-2 In Donor Plasma and L...mentioning

confidence: 99%

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Characterization of antibodies to SARS‐CoV‐2 in lyophilized plasma prepared with amotosalen‐UVA pathogen reduction

Martinaud,

Bagri,

Tsai

et al. 2023

Transfusion

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BackgroundSevere acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2)‐infected patients exhibit disease ranging from asymptomatic to severe pneumonia, multi‐organ failure, and death. convalescent COVID plasma (CCP) from recovered patients with high levels of neutralizing antibodies has demonstrated therapeutic efficacy to reduce the morbidity of coronavirus disease 2019 (COVID‐19) in some studies. The development of assays to characterize the activity of CCP to neutralize SARS‐CoV‐2 infectivity offers the possibility to improve potential therapeutic efficacy. Lyophilization of CCP may increase the availability of this therapy. We hypothesized that SARS‐CoV‐2 antibody profiles of pooled lyophilized pathogen‐reduced CCP from COVID‐19‐recovered blood donors retains virus‐neutralizing efficacy as reported for frozen pathogen‐reduced CCP.MethodsPooled lyophilized pathogen‐reduced plasma was prepared from recovered COVID plasma donors. Antibodies to SARS‐CoV‐2 were characterized in each donor plasma prior to pathogen reduction and lyophilization and after lyophilization of individual CCP, and in the lyophilized CCP pool. Several complimentary assays were used to characterize antibody levels, neutralizing capacity, and the spectrum of antigen reactivity. The mean values for individual plasma samples and the value in the pool were compared.ResultsThe mean ratio for antibody binding to SARS‐CoV‐2 antigens before and after treatment was 0.95 ± 0.22 mean fluorescent intensity (MFI) units. Antibody activity to an array of influenza virus antigens demonstrated a mean activity ratio of 0.92 ± 0.12 MFI before and after treatment.ConclusionsThe antibody activity in pooled pathogen‐reduced lyophilized CCPs demonstrated minimal impact due to pathogen reduction treatment and lyophilization.

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“…To date, a large variety of biochemical methodologies, such as polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), colorimetric and luminescent assays, have been established for the qualitative and quantitative analysis of circulating biomolecules [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Generally, the average response of a large amount of analytes is measured as an assay signal.…”

Section: Introductionmentioning

confidence: 99%

Single-Particle Optical Imaging for Ultrasensitive Bioanalysis

Liu

et al. 2022

Biosensors

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The quantitative detection of critical biomolecules and in particular low-abundance biomarkers in biofluids is crucial for early-stage diagnosis and management but remains a challenge largely owing to the insufficient sensitivity of existing ensemble-sensing methods. The single-particle imaging technique has emerged as an important tool to analyze ultralow-abundance biomolecules by engineering and exploiting the distinct physical and chemical property of individual luminescent particles. In this review, we focus and survey the latest advances in single-particle optical imaging (OSPI) for ultrasensitive bioanalysis pertaining to basic biological studies and clinical applications. We first introduce state-of-the-art OSPI techniques, including fluorescence, surface-enhanced Raman scattering, electrochemiluminescence, and dark-field scattering, with emphasis on the contributions of various metal and nonmetal nano-labels to the improvement of the signal-to-noise ratio. During the discussion of individual techniques, we also highlight their applications in spatial–temporal measurement of key biomarkers such as proteins, nucleic acids and extracellular vesicles with single-entity sensitivity. To that end, we discuss the current challenges and prospective trends of single-particle optical-imaging-based bioanalysis.

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Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Cited by 10 publications

References 34 publications

Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Characterization of antibodies to SARS‐CoV‐2 in lyophilized plasma prepared with amotosalen‐UVA pathogen reduction

Single-Particle Optical Imaging for Ultrasensitive Bioanalysis

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