Detection of Nitric Oxide and Peroxynitrite in Biological Systems: A State-of-the-Art Review

Hogg, Neil; Zielonka, Jacek; Kalyanaraman, Balaraman

doi:10.1016/b978-0-12-804273-1.00003-x

Cited by 12 publications

(11 citation statements)

References 120 publications

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“…High-resolution images were taken using a meta laser scanning confocal microscope (LSM-510; Carl Zeiss). Due to the controversy raised on the specificity of biotin switch assay [33,34], quantitative chemiluminescence-based SNO measurement was done and additional controls with DTT, ascorbate and GSNO + cysteine for biotin switch assay were performed.…”

Section: Methodsmentioning

confidence: 99%

Keap1 controls protein S-nitrosation and apoptosis-senescence switch in endothelial cells

et al. 2020

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Premature senescence, a death escaping pathway for cells experiencing stress, is conducive to aging and cardiovascular diseases. The molecular switch between senescent and apoptotic fate remains, however, poorly recognized. Nrf2 is an important transcription factor orchestrating adaptive response to cellular stress. Here, we show that both human primary endothelial cells (ECs) and murine aortas lacking Nrf2 signaling are senescent but unexpectedly do not encounter damaging oxidative stress. Instead, they exhibit markedly increased S-nitrosation of proteins. A functional role of S-nitrosation is protection of ECs from death by inhibition of NOX4-mediated oxidative damage and redirection of ECs to premature senescence. S-nitrosation and senescence are mediated by Keap1, a direct binding partner of Nrf2, which colocalizes and precipitates with nitric oxide synthase (NOS) and transnitrosating protein GAPDH in ECs devoid of Nrf2. We conclude that the overabundance of this “unrestrained” Keap1 determines the fate of ECs by regulation of S-nitrosation and propose that Keap1/GAPDH/NOS complex may serve as an enzymatic machinery for S-nitrosation in mammalian cells.

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Section: Methodsmentioning

confidence: 99%

Keap1 controls protein S-nitrosation and apoptosis-senescence switch in endothelial cells

et al. 2020

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“…It is widely accepted that those oxidants are involved in different physiological and pathophysiological processes, but the detection and quantitation of those species remains a challenging task, mainly due to their high reactivity, short lifetime, and very low steady-state concentrations in biological systems. For that reason, fluorescent or chemiluminescent probes are widely used to study the role of biological oxidants in physiological and pathophysiological processes (Wardman, 2007 ; Winterbourn, 2014 ; Kalyanaraman et al, 2016 , 2017 ; Hogg et al, 2017 ; Hardy et al, 2018 ). Several molecular probes that are oxidized to easily detectable and relatively stable fluorescent products have been developed, making possible real-time monitoring of the formation of biological oxidants.…”

Section: Introductionmentioning

confidence: 99%

Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology

et al. 2020

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Boronate-based molecular probes are emerging as one of the most effective tools for detection and quantitation of peroxynitrite and hydroperoxides. This review discusses the chemical reactivity of boronate compounds in the context of their use for detection of biological oxidants, and presents examples of the practical use of those probes in selected chemical, enzymatic, and biological systems. The particular reactivity of boronates toward nucleophilic oxidants makes them a distinct class of probes for redox biology studies. We focus on the recent progress in the design and application of boronate-based probes in redox studies and perspectives for further developments.

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“…H 2 O 2 decomposes into reactive oxygen species (hydroxyl radicals, OH and superoxide ions, O 2 − ) which attack important biomolecules that create cellular/viral structure and function ( Linley et al, 2012 ). Moreover, the superoxide ion may combine with nitric oxide (NO) (in vivo) to form the peroxynitrite ion which can lead to lipid peroxidation, protein oxidation and inactivation of microbial enzymes ( Hogg et al, 2017 , Trujillo et al, 2010 ). Studies have suggested that the VHP displays a better ability to degrade protein structure ( Fichet et al, 2007 ).…”

Section: Proposed Hybrid Decontamination System (Hds)mentioning

confidence: 99%

Conceptualizing a novel Hybrid Decontamination System (HDS) based on UV/H2O2 treatment for the enhanced decontamination and reuse of N95 FFRs

Anand

Mahajan

Kataki

et al. 2021

Environmental Challenges

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The ongoing Pandemic of COVID-19 caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has severely stressed the worldwide healthcare system and has created dangerous shortages of personal protective equipment (PPE) including N95 filtering facepiece respirators (FFRs). Even though suppliers struggled to meet global demand for N95 masks at an unprecedented level, a shortage of FFR appears as a significant factor in the transmission of the disease to frontline workers. CDC, USA has mentioned that FFR decontamination and reuse may be necessary during times of shortage to ensure guaranteed availability. Hence present stressed condition faced by the healthcare sector seeks for an affordable decontamination strategy that can be replicated easily broadening the utility of FFR decontamination across a range of healthcare settings. After reviewing available literature on the various disinfection techniques that may be used for the decontamination of FFRs, a first of its kind, portable hybrid decontamination system/procedure has been conceptualized and designed. This system combines the disinfecting properties of both vaporous hydrogen peroxide (VHP) and ultra-violet C irradiation (UV C) to ensure maximum decontamination of N95 respirators. The instrument will be equipped with a hydrogen peroxide chamber and UV light source. Sterilization of the FFRs will be done through treatment with VHP followed by UV light treatment. The proposed system will allow the user to completely sterilize the FFRs in a time-efficient manner.

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Detection of Nitric Oxide and Peroxynitrite in Biological Systems: A State-of-the-Art Review

Cited by 12 publications

References 120 publications

Keap1 controls protein S-nitrosation and apoptosis-senescence switch in endothelial cells

Keap1 controls protein S-nitrosation and apoptosis-senescence switch in endothelial cells

Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology

Conceptualizing a novel Hybrid Decontamination System (HDS) based on UV/H2O2 treatment for the enhanced decontamination and reuse of N95 FFRs

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