Detection of novel influenza A(H1N1) virus by real-time RT-PCR

Whiley, David M.; Bialasiewicz, Seweryn; Bletchly, Cheryl; Faux, Cassandra E.; Harrower, Bruce; Gould, Allan R.; Lambert, Stephen B.; Nimmo, Graeme R.; Nissen, Michael D.; Sloots, Theo P.

doi:10.1016/j.jcv.2009.05.032

Cited by 83 publications

(71 citation statements)

References 2 publications

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“…The first Australian case of influenza A(H1N1)pdm09 virus was confirmed in Brisbane on 9 May 2009,20 and virus circulation became rapidly widespread in all site cities 21…”

Section: Resultsmentioning

confidence: 99%

Safety and tolerability of a 2009 trivalent inactivated split‐virion influenza vaccine in infants, children and adolescents

Lambert

Chuk

Nissen

et al. 2013

Influenza Resp Viruses

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ObjectiveTo evaluate the safety of CSL's split‐virion inactivated trivalent 2009 Southern Hemisphere formulation influenza vaccine (TIV) in children.MethodsWe enrolled 1992 healthy children into three groups: Cohorts A, ≥6 months to <3 years; B, ≥3 years to <9 years; and C, ≥9 years to <18 years. Children received one or two doses of 0·25 ml (22·5 μg haemagglutinin) or 0·5 ml (45 μg) TIV, depending on age and prior vaccination history. We collected post‐vaccination solicited adverse event (AE) data (days 0–6), including fever (temperature: ≥37·5°C axilla, ≥38·0°C oral), unsolicited AEs (days 0–29) and serious AEs (SAEs) and new‐onset chronic illnesses (NOCIs; to day 180 after last vaccination).ResultsAt least one solicited AE was reported by 80%/78%/78% of children in Cohorts A, B and C, respectively. Systemic AEs were more common among Cohort A (72% of participants), and local AEs were more common among Cohort C (71% of participants). Fever was more common in younger cohorts, in influenza vaccine‐naïve children (29% of Cohort A receiving their first dose), and following first compared with second doses. Severe fever following a first dose prevented 20 participants receiving their second scheduled vaccine dose. A 7‐month‐old participant had a single uncomplicated febrile convulsion on the day of vaccination.ConclusionsNearly 80% of subjects reported at least one solicited AE following immunization. Fever prevalence was highest in vaccine‐naïve Cohort A participants, similar to other paediatric studies using CSL vaccine. Further research to understand fever‐related AEs in children following CSL's TIV is recommended.

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“…The first Australian case of influenza A(H1N1)pdm09 virus was confirmed in Brisbane on 9 May 2009,20 and virus circulation became rapidly widespread in all site cities 21…”

Section: Resultsmentioning

confidence: 99%

Safety and tolerability of a 2009 trivalent inactivated split‐virion influenza vaccine in infants, children and adolescents

Lambert

Chuk

Nissen

et al. 2013

Influenza Resp Viruses

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“…However, the use of multiple targets can reduce such limitations, and may serve as means to confirm positive results (Whiley et al 2009).…”

Section: Conventional and Real Time Reverse-transcriptase Polymerase mentioning

confidence: 99%

Diagnostic Methods of Influenza a From Clinical Specimens: A Critical Review of Literature

Bello¹,

Lima

Azevedo³

2015

VR&R

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“…Diagnostic assays for influenza infection are numerous and include, among others, culture and direct fluorescent antibody staining, 14 -17 conventional reverse transcription-PCR (RT-PCR) and real-time RT-PCR (rRT-PCR), 14,15,[17][18][19][20][21][22][23] microarrays, 5,15,16,19 and a large number of commercially available rapid diagnostic tests. 19,24,25 However, these assays have diverse ranges in sensitivity and specificity, depending on the nature of the sample and duration of illness.…”

mentioning

confidence: 99%

Diagnosis of Influenza from Respiratory Autopsy Tissues

Denison

Blau

Jost

et al. 2011

The Journal of Molecular Diagnostics

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Although formalin-fixed, paraffin-embedded tissues can be used for diagnosis, frozen tissues offer the best chance to make a postmortem diagnosis of influenza because these tissues possess nucleic acids that are less degraded and, as a consequence, provide longer sequence information than that obtained from fixed tissues. We also determined that testing of all available respiratory tissues is critical for optimal detection of influenza virus in postmortem tissues. ( J Mol

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Detection of novel influenza A(H1N1) virus by real-time RT-PCR

Cited by 83 publications

References 2 publications

Safety and tolerability of a 2009 trivalent inactivated split‐virion influenza vaccine in infants, children and adolescents

Safety and tolerability of a 2009 trivalent inactivated split‐virion influenza vaccine in infants, children and adolescents

Diagnostic Methods of Influenza a From Clinical Specimens: A Critical Review of Literature

Diagnosis of Influenza from Respiratory Autopsy Tissues

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