Detection of ochratoxin A (OTA) in coffee using chemiluminescence resonance energy transfer (CRET) aptasensor

Jo, Eun-Jung; Mun, Hyouyoung; Kim, Su-Ji; Shim, Won‐Bo; Kim, Min-Gon

doi:10.1016/j.foodchem.2015.07.152

Cited by 107 publications

(30 citation statements)

References 34 publications

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“…By using energy transfer, several reports reported were based on chemiluminescence resonance energy transfer (CRET) [83] or FRET [84]. For example, using the intrinsic fluorescence properties of anti-OTA antibody and OTA, a label-free, direct and noncompetitive homogeneous FRET immunosensor was developed [84].…”

Section: Applicationsmentioning

confidence: 99%

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Zhang

et al. 2016

Toxins

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Mycotoxin contamination threatens health and life of humans and animals throughout the food supply chains. Many of the mycotoxins have been proven to be carcinogens, teratogens and mutagens. The reliable and sensitive sensing methods are requested to monitor mycotoxin contamination. Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing. These sensors are miniaturized, thereby lowering costs, and are applicable to high-throughput modes. In this work, the latest developments in sensing strategies for mycotoxin determination were critically discussed. Optical and electrochemical sensing modes were compared. The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors. This work might promote academic studies and industrial applications for mycotoxin sensing.

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Section: Applicationsmentioning

confidence: 99%

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Zhang

et al. 2016

Toxins

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“…From these two works, LODs of 2 pg mL −1 and 0.8 ng mL −1 were determined, while the detection ranges of 5 pg mL −1 -5 ng mL −1 and 1 ng mL −1 -100 ng −1 were achieved respectively. In addition, OTA aptasensors were also developed based on electrochemical differential-pulse voltammetry [22] and chemiluminescence resonance energy transfer (CRET) [43], resulting in LODs of 0.07 ng mL −1 and 0.22 ng mL −1 , respectively. In these works, OTA aptamer was conjugated with paramagnetic microparticle beads or DNAzyme.…”

Section: Quantitative Detection Of Ota In Lab Buffermentioning

confidence: 99%

An ultrasensitive aptasensor for detection of Ochratoxin A based on shielding effect-induced inhibition of fluorescence resonance energy transfer

Liu

Wang

et al. 2016

Sensors and Actuators B: Chemical

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“…Ochratoxin A (OTA) is a toxic secondary metabolite belonging to methylisocoumarin derivatives, which is mainly produced by several mold fungi in the genus of Aspergillus and Penicillium . It may cause severe risks in human beings such as embryotoxicity, teratogenicity, carcinogenicity, nephrotoxicity, immunotoxicity and hepatotoxicity . Moreover, it is metabolized in biological body very slowly .…”

Section: Introductionmentioning

confidence: 99%

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Wan

Zhang

Bai

et al. 2017

J of Separation Science

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A new, rapid, green, and cost-effective magnetic solid-phase extraction of ochratoxin A from red wine samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes as the absorbent. The polydopamine-coated magnetic multi-walled carbon nanotubes were fabricated with magnetic multi-walled carbon nanotubes and dopamine by an in situ oxidative self-polymerization approach. Transmission electron microscopy, dynamic light scattering, X-ray photoelectron spectroscopy and vibrating sample magnetometry were used to characterize the absorbents. Ochratoxin A was quantified with high-performance liquid chromatography coupled with fluorescence detection, with excitation and emission wavelengths of 338 and 455 nm, respectively. The conditions affecting the magnetic solid-phase extraction procedure, such as pH, extraction solution, extraction time, absorbent amount, desorption solution and desorption time were investigated to obtain the optimal extraction conditions. Under the optimized conditions, the extraction recovery was 91.8-104.5% for ochratoxin A. A linear calibration curve was obtained in the range of 0.1-2.0 ng/mL. The limit of detection was 0.07 ng/mL, and the limit of quantitation was 0.21 ng/mL. The recoveries of ochratoxin A for spiked red wine sample ranged from 95.65 to 100.65% with relative standard deviation less than 8%. The polydopamine-coated magnetic multi-walled carbon nanotubes showed a high affinity toward ochratoxin A, allowing selective extraction and quantification of ochratoxin A from complex sample matrixes.

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Detection of ochratoxin A (OTA) in coffee using chemiluminescence resonance energy transfer (CRET) aptasensor

Cited by 107 publications

References 34 publications

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

An ultrasensitive aptasensor for detection of Ochratoxin A based on shielding effect-induced inhibition of fluorescence resonance energy transfer

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

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