Detection of olive oil adulteration with linoleic acid-rich oils by reversed-phase high-performance liquid chromatography

Physico-chemical Characteristics of Papaya (Carica papaya L.) Seed Oil of the Hong Kong/Sekaki Variety

“…These two reports suggest that TAG profile of papaya seed oil is apparently similar to that of olive oil. According to previous report, olive oil is found to possess large amount of oleic acid rich TAG molecules 38 . Unlike polyunsaturated oils, oleic acid rich oils are highly regarded in food application mainly due to its thermal-oxidative stability.…”

Application of Chromatographic and Infra-Red Spectroscopic Techniques for Detection of Adulteration in Food Lipids: A Review

“…The use of RP-HPLC in the detection of oil adulteration gained much attention due to ease of sample preparation as well as the natural variations in fatty acid composition do not affect the characteristic TAG profile of several oils and fats [45]. This approach was particularly useful for detection of adulteration of virgin olive oil with various seed oils rich in linoleic acid [45] as well as vegetable oils mixed with lard [33]. In majority of the cases, vegetable oil adulterations with animal fats are generally found to cause some deviations mainly on the existing TAG molecular species of the TAG elusion profiles [36].…”

“…Adulteration of palm kernel oil with animal fats such as lard and beef tallow, however, was tend to cause additional TAG peaks visible even at adulteration level as low as 5% [36]. Kapoulas and Andrikopoulos [45] introduced a HPLC-method which enabled the detection of low levels of seed oils such as sunflower, soybean, cottonseed and corn oils as adulterants in olive oil. According to this…”

“…Vegetable oils of palm, Palm kernel, and canola Vegetable oils adulterated with lard TAG profiling of the lipids of different vegetable oils using HPLC couples with RI detector CANDISC analysis of TAG compositional data helped to discriminate oils contaminated with lard [36] Virgin olive oil Linoleic rich seed oils in virgin olive oil HPLC coupled with RI detector to TAG profiling of samples Tri-linoleoylglycerol (LLL) TAG peak was identified as a good biomarker to detect olive oil adulteration [45,46] Lipids of fried peanut, tempeh, chicken and beef Fried oils mixed with lard HPLC coupled with RI detector to TAG profiling The characteristic TAG profiling provided direct evidence for lard contamination in these products [30] Olive oil Hazel nut oil in olive oil Reversed-phase liquid chromatography coupled to gas chromatography (E)-5-methylhept-2-en-4-one was found to be a potential biomarker for the rapid recognition of olive oils adulterated with hazelnut oils [53] Olive oil and butter Milk fat mixed with palm oil / olive oil mixed with sunflower oil GLC coupled with FID detector to determine sterol compositional data Possible to detect the presence of sunflower oil in olive oil or of palm oil in milk fat, down to as low as 5% (w/w) [54] Olive oil Olive oil adulteration by other seed oils GLC analysis of sterols using polar capillary column…”

Silver Ion TLC of Minor Triacylglycerol Components for Unambiguous Detection of Adulteration of Olive Oil With Vegetable Oils

“…Now it is well known that data for triacylglycerol (TAG) composition of oils are more informative (than FA data) and provide a higher level of discrimination between the analyzed samples. [5][6][7][8][9][10][11][12][13][14][15] Methods for analysis and authentication have been reviewed recently. [16] All these approaches, however, do not account for the natural variations in the contents of FA, either in genuine olive oils or in the vegetable oils used for preparation of the model mixtures.…”