Detection of oncornavirus‐like particles in HeLa cells. I. Fine structure and comparative morphological classification

Gelderblom, Hans R.; Bauer, Heinz; Ogura, Hajime; Wigand, R.; Fischer, A.

doi:10.1002/ijc.2910130212

Cited by 56 publications

(32 citation statements)

References 19 publications

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“…Striations of the ring-like shell of retroviruses have been described previously by Gelderblom et al (1974) and Sarkar & Moore (1968) described a helical arrangement of the nucleoid in mouse mammary tumour viruses which had been disrupted by detergent treatment. The apparent absence of spikes on the immature particles seen by negative staining is difficuR to explain.…”

Section: Negative Staining Temmentioning

confidence: 57%

Electron Microscopy of Human Immunodeficiency Virus

Hockley¹,

Wood²,

Jacobs

et al. 1988

Journal of General Virology

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SUMMARYTwo cell lines of human T lymphocytes (H9 and CEM) chronically infected with isolates of human or simian immunodeficiency viruses were examined by electron microscopy. Scanning electron microscopy of H9 cells showed characteristic morphological changes in the cells after infection with human T cell lymphotropic virus type III (HTLV-IIIB); these included loss of cell microvilli and their replacement by rounded surface protrusions. Virus particles were present over the whole cell surface, but were more numerous between, rather than on, surface protrusions. In contrast, CEM cells infected with three other isolates of the virus showed little change in morphology compared with uninfected cells; they typically had a single large aggregate of virus particles at the posterior end of the cell. Transmission electron microscopy of sections of infected cells showed budding virus in only a small proportion of cells although many had mature virus particles on their surface. The immature particles released by budding had a ring-like morphology in contrast to the mature virus with its characteristic elongated nucleoid. Surface spikes were rarely seen on sectioned virus particles, but were more obvious on the simian immunodeficiency virus than on human isolates. Negative staining of purified virus preparations showed that the peripheral dense material of immature particles had a striated structure. Surface spikes were not seen by negative staining on either immature or mature virus particles. A third type of smaller particle with surface spikes was found in all negatively stained preparations.

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Section: Negative Staining Temmentioning

confidence: 57%

Electron Microscopy of Human Immunodeficiency Virus

Hockley¹,

Wood²,

Jacobs

et al. 1988

Journal of General Virology

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“…MPV and all other viruses used were purified from culture super natant fluids by equilibrium density gradient centri fugation and were concentrated as previously described [20], X-381 virus was purified from the supernatant fluid of X-381 cells established from a rhesus lactating mammary gland (3], LV was grown in bat lung cells (supplied by Dr. Todaro) [7], SMRV grown in canine Cf2Th cells cocultivated with squirrel monkey lung cells was supplied by Dr. R. Heberling and Dr.S.Kalter [6], Type-D retroviruses were purified from superna tant fluids of HeLa cells [10][11][12], from a cell line derived from a human amniotic cell, AO [13], and from two cell lines derived from human leukemic cells, T9 [14] and J96 [15]. Simian sarcoma virus I grown in NC-37 cells, baboon virus grown in BKCT (baboon kidney cocultivated with canine thymus) cells, and mouse mammary tumor virus grown in murine Mm5mt cells were obtained through the Biological Carcinogenesis Branch of the National Cancer Institute.…”

Section: Virus and Cellsmentioning

confidence: 99%

“…Viruses that are morphologically related to MPV have been identified in several human tissue culture cell lines, including HeLa cells [10][11][12] and cell lines established from amniotic cells (AO [13]) and leukemic cells (T9 [14]; J96 [15]). The AO, T9 and J96 cell lines were shown to have a type-A glucose-6-phosphate dehydrogenase isoenzyme and marker chro mosomes in common with HeLa cells [16].…”

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confidence: 99%

Immunological Characterization of Type-D Retrovirus Isolates from Human Cells

Colcher¹

1980

Intervirology

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Competition radioimmunoassays for the 27,000 molecular weight (p27) major internal protein and the low-molecular-weight 10,000–12,000 (p10–12) polypeptides of the primate type-D retroviruses, Mason-Pfizer virus (MPV) and langur virus (LV), were established. Type-D isolates from various primate and human tumor cells were examined in both these assays. There were no detectable immunological differences between MPV and two type-D retroviruses previously reported to be isolated from human cell lines (HeLa and AO). Type-D retroviruses isolated from human T9 and J96 cells (cell lines derived from human leukemic cells), however, were clearly distinguishable from both MPV and LV in their respective p10–12 radioimmunoassays. These assays thus demonstrate that type-D retroviruses isolated from some human cells may differ immunologically from morphologically similar viruses isolated from nonhuman primates.

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“…Moreover, viruses similar or identical to MPMV have also been reported in some isolates of HeLa cells (35)(36)(37) and the AO human cell line (38). The relationship, if any, between these isolates, the human milk particle, and MPMV remains to be determined.…”

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confidence: 92%