Detection of PAH:DNA adducts from auto-oxidation using 32P-postlabeling

Bryla, Piotr; Weyand, Eric H.

doi:10.1016/0304-3835(92)90210-m

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Indeed, TCDD has been reported to increase the BaP-DNA adducts in several experimental settings (Carvan et al, 1995;Harrigan et al, 2006;de Waard et al, 2008). Similar to BaP, DMBA itself can form high level of DNA adduct even in the absence of enzymatic or chemical activation (Bryla and Weyand, 1992). Under our experimental setting, DMBA enhanced the BaP-DNA adduct formation in a BRCA1-dependent manner.…”

Section: Resultsmentioning

confidence: 50%

The correlations between BRCA1 defect and environmental factors in the risk of breast cancer

Kang

Hong

et al. 2013

J. Toxicol. Sci.

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Section: Resultsmentioning

confidence: 50%

The correlations between BRCA1 defect and environmental factors in the risk of breast cancer

Kang

Hong

et al. 2013

J. Toxicol. Sci.

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Effects of UVA and visible light on the photogenotoxicity of benzo[a]pyrene and pyrene

Botta

Giorgio

Sabatier³

et al. 2008

Environmental Toxicology

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This study investigated the role of UVA/visible light (U, 320-800 nm) and visible light (V, 400-800 nm) in the phototoxicity and photogenotoxicity of two ubiquitous polycyclic aromatic hydrocarbons (PAH): benzo[a]pyrene (BaP) and Pyrene (Pyr). These mechanisms were evaluated by the WST-1 test and the comet assay on normal human keratinocytes (NHK) and by the micronucleus test on CHO cells. The production of reactive oxygen species (ROS) was assessed through the induction of 8-oxodeoxyguanine (8-oxodG) lesions by immunofluorescence staining in NHK. Results of the WST-1 test revealed the phototoxic properties of BaP and Pyr after irradiation with U and V lights. BaP presented the highest phototoxic properties. Results of the comet assay showed that U- and V-irradiated BaP and Pyr induced increasing rates of DNA single-strand breaks in NHK, in a dose dependent manner. The tested PAH could also induce increased levels of micronuclei in CHO cells after U and V irradiations. Increasing 8-oxodG levels were detected after U and V irradiations in BaP- and Pyr-treated keratinocytes and confirmed the involvement of ROS in the photogenotoxicity of PAH. Overall, this study highlighted the existence of an alternative pathway of PAH genotoxicity that is induced by UVA and/or visible light. Visible light is suggested to photoactivate PAH by a mechanism which is mainly based on oxidative reactions.

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Induction of in vivo DNA adducts by 4 industrial by-products in the rat-lung-cell system

Whong

Stewart

Cutler

et al. 1994

Mutation Research/Environmental Mutagenesis and Related Subject

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Detection of PAH:DNA adducts from auto-oxidation using 32P-postlabeling

Cited by 3 publications

References 22 publications

The correlations between BRCA1 defect and environmental factors in the risk of breast cancer

The correlations between BRCA1 defect and environmental factors in the risk of breast cancer

Effects of UVA and visible light on the photogenotoxicity of benzo[a]pyrene and pyrene

Induction of in vivo DNA adducts by 4 industrial by-products in the rat-lung-cell system

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