Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP)

Uematsu, Hiroshi; Inoue, Yasuhiro; Ohto, Yasuo

doi:10.1007/s10327-015-0580-4

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…Techniques include quantitative PCR (qPCR), variable number tandem repeat analysis (VNTR), loop-mediated isothermal amplication (LAMP), and multilocus sequence typing (MLST). 8,16,31,32 In general, these approaches depend on the ability to design PCR primers or associated reporter probes that are themselves specic only to the diagnostic section of interest, and may in principle be designed at any arbitrary level of taxonomic organisation (genus, species, subspecies, and so on). PCR-based methods have practical advantages, but in diagnostic use assumptions are made that the presence of target-specic DNA implies presence of the organism itself, and that the organism is viable.…”

Section: Diagnostic Methods For Srementioning

confidence: 99%

Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens

et al. 2016

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Section: Diagnostic Methods For Srementioning

confidence: 99%

Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens

et al. 2016

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“…Some plant diseases were attributed to only three species of Pantoea , namely P. agglomerans , P. ananatis and P. stewartii , which can therefore be considered as the major Pantoea species infecting plants. For their diagnosis, several PCR methods are available and have been used but some of them produced amplicons with others species as well [14,50,51], while others are not well reproducible or are inaccessible in typical sub-Saharan laboratory due to specific equipment requirements and/or high costs of some reagents [14,17,18]. Notably, most assays target only one Pantoea species or subspecies.…”

Section: Discussionmentioning

confidence: 99%

A diagnostic multiplex PCR scheme for identification of plant-associated bacteria of the genusPantoea

Kini

Agnimonhan

Dossa

et al. 2018

Preprint

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12 13 Background: The genus Pantoea forms a complex of more than 25 species, among which 14 several cause diseases of several crop plants, including rice. Notably, strains of Pantoea 15 ananatis and Pantoea stewartii have been found to cause bacterial leaf blight of rice in Togo 16 and Benin, while other authors have observed that Pantoea agglomerans can also cause 17 bacterial leaf blight of rice. The contribution of these and perhaps other species of Pantoea to 18 plant diseases and yield losses of crop plants is currently not well documented, partly due to 19 the lack of efficient diagnostic tools. 20 21 Result: Using 34 whole genome sequences of the three-major plant-pathogenic Pantoea 22species, a set of PCR primers that specifically detect each of the three species, 23 P. agglomerans, P. ananatis, and P. stewartii, was designed. A multiplex PCR protocol which 24 can distinguish these three species and also detects members of other Pantoea species was 25 2 further developed. Upon validation on a set of reference strains, 609 suspected Pantoea 26 strains that were isolated from rice leaves or seeds originating from 11 African countries were 27 screened. In total, 41 P. agglomerans strains from eight countries, 79 P. ananatis strains from 28 nine countries, 269 P. stewartii strains from nine countries and 220 unsolved Pantoea strains 29 from ten countries were identified. The PCR protocol allowed detecting Pantoea bacteria 30 grown in vitro, in planta and in rice seeds. The detection threshold was estimated at 5 ng/mL 31 of total genomic DNA and 1×10 5 CFU/mL of heated cells. 32 33 Conclusion: This new molecular diagnostic tool will help accurately diagnose major plant-34 pathogenic species of Pantoea. Due to its robustness, specificity, sensitivity, and cost 35 efficiency it will be very useful for plant protection services and for the epidemiological 36 surveillance of these important crop-threatening bacteria. 37 38 39 41 3 Background 42 43 The genus Pantoea was first described in 1989 and was recently taxonomically classified as a 44 member of the Erwiniaceae family [1]. More than 25 species of this genus have been 45 described and reported worldwide [2,3]. Etymologically, the genus name Pantoea is derived 46 from the Greek word 'Pantoios', which means "of all sorts or sources" and reflects the diverse 47 geographical and ecological sources from which the bacteria have been isolated. Several 48 species of the genus are qualified as versatile and ubiquitous bacteria because they have been 49 isolated from many different ecological niches and hosts [2,4]. Remarkably, some species 50 have the ability to colonize and interact with members of both the plant and the animal 51 Kingdom [5]. Among the plant-interacting species, Pantoea ananatis, Pantoea agglomerans 52 and Pantoea stewartii are well known for their phytopathogenic characteristics. They are 53 recognized as the causal agent of several diseases, such as leaf blight, spot disease, dieback, 54 grain discoloration, seed stalk rot, center rot, stem necros...

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“…Disease severity is directly related to the number of vectors that survive the winter (Roper, 2011). In Canada, the bacterium is reported to occur intermittently (Uematsu et al, 2015).…”

Section: Environmental Suitabilitymentioning

confidence: 99%

Pest survey card on Pantoea stewartii subsp. stewartii

Gaag¹,

Schenk²,

Delbianco³

et al. 2020

EFS3

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This pest survey card was prepared in the context of the EFSA mandate on plant pest surveillance (M‐2017‐0137), at the request of the European Commission. Its purpose is to assist the Member States in preparing their survey activities for Pantoeastewartiisubsp. stewartii using a statistically sound and risk‐based pest survey approach. Pantoeastewartiisubsp. stewartiiis a Union quarantine pest and the causal agent of Stewart's disease in maize crops. Maize is the only relevant host and P.stewartii subsp.stewartiiis expected to be able to become established in all maize growing areas of the EU.It is subject to specific measures to prevent entry of the pest via maize seeds, which are considered to be the main pathway for the introduction and spread of the bacterium. Natural spread in the EU through insect vectors is currently uncertain. Surveys in all Member States should be aimed at substantiating pest freedom and should take place once per year at a time when symptoms can be observed in maize crops. The detection should be performed through visual examination and, when symptomatic leaves are observed, they should be collected for laboratory testing. It is recommended that identification of P. stewartiisubsp.stewartiiis done by performing two independent identification methods.

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Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP)

Cited by 8 publications

References 15 publications

Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens

Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens

A diagnostic multiplex PCR scheme for identification of plant-associated bacteria of the genusPantoea

Pest survey card on Pantoea stewartii subsp. stewartii

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