Detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method

Urbán, Edit; Terhes, Gabriella; Radnai, Márta; Gorzó, István; Nagy, Elisabeth

doi:10.1016/j.anaerobe.2010.02.005

Cited by 28 publications

(29 citation statements)

References 11 publications

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“…18,19 For example, Eick and Pfister 19 compared periodontopathogenic microorganisms ( A. actinomycetemcomitans , P. gingivalis , P. intermedia , T. forsythia , T. denticola ) using the micro-IDent kit and the cultivation method in plaque samples taken from patients with aggressive periodontitis and periodontally healthy patients. According to the their results, the percentage of detection of A. actinomycetemcomitans , P. gingivalis , P. intermedia , T. forsythia and T. denticola in periodontally healthy patients were 12%, 12%, 8%, 8% and 2%, respectively, whereas these detection rates in patients with aggressive periodontitis were 20%, 33%, 52%, 68% and 50%, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

et al. 2013

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Oral lichen planus (OLP) is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 (18 chronic periodontitis (OLPP) and 9 gingivitis (OLPG)) patients diagnosed with OLP along with 26 (13 chronic periodontitis (HP) and 13 gingivitis (HG)) healthy non-OLP patients. The multiplex polymerase chain reaction (PCR) with subsequent reverse hybridization method (micro-IDent) was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients (OLPG and OLPP) were 18.5%, 85.1%, 81.4%, 88.8% and 74%, respectively. Meanwhile, in the non-OLP patients (HG and HP), these values were 7.6%, 50%, 46.1%, 73% and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens' percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathogenic microorganisms in the progress of periodontal diseases of OLP.

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Section: Discussionmentioning

confidence: 99%

“…However, the culture method is inefficient in the identification of anaerobic microorganisms. 18 Recently, various methods have been used in the assay of microorganisms. Molecular methods are used in the identification of microbial composition in subgingival plaque samples.…”

Section: Introductionmentioning

confidence: 99%

Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

et al. 2013

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“…Commercial diagnostic tests have been developed mainly for the detection of periodontopathogenic bacteria, including P. intermedia [202][203][204][205].…”

Section: Diagnosis Of Prevotella Spp Infectionsmentioning

confidence: 99%

New Insights into Prevotella Diversity and Medical Microbiology

Alauzet¹,

Marchandin

Lozniewski³

2010

Future Microbiol.

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In light of recent studies based on cultivation-independent methods, it appears that the diversity of Prevotella in human microbiota is greater than was previously assumed from cultivation-based studies, and that the implication of these bacteria in several human diseases was unrecognized. While some Prevotella taxa were found during opportunistic infections, changes in Prevotella abundance and diversity were discovered during dysbiosis-associated diseases. As member of the microbiota, Prevotella may also be considered as a reservoir for resistance genes. Greater knowledge on Prevotella diversity, as well as new insights into its pathogenic potential and implication in dysbiosis are expected from the use of human microbe identification microarrays, from whole-genome sequence analyse, and from the NIH Human Microbiome Project data. New approaches, including molecular-based methods, could contribute to improve the diagnosis of Prevotella infections.

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“…The informed consent was initially obtained from the subjects who were willing to participate in the study. A total of 120 subjects were selected and those with clinical signs of periodontal disease and diagnosed with chronic generalized periodontitis, with periodontal probing depth of ≥ 5-7mm and clinical attachment loss of ≥ 3mm were included in the study 16 . For standardization the same dentist carried out the sampling procedure.…”

Section: Methodsmentioning

confidence: 99%

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2017

IJPSR

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Detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method

Cited by 28 publications

References 11 publications

Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

New Insights into Prevotella Diversity and Medical Microbiology

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