Detection of plasma cell immunoglobulins in tissue sections optimally fixed for ultrastructural immunocytochemistry.

Gonatas, Nicholas K.; Gonatas, Jacqueline O.; Stieber, Anna; Ternynck, Thérèse; Avraméas, S

doi:10.1177/35.2.3098833

Cited by 12 publications

(5 citation statements)

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“…The restricted localization of the TGN to the cell body and the initial segment of one of the dendrites was confirmed by EM of internalized WGA-HRP ( Figure 2). HRP-conjugated lectins have been shown to be efficiently transported to one side of the Golgi apparatus of neuronal cells, which almost certainly represents the TGN (Gonatas et al, 1977). These results show that the Golgi complex and the TGN, as defined by these markers, are both localized exclusively to the cell body and the initial dendritic segment, as shown to be the case in neurons in situ (Peters et al, 1991) and in culture (de Camilli et al, 1986;Dotti and Simons, 1990;Lowenstein et al, 1994).…”

Section: Localization Of Golgi and Tgn Markersmentioning

confidence: 56%

The organization of the endoplasmic reticulum and the intermediate compartment in cultured rat hippocampal neurons.

Krijnse‐Locker

Parton

Fuller

et al. 1995

MBoC

138

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The boundaries of the organelles of the biosynthetic endomembrane system are still controversial. In this paper we take advantage of the unique architectural organization of neurons to investigate the localization of a spectrum of compartment-specific markers with the goal of defining the location of the rough endoplasmic reticulum (ER), smooth ER, intermediate compartment, and the Golgi complex. Markers of the rough ER (signal sequence receptor), Golgi complex (mannosidase II), and the trans Golgi network (TGN38) were essentially restricted to the cell body and the initial segment of one of the cell's dendrites. In contrast the cytochemical reaction product for glucose 6 phosphate, a classical ER marker, in addition to staining ER structures in the cell body also reacted with smooth ER elements that extended into both axons and dendrites. These peripheral smooth ER elements also reacted at the immunofluorescence level for ER marker 3-hydroxy-3-methylglutaryl-coenzyme A reductase, as well as for calnexin and protein disulfide isomerase. We also analyzed the location of rab1, rab2, p58, the KDEL receptor, and beta-subunit of coatomer. These intermediate compartment markers were found predominantly in the cell body but also extended to the proximal parts of the dendrites. Collectively, our data argue that the ER of hippocampal neurons consists of functionally and spatially distinct and separated domains, and they stress the power of the hippocampal neuron system for investigations of the organization of the ER by light microscopy.

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Section: Localization Of Golgi and Tgn Markersmentioning

confidence: 56%

The organization of the endoplasmic reticulum and the intermediate compartment in cultured rat hippocampal neurons.

Krijnse‐Locker

Parton

Fuller

et al. 1995

MBoC

138

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“…Underlying the cis-element, the next two or three saccules of the cis-compartment, perforated by wells as in other cell types (Hermo et al, 1980;Rambourg and Clermont, 1986;Rambourg et al, 1987a,b), were slightly distended with a pale-stained fluffy material. The morphological characteristics of these saccules were suggestive of their involvement in the accumulation of secretory material as also indicated by their reactivity for immunoglobulins (Tomonaga and Schoefl, 1975;Geuze and Slot, 1980;Hearn et al, 1985;Gonatas et al, 1987). Fig.…”

Section: The Cis-compartment Of the Golgi Apparatusmentioning

confidence: 74%

“…In the present investigation of the Golgi apparatus of plasma cells from the lamina propria of rat duodenum, dense granules were observed in the Golgi region either associated or not with the stacked elements of the organelle. Other authors have 0 1989 ALAN R. LISS, INC. also observed the presence of granules in the Golgi region of plasma cells (Tomonaga and Schoefl, 1975;Geuze and Slot, 1980;Hearn et al, 1985;Gonatas et al, 1987). The purpose of the present article is to examine the three-dimensional structure of the various elements that compose the Golgi apparatus and to analyze the mode of formation of typical secretion granules within the saccules of the Golgi stack.…”

Section: Introductionmentioning

confidence: 93%

Formation of secretion granules in the Golgi apparatus of plasma cells in the rat

Rambourg

Clermont²,

Hermo³

et al. 1989

Am. J. Anat.

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The three-dimensional structure of the components of the Golgi apparatus was analyzed in plasma cells of rat duodenum. The spheroidal juxtanuclear Golgi apparatus was formed by a continuous ribbonlike structure composed of the following stacked elements. On the cis-face of the Golgi stack, there was a tubular membranous network referred to as the cis-element and/or a slightly dilated saccule perforated with small pores. The two or three subjacent saccules, which showed few pores, were slightly dilated and contained a fluffy granulofilamentous material. They were also perforated in register by cavities or wells containing 80-nm vesicles. The next one or two underlying elements were fenestrated saccules showing flattened portions as well as distended portions containing a homogeneous material denser than that seen in the overlying saccules. The last two or three elements of the stack showed a partially separated or "peeling off" configuration. These last elements consisted of prosecretory granules attached to flattened, empty-looking saccules showing buds at their surface; detached, more-or-less fenestrated, flattened saccules; and shrivelled residual trans-tubular networks. In the trans-region of the stack, in addition to numerous small vesicles, short membranous tubules, detached prosecretory granules, and denser fully formed secretion granules were also seen. These images were interpreted to indicate that secretory material present in the trans-saccules flows toward the dilated portions which become prosecretory granules. The trans-most elements seemingly peel off the stack to yield prosecretory granules and fragmenting trans-tubular networks.

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“…Double-label electron microscopy can distinguish between these alternatives, but such studies have been rare. Endocytosed ligands may be localized, at least transiently, in structures with the cytochemical properties of TGN (68). Cell-surface markers have been shown in a few cases to enter into the cisternae of the Golgi stack and even into the newly formed secretory granules (6,69).…”

Section: Tgn and Membrane Recyclingmentioning

confidence: 98%

The trans Golgi Network: Sorting at the Exit Site of the Golgi Complex

Griffiths

Simons

1986

Science

1,165

597

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The Golgi complex is a series of membrane compartments through which proteins destined for the plasma membrane, secretory vesicles, and lysosomes move sequentially. A model is proposed whereby these three different classes of proteins are sorted into different vesicles in the last Golgi compartment, the trans Golgi network. This compartment corresponds to a tubular reticulum on the trans side of the Golgi stack, previously called Golgi endoplasmic reticulum lysosomes (GERL).

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Detection of plasma cell immunoglobulins in tissue sections optimally fixed for ultrastructural immunocytochemistry.

Abstract: endoplasmic reticulum, and the Golgi apparatus complex. In i Supported by a USPHS Grant NS 05572 and a Fulbright Grant to Materials and Methods Preparation of Immunoreagents and Fixation.

Cited by 12 publications

References 0 publications

The organization of the endoplasmic reticulum and the intermediate compartment in cultured rat hippocampal neurons.

The organization of the endoplasmic reticulum and the intermediate compartment in cultured rat hippocampal neurons.

Formation of secretion granules in the Golgi apparatus of plasma cells in the rat

The trans Golgi Network: Sorting at the Exit Site of the Golgi Complex

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