Detection of protein carbonyls in aging liver tissue: A fluorescence-based proteomic approach

Chaudhuri, Asish; Waal, Eric M. de; Pierce, Anson; Remmen, Holly Van; Ward, Walter F.; Richardson, Arlan

doi:10.1016/j.mad.2006.08.006

Cited by 139 publications

(125 citation statements)

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“…RGN is a Ca binding protein and is important for intracellular Ca homeostasis and in signaling pathways controlling glucose utilization and lipid production [18][19][20]. RGN is sensitive to oxidative modification and a more than two-fold increase in the carbonyl content has been reported in old mice [21]. RGN is also known as senescence marker protein 30 (SMP30) [22].…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

et al. 2007

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To identify biomarkers associated with the development of hepatocellular carcinoma (HCC) in CuZn superoxide dismutase (CuZnSOD, Sod1) deficient mice, 2-DE followed by MS analysis was carried out with liver samples obtained from 18-month-old Sod1−/− and +/+ mice. The intra-cellular Ca binding protein, regucalcin (RGN), showed a divergent alteration in Sod1−/− samples. Whereas elevated RGN levels were observed in −/− samples with no obvious neoplastic changes, marked reduction in RGN was observed in −/− samples with fully developed HCC. GST mu1 (GSTM1), on the other hand, showed a significant increase only in the neoplastic regions obtained from Sod1−/− livers. No change in GSTM1 was observed in the surrounding normal tissues. Marked reduction was observed in two intracellular lipid transporters, fatty acid binding protein 1 (FABP1) and major urinary protein 11 and 8 (MUP 11&8), in Sod1−/− samples. Analysis of additional samples at 18−22 months of age showed a three-fold increase in enolase activities in Sod1−/− livers. Consistent with previous findings, carbonic anhydrase 3 (CAIII) levels were significantly reduced in Sod1−/− samples, and immunohistochemical analysis revealed that the reduction was not homogenous throughout the lobular structure in the liver.

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Section: Discussionmentioning

confidence: 99%

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

et al. 2007

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“…Aer acquisition of the uorescence image, gels were stained with colloidal coomassie, 48 and gel images were acquired with a GS-800 Calibrated Densitometer (BioRad, Hercules, CA, USA). Protein carbonyls were labelled by adding (FTC) to tissue homogenates (100 mg) to a nal concentration of 1 mM (adapted from Chaudhuri et al 49 ). Samples were incubated for 2 h in the dark at 4 C before precipitation of proteins with a nal concentration of 10% w/v of TCA.…”

Section: Ptm Labelling and One-dimensional Electrophoresismentioning

confidence: 99%

Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna

Rainville

Carolan

Coelho

et al. 2014

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Access to the full text of the published version may require a subscription. Recent decades have seen a strong increase in the promise and uses of nanotechnology. This is correlated with their growing release in the environment and there is concern that nanomaterials may endanger ecosystems. Silver nanoparticles (AgNPs) have some of the most varied applications, making their release into the environment unavoidable. In order to assess their potential toxicity in aquatic environments, the acute toxicity of citrate-coated AgNPs to Daphnia magna was measured and compared to that of AgNO 3 . AgNPs were found to be ten times less toxic by mass than silver ions, and most of this toxicity was removed by ultracentrifuging. At the protein level, the two forms of silver had different impacts. RightsBoth increased protein thiol content, while only AgNP increased carbonyl levels. In 2DE of samples labelled for carbonyls, no feature was significantly affected by both compounds, indicating different modes of toxicity. Identified proteins showed functional overlap between the two compounds:vitellogenins (vtg) were present in most features identified, indicating their role as a general stress sensor.In addition to vtg, hemoglobin levels were increased by the AgNP exposure while 14-3-3 protein (a regulatory protein) carbonylation levels were reduced by AgNO 3 . Overall, this study confirms the previously observed lower acute toxicity of AgNPs, while demonstrating that the toxicity of both forms of silver follow somewhat different biologic pathways, potentially leading to different interactions with natural compounds or pollutants in the aquatic environment.

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“…The total protein carbonyl levels were measured by fluorescein-5-thiosemicarbazide (FTC) labeling of carbonyl groups in total muscle extract and separation by electrophoresis, as previously described (19). Briefly, the muscle from animals of different age was homogenized in deaerated buffer and centrifuged at 4°C for 1 h at 100,000 g. The resulting supernatant (cytosolic fraction) was treated with 1% streptomycin sulfate and incubated at 37°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Reduced mitochondrial ROS, enhanced antioxidant defense, and distinct age-related changes in oxidative damage in muscles of long-lived Peromyscus leucopus

Shi

Pulliam

Liu

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Van Remmen H. Reduced mitochondrial ROS, enhanced antioxidant defense, and distinct age-related changes in oxidative damage in muscles of long-lived Peromyscus leucopus. Am J Physiol Regul Integr Comp Physiol 304: R343-R355, 2013. First published January 16, 2013 doi:10.1152/ajpregu.00139.2012.-Comparing biological processes in closely related species with divergent life spans is a powerful approach to study mechanisms of aging. The oxidative stress hypothesis of aging predicts that longer-lived species would have lower reactive oxygen species (ROS) generation and/or an increased antioxidant capacity, resulting in reduced oxidative damage with age than in shorter-lived species. In this study, we measured ROS generation in the young adult animals of the long-lived white-footed mouse, Peromyscus leucopus (maximal life span potential, MLSP ϭ 8 yr) and the common laboratory mouse, Mus musculus (C57BL/6J strain; MLSP ϭ 3.5 yr). Consistent with the hypothesis, our results show that skeletal muscle mitochondria from adult P. leucopus produce less ROS (superoxide and hydrogen peroxide) compared with M. musculus. Additionally, P. leucopus has an increase in the activity of antioxidant enzymes superoxide dismutase 1, catalase, and glutathione peroxidase 1 at young age. P. leucopus compared with M. musculus display low levels of lipid peroxidation (isoprostanes) throughout life; however, P. leucopus although having elevated protein carbonyls at a young age, the accrual of protein oxidation with age is minimal in contrast to the linear increase in M. musculus. Altogether, the results from young animals are in agreement with the predictions of the oxidative stress hypothesis of aging with the exception of protein carbonyls. Nonetheless, the age-dependent increase in protein carbonyls is more pronounced in short-lived M. musculus, which supports enhanced protein homeostasis in longlived P. leucopus. Peromyscus leucopus; mitochondria; reactive oxygen species; skeletal muscle; oxidative damage; comparative biology of aging MAMMALIAN LIFE SPAN RANGES a hundred-fold, and comparative studies on long-lived animals can offer insights into key cellular mechanism(s) that may contribute to successful aging and longevity. The white-footed mouse, Peromyscus leucopus (Rodentia: Cricetidae), is one of the most common rodent species residing in North America. Both P. leucopus and the commonly used laboratory mouse, Mus musculus, belong to the superfamily Muridae and closely resemble each other in both body size and physical appearance. Yet P. leucopus can live more than 8 yr in captivity (35, 54), while the C57BL/6J strain of M. musculus lives up to 3.5 yr under standard laboratory conditions (Jax.org).In comparative aging studies, it is important to account for the effect of body size on species longevity, and it is calculated using the longevity quotient (LQ), which is the ratio of observed maximum life span as a function of that predicted allometrically using the equation ( Long-lived P. leucopus has caught the attention of biogerontolo...

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Detection of protein carbonyls in aging liver tissue: A fluorescence-based proteomic approach

Cited by 139 publications

References 70 publications

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna

Reduced mitochondrial ROS, enhanced antioxidant defense, and distinct age-related changes in oxidative damage in muscles of long-lived Peromyscus leucopus

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