Detection of Quantitative Trait Loci (QTLs) for Resistances to Small Brown Planthopper and Rice Stripe Virus in Rice Using Recombinant Inbred Lines

Abstract

Small brown planthopper (SBPH) and rice stripe virus (RSV) disease transmitted by SBPH cause serious damage to rice (Oryza sativa L.) in China. In the present study, we screened 312 rice accessions for resistance to SBPH. The indica variety, N22, is highly resistant to SBPH. One hundred and eighty two recombinant inbred lines (RILs) derived from a cross of N22 and the highly susceptible variety, USSR5, were used for quantitative trait locus (QTL) analysis of resistances to SBPH and RSV. In a modified seedbox screening test, three QTLs for SBPH resistance, qSBPH2, qSBPH3 and qSBPH7.1, were mapped on chromosomes 2, 3 and 7, a total explaining 35.1% of the phenotypic variance. qSBPH7.2 and qSBPH11.2, conferring antibiosis against SBPH, were detected on chromosomes 7 and 11 and accounted for 20.7% of the total phenotypic variance. In addition, qSBPH5 and qSBPH7.3, expressing antixenosis to SBPH, were detected on chromosomes 5 and 7, explaining 23.9% of the phenotypic variance. qSBPH7.1, qSBPH7.2 and qSBPH7.3, located in the same region between RM234 and RM429 on chromosome 7, using three different phenotyping methods indicate that the locus or region plays a major role in conferring resistance to SBPH in N22. Moreover, three QTLs, qSTV4, qSTV11.1 and qSTV11.2, for RSV resistance were detected on chromosomes 4 and 11. qSTV11.1 and qSTV11.2 are located in the same region between RM287 and RM209 on chromosome 11. Molecular markers spanning these QTLs should be useful in the development of varieties with resistance to SBPH and RSV.

The rice serine/threonine protein kinase OsPBL1 (ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1) is potentially involved in resistance to rice stripe disease

“…On the other hand, several resistance QTLs to small brown planthopper (SBPH) have also been reported to be located on chromosomes 1, 2, 3, 4, 7, 11, and 12. Wang et al (2013) compared all known QTLs for their effects against SBPH and concluded that the QTLs located on chromosomes 7, 11, and 12 exert major roles in resistance. Nonetheless, the exact molecular functions of most RSV resistance and/or SBPH resistance QTLs have not yet been reported.…”

Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice (Oryza sativa L.)

“…Small brown planthopper transmits virus disease (such as RBSDVD and RSV) and causes a direct damage to the plant. In previous studies, more QTLs against SBPH have been mapped using several populations (Duan et al., , ; Duan, Wan et al., ; Duan, Zhang et al., ; Sun et al., ; Tuyen et al., 2012; Wang et al., ; Zhang et al., ). Compared with these QTLs, qSBPH7 WR24 on chromosome 7 was located in the same interval as qSBPH7 .…”

“…Compared with these QTLs, qSBPH7 WR24 on chromosome 7 was located in the same interval as qSBPH7 . It is noteworthy that qSBPH7 has been repeatedly detected by three different phenotypic methods from a SBPH resistance cultivar N22 (Wang et al., ), suggesting qSBPH7 WR24 will be a reliable QTL for SBPH resistance breeding. Except qSBPH7 WR24 , the other two SBPH resistance QTLs, viz.…”

“…The number of insects on each seedling was counted 24 hr after infestation at 9:00 a.m. every day, and then, the insects were dispersed evenly among the seedlings after counting (Nemoto, Ishikawa, & Shimura, ). The average number of insects on each variety was calculated and treated as the antixenosis value with 5 days scoring (Duan, Wan et al., ; Duan, Zhang et al., ; Wang et al., ). The experiments were repeated three times.…”

“…In addition to find the RBSDV resistance genes, identification and utilization of SBPH resistance gene should be a complement strategy for controlling RBSDV. So far, about thirty QTLs for SPBH resistance derived from different rice varieties have been mapped onto different rice chromosomes (Duan, Cheng, Lei, Zhai, & Wan, ; Duan, Wan et al., ; Duan, Zhang et al., ; Wang et al., ) . However, none of the SBPH resistance gene has been fine‐mapped and cloned until now.…”