Detection of Quinoline in G. boninense-Infected Plants Using Functionalized Multi-Walled Carbon Nanotubes: A Field Study

Abstract

Carbon nanotubes (CNTs) reinforced with gold nanoparticles (AuNPs) and chitosan nanoparticles (CTSNPs) were anchored on a screen-printed electrode to fabricate a multi-walled structure for the detection of quinoline. The surface morphology of the nanocomposites and the modified electrode was examined by an ultra-high resolution field emission scanning electron microscope (FESEM), and Fourier-transform infrared (FT-IR) spectroscopy was used to confirm the presence of specific functional groups on the multi-walled carbon nanotubes MWCNTs. Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were used to monitor the layer-by-layer assembly of ultra-thin films of nanocomposites on the surface of the electrode and other electrochemical characterizations. Under optimized conditions, the novel sensor displayed outstanding electrochemical reactivity towards the electro-oxidation of quinoline. The linear range was fixed between 0.0004 and 1.0 μM, with a limit of detection (LOD) of 3.75 nM. The fabricated electrode exhibited high stability with excellent sensitivity and selectivity, specifically attributable to the salient characteristics of AuNPs, CTSNPs, and MWCNTs and the synergistic inter-relationship between them. The newly developed electrode was tested in the field. The Ipa increased with an increase in the amount of quinoline solution added, and the peak potential deviated minimally, depicting the real capability of the newly fabricated electrode.

An NMR Metabolomics Approach and Detection ofGanoderma boninense-Infected Oil Palm Leaves Using MWCNT-Based Electrochemical Sensor

“…Unevenness of the surface was observed in their finding after activation due to the attachment of carboxylic functional groups to the surface. The surface of aMWCNT was craggier with the incorporation of AuNPs which confirm successful loading of AuNPs on the aMWCNT [22].…”

“…The pristine-MWCNT shows a uniform and equivalent tubular arrangement with some voids. The tubes were not shortened on activation even the spaces are closed up to an extent [22,43]. Unevenness of the surface was observed in their finding after activation due to the attachment of carboxylic functional groups to the surface.…”

“…Electrode. The modified electrode was prepared according to the method described previously [18][19][20][21][22]. Layer-by-layer (LBL) assembly was employed to modify the electrode The bare screen-printed carbon electrode (SPCE) was rinsed several times with deionized water and immersed into gold nanoparticle-carbon nanotube (AuNP-CNT) dispersion for 5 min.…”

“…Days Postinfection. Our coauthor has characterized previously the morphological structures of MWCNT and AuNP-aMWCNT composites using an ultrahigh resolution field emission scanning electron microscope (FESEM) [22]. The pristine-MWCNT shows a uniform and equivalent tubular arrangement with some voids.…”

Sensitive and Selective Detection of DNA Fragments Associated with Ganoderma Boninense by DNA-Nanoparticle Conjugate Hybridisation

“…14 Further, their application in the routine analysis is limited by their complex protocols, labile reagents with high cost, relatively low sensitivity and the requirement of highly skilled personnel. Electrochemical methods for the detection of quinoline (a secondary metabolite produced by the palms under G. boninense attack), 16 and DNA fragments associated with this organism 17,18 have also been reported. The latter in particular has been shown to give high sensitivity (~ 1 fM of target DNA) and selectivity towards G. boninense.…”