Detection of ‘rare event’ fetal erythroblasts in maternal blood using automated microscopy

Tanke, Hans J.; Oosterwijk, Jan C.; Mesker, Wilma E.; v-Velzen, M.C.M. Ouwerkerk; Knepflé, Cecile F. H. M.; Wiesmeyer, C.C.; Ommen, G.J.B. van; Kanhai, Humphrey H.H.; Vrolijk, J.

doi:10.1016/s0378-3782(96)01829-4

Cited by 22 publications

(23 citation statements)

References 4 publications

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“…Furthermore, the new automated scanning microscope and image analysis system ARIOL SL-50 allows a reliable quantification of the number of cells detected for each patient. A precursor model of this equipment has already been used to analyze fetal erythrocytes in maternal blood in 1996 (28).…”

Section: Discussionmentioning

confidence: 99%

Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Hoffmann

Beelen

Gellhaus

et al. 2008

Hypertension in Pregnancy

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Section: Discussionmentioning

confidence: 99%

Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Hoffmann

Beelen

Gellhaus

et al. 2008

Hypertension in Pregnancy

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“…In this way, cell density was controllable, which provides many cells per field with minimal cell overlap. The cells are flattened, which facilitates the use of autofocus, and the cell-containing area on the slide is of constant size and location, all of which facilitate eventual automated microscopic screening (32).…”

Section: Discussionmentioning

confidence: 99%

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Oosterwijk¹,

Mesker²,

Velzen³

et al. 1998

Cytometry

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“…These include both flow cytometry-based techniques and solid-phase image scanning cytometry techniques (3,4,(6)(7)(8). The latter usually uses a planar multielement photodetector, such as a charge-coupled device (CCD) camera, to make images from adjacent elements across the specimen, followed by scene segmentation and use of pattern recognition algorithms to locate the particular targets (9)(10)(11)(12)(13)(14)(15). Although it has the potential to detect rare events in an automatic way, presently, there exist several restrictions that limit its practical application.…”

mentioning

confidence: 99%

Automated detection of rare‐event pathogens through time‐gated luminescence scanning microscopy

Lu¹,

Jin²,

Leif³

et al. 2011

Cytometry Pt A

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Many microorganisms have a very low threshold (\10 cells) to trigger infectious diseases, and, in these cases, it is important to determine the absolute cell count in a lowcost and speedy fashion. Fluorescent microscopy is a routine method; however, one fundamental problem has been associated with the existence in the sample of large numbers of nontarget particles, which are naturally autofluorescent, thereby obscuring the visibility of target organisms. This severely affects both direct visual inspection and the automated microscopy based on computer pattern recognition. We report a novel strategy of time-gated luminescent scanning for accurate counting of rare-event cells, which exploits the large difference in luminescence lifetimes between the lanthanide biolabels, [100 ls, and the autofluorescence backgrounds, \0.1 ls, to render background autofluorescence invisible to the detector. Rather than having to resort to sophisticated imaging analysis, the background-free feature allows a single-element photomultiplier to locate rare-event cells, so that requirements for data storage and analysis are minimized to the level of image confirmation only at the final step. We have evaluated this concept in a prototype instrument using a 2D scanning stage and applied it to rare-event Giardia detection labeled by a europium complex. For a slide area of 225 mm 2 , the time-gated scanning method easily reduced the original 40,000 adjacent elements (0.075 mm 3 0.075 mm) down to a few ''elements of interest'' containing the Giardia cysts. We achieved an averaged signal-to-background ratio of 41.2 (minimum ratio of 12.1). Such high contrasts ensured the accurate mapping of all the potential Giardia cysts free of false positives or negatives. This was confirmed by the automatic retrieving and time-gated luminescence bioimaging of these Giardia cysts. Such automated microscopy based on time-gated scanning can provide novel solutions for quantitative diagnostics in advanced biological, environmental, and medical sciences. ' 2011 International Society for Advancement of Cytometry

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Detection of ‘rare event’ fetal erythroblasts in maternal blood using automated microscopy

Cited by 22 publications

References 4 publications

Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Development of a preparation and staining method for fetal erythroblasts in maternal blood: Simultaneous immunocytochemical staining and FISH analysis

Automated detection of rare‐event pathogens through time‐gated luminescence scanning microscopy

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