Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification

Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility

“…The significant economic importance of T. ovatus is recognized due to its fast growth rate, pleasing flavour and growing market demand. Frequent outbreaks of viral and bacterial diseases, however, have occurred frequently in cultured T. ovatus since 2001 and caused great economic losses (Xu et al, ; Zhen et al, ). Given that the newly established cell lines will contribute greatly to investigate the molecular mechanism of virus or bacterial infection in vitro (Huang et al, 2011 a , b ), the development of cell lines originated from T. ovatus were urgently needed.…”

“…Pathogenic bacteria including Pseudomonas maltophilia , Nocardia seriolae , Vibrio damsel and Vibrio vulnificus have been reported to infect this fish (Zhou et al, ; Zhao et al, ; Huang et al, ; Wang et al, ; Xia et al, ). Red‐spotted grouper nervous necrosis virus (RGNNV) has been detected in the brain of T. ovatus , which shows typical symptoms of NNV infection (Xu et al, ). Fish‐derived cell lines have attracted much attention and are considered as powerful tools for study in epidemiology, toxicology, molecular carcinogenesis, pathology, endocrinology and immunology (Powers, ; Bols & Lee, ; Huang et al, 2009 a , b , 2011 b ; Ou‐Yang et al, ; Gong et al, ).…”

Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)

“…Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles Introduction Groupers, which are widely distributed throughout the tropical and subtropical waters of the world, are economically important marine fish and ideal study models for development and reproduction (Zhou et al 2007;Zhou and Gui 2010). Due to the development of intensive aquaculture industry in recent years, infectious diseases caused by viruses, bacteria, and parasites have become major constraints in grouper farming, of which viruses are the most devastating and most widely reported infectious agents for cultured groupers (Chi et al 1999;Lai et al 2003;Xu et al 2010). Iridovirus infections have brought high mortality rate in groupers, which resulted in significant economic losses (Qin et al 2006).…”

Nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products

“…Analysis of PCR products (amplicons) is, usually, carried out by agarose gel electrophoresis, followed by ethidium bromide staining. Several methodologies for rapid and accurate nodavirus detection have been developed recently, including: real-time quantitative PCR [18]; loop-mediated-isothermal-amplification (LAMP) [19] and real-time LAMP [20] assays; an immunomagnetic reduction (IMR) assay which employs magnetic nanoparticles coated with dextran and antibodies [21]; a microfluidic LAMP system, needing slab-electrophoresis for products detection [22]; a RT-PCR microfluidic chip with laser-induced fluorescence technology on the capillary electrophoresis module of the chip [23]; and an integrated M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT …”

“…Real-time PCR [18,47,48,[50][51][52][53] is less time consuming but requires more expensive instrumentation and/or reaction reagents. LAMP amplification methods with gel electrophoresis detection [19,45,46] or real time [20] format have also been applied on nodavirus detection. Real time nucleic acid sequence based amplification (NASBA) [54], RT-PCR combined with dot-plot detection [7], a LAMP-based microfluidic devise [22], a RT-PCR microfluidic devise with capillary electrophoresis detection (CE) [23] and a microfluidic device based on molecular beacons and magnetic separation [24] have, also, been utilized for nodavirus analysis.…”