Detection of Rice Tungro Bacilliform Virus by Polymerase Chain Reaction for Assessing Mild Infection of Plants and Viruliferous Vector Leafhoppers

Takahashi, Yuji K.; Tiongco, E. R.; Cabauatan, P. Q.; Koganezawa, H.; Hibino, H.; Omura, Toshihiro

doi:10.1094/phyto-83-655

Cited by 31 publications

(14 citation statements)

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“…The amphfication of viral DNA from CSSV-IAinfected cacao plants by IC-PCR was specific and highly sensitive. The dilution endpoint of infected plant sap was approx, 100-1000-fold lower than that routinely obtained by ELISA, demonstrating a similar threshold of virus detection as for the badnavirus RTBV (Takahashi et al, 1993). The application of IC-PCR for CSSV-l A detection in leaf samples collected from naturallyinfected trees in cacao farms in the Eastern Region and Ashanti Region of Ghana confirmed the superior sensitivity of IC-PCR over ELISA.…”

Section: Discussionmentioning

confidence: 53%

Immunocapture Polymerase Chain Reaction for the Detection and Characterization of Cacao Swollen Shoot Virus 1 A Isolates

Hoffmann¹,

Sackey

Maiß³

et al. 1997

Journal of Phytopathology

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Oligonucleotides derived from the flanking regions of the putative coat protein gene of the cacao swollen shoot badnavirus isolate 1 A (CSSV-l A) were able to prime the synthesis of specific products directly from extracts from CSSV-l A-infected leaves by immunocapture polymerase chain reaction (IC-PCR), following trapping of virions with polyclonal antibodies to CSSV-l A. CSSV isolates serologically distinct from CSSV-l A were not detected by IC-PCR when the CSSV-l A-derived primers were used following trapping with homologous antisera. IC-PCR was at least 100-fold more sensitive than double antibody sandwich (DAS)-ELISA in comparative tests on samples from greenhouse-grown cacao plants. The superior sensitivity of IC-PCR over DAS-ELISA was confirmed in attempts to detect and identify CSSV-l A isolates in field samples and permitted detection of CSSV-l A isolates even in symptomless leaves from plants showing stem swelling only. The IC-PCR products obtained from four randomly selected field samples were sequenced and shown to contain a region of the CSSV-1 A genome where ORF X overlaps ORF 3. Analysis of the partial amino acid sequences deduced from ORF 3 and ORF X of the four field isolates revealed a considerable variation in these CSSV-l A gene products.

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Section: Discussionmentioning

confidence: 53%

Immunocapture Polymerase Chain Reaction for the Detection and Characterization of Cacao Swollen Shoot Virus 1 A Isolates

Hoffmann¹,

Sackey

Maiß³

et al. 1997

Journal of Phytopathology

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“…The PCR was shown to be 10 3 –10 4 times more sensitive for detecting RTBV DNA than enzyme‐linked immunosorbent assay (ELISA) (Takahashi et al. ). The detection of RTBV at different days after inoculation clearly suggests that virus presence and symptoms score are not very much correlated.…”

Section: Discussionmentioning

confidence: 99%

The Differential Reaction of Rice Hybrids to Tungro Virus by Phenotyping and PCR Analysis

Singh

Ponnuswamy

Donempudi

et al. 2015

Journal of Phytopathology

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Twenty popular rice hybrids were used to screen for rice tungro virus (RTV) disease reaction. Virulent green leafhoppers (GLH) were used as vector to introduce RTV to the rice hybrids. Virus symptoms scores were recorded at 14, 21, 34, 41 and 59 days postinoculation (DPI), which suggested that virus symptoms are greatly influenced by growth stage of plants. To confirm the presence of virus, polymerase chain reaction (PCR)-based detection of Rice tungro bacilliform virus (RTBV) was carried out at 7, 14, 21 and 59 DPI using virus genome-specific primers. Virus presence was observed in all the rice hybrids and check varieties, particularly at later stages of infection. This study shows that phenotyping for tungro virus resistance in rice hybrids at 21 DPI gives most reliable results based on both virus symptoms and presence of virus. Further, to assess the relative difference in population of RTBV, quantitative PCR was performed in all the genotypes at 21 DPI. Yield data were also recorded from control and virus-infected plants to estimate yield loss percentage due to tungro disease. This study is important to understand the response of rice hybrids to tungro virus disease. Results obtained in this study emphasize that molecular detection of virus is very important to screen the rice plants accurately for tungro disease reaction.

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“…ELISA with PYMV always gave low absorbance values, and did not separate PYMV‐infected and healthy plants successfully. Other members of the badnavirus group also tend to have low absorbance values, for example, Sugarcane bacilliform virus (SCBV), Rice tungro bacilliform virus (RTBV) and Cocoa swollen shoot virus (S. Ganoo, Sugarcane Research Institute, Mauritius, personal communication, 1995; Hughes & Ollennu, 1993; Takahashi et al ., 1993). Despite the problems, ELISA is routinely used to evaluate rice and sugarcane cultivars for badnavirus infections.…”

Section: Discussionmentioning

confidence: 99%

Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri Lanka

2002

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Sri Lankan black pepper with symptoms of yellow mottle disease contained a mixture of viruses: Piper yellow mottle virus (PYMV) particles (30 × 130 nm), Cucumber mosaic virus (CMV, 30 nm diameter isometric particles), and unidentified, isometric virus-like particles (30 nm diameter). An effective purification procedure is described for PYMV. Immunosorbent and conventional electron microscopy successfully detected badnavirus particles only when at least partially purified extracts were used. PYMV was confirmed as the cause of the disease, with the other two viruses apparently playing no part in producing symptoms. PYMV was transmitted by grafting, by the insect vectors citrus mealy bug ( Planococcus citri ) and black pepper lace bug ( Diconocoris distanti ), but not by mechanical inoculation or through seeds. The CMV isolate was transmitted to indicator plants by mechanical inoculation and by the vector Aphis gossypii , but not by Myzus persicae ; but neither mechanical nor insect transmission of CMV to black pepper was successful. A sensitive polymerase chain reaction assay was developed to detect PYMV in black pepper.

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Detection of Rice Tungro Bacilliform Virus by Polymerase Chain Reaction for Assessing Mild Infection of Plants and Viruliferous Vector Leafhoppers

Cited by 31 publications

References 0 publications

Immunocapture Polymerase Chain Reaction for the Detection and Characterization of Cacao Swollen Shoot Virus 1 A Isolates

Immunocapture Polymerase Chain Reaction for the Detection and Characterization of Cacao Swollen Shoot Virus 1 A Isolates

The Differential Reaction of Rice Hybrids to Tungro Virus by Phenotyping and PCR Analysis

Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri Lanka

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