Detection of rodent liver carcinogen genotoxicity by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow)

Sasaki, Yu; Izumiyama, Fusako; Nishidate, Emi; Matsusaka, Naonori; Tsuda, Shuji

doi:10.1016/s1383-5718(97)00072-7

Cited by 90 publications

(30 citation statements)

References 19 publications

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“…2,4-DAT was positive in all assays, suggesting that this compound induced DNA damage and DNA repair in HepG2 cells. The positive results obtained with the comet assay agree with the in vivo study by Sasaki et al (1997). They show that 2,4-DAT at 240 mg/kg caused DNA damage in the liver and kidney 3 and 24 h after treatment; the comet tail was longer after 24 h than 3 h, suggesting that the DNA damage in these organs is time-dependent.…”

Section: Discussionsupporting

confidence: 86%

2,4-Diaminotoluene (2,4-DAT)-induced DNA damage, DNA repair and micronucleus formation in the human hepatoma cell line HepG2

et al. 2005

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Section: Discussionsupporting

confidence: 86%

2,4-Diaminotoluene (2,4-DAT)-induced DNA damage, DNA repair and micronucleus formation in the human hepatoma cell line HepG2

et al. 2005

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“…On Day 7 of gestation, 4 successfully copulated females were assigned to each treatment and non-treated control group (4, 8, 12 and 24 hr). In our previous mouse Comet assays, we observed no significant differences in mean migration between vehicle control groups and corresponding untreated groups at any time for any organs [15][16][17][18]. The results enabled us to use untreated control animals as concurrent control animals.…”

Section: Animalsmentioning

confidence: 66%

Detection of in vivo DNA Damage Induced by Ethanol in Multiple Organs of Pregnant Mice Using the Alkaline Single Cell Gel Electrophoresis (Comet) Assay

Kido

Sato

Tsuda

2006

The Journal of Veterinary Medical Science

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ABSTRACT. Ethanol is principal ingredient of alcohol beverage, but considered as human carcinogen, and has neurotoxicity. Alcohol consumption during pregnancy often causes fetal alcohol syndrome. The DNA damage is one of the important factors in carcinogenicity or teratogenicity. To detect the DNA damage induced by ethanol, we used an in vivo alkaline single cell gel electrophoresis (Comet) assay in pregnant mice organs and embryos. Pregnant ICR mice on Day 7 of gestation were treated with 2, 4 or 8 g/kg ethanol, and maternal organs/tissues and embryos were subjected to the Comet assay at 4, 8, 12 and 24 hr after ethanol treatment. Four and 8 g/kg ethanol induced DNA damage in brain, lung and embryos at 4 or 8 hr after the treatment. Two g/kg ethanol did not cause any DNA damage, and 8 g/kg ethanol only increased the duration of DNA damage without distinct increase in the degree of the damage. No significant DNA damage was observed in the liver. To detect the effect of acetaldehyde, disulfiram, acetaldehyde dehydrogenase inhibitor, was administered before 4 g/kg ethanol treatment. No significant increase of DNA damage was observed in the disulfiram pre-treated group. These data indicate that ethanol induces DNA damage, which might be related to ethanol toxicity. Since pre-treatment of disulfiram did not increase DNA damage, DNA damage observed in this study might not be the effect of acetaldehyde. KEY WORDS: Comet assay, DNA damage, ethanol, pregnant mouse.

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“…Фенобарбитал продемонстрировал также анеугенное действие в тес-те на S. Cerevisiae (Albertini et al, 1985). В двух неза-висимых исследованиях, методом ДНК-комет была показана ДНК-повреждающая активность фенобар-битала в клетках печени in vivo (Sasaki et al, 1997;Rothfuss et al, 2010). Вышеуказанное в очередной раз обозначает важность проблемы генотоксикологических исследований -оценки органо-и тканеспецифичности действия.…”

Section: результаты и обсуждениеunclassified

Comutagenic effects of valocordin

Дурнев

Даугель-Дауге

Жанатаев

et al. 2012

Ecological genetics

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The chromosome aberration test in bone marrow cells of mice was used to study the influence of valocordin on spontaneous and induced clastogenesis. Valocordin showed no inherent clastogenic activity. In experiments with pretreatment and with single or repeated combined administration , valocordin in doses of 0.03, 0.3 and 3 ml/kg significantly (47–133 %) enhanced the clastogenic activity of cyclophosphamide. There was no effect of valocordin on the cytogenetic activity of dioxidine, a mutagen with a pro-oxidative mode of action. Possible mechanisms of comutagenic activity of valocordin are discussed.

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Detection of rodent liver carcinogen genotoxicity by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow)

Cited by 90 publications

References 19 publications

2,4-Diaminotoluene (2,4-DAT)-induced DNA damage, DNA repair and micronucleus formation in the human hepatoma cell line HepG2

2,4-Diaminotoluene (2,4-DAT)-induced DNA damage, DNA repair and micronucleus formation in the human hepatoma cell line HepG2

Detection of in vivo DNA Damage Induced by Ethanol in Multiple Organs of Pregnant Mice Using the Alkaline Single Cell Gel Electrophoresis (Comet) Assay

Comutagenic effects of valocordin

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